Homozygous vesicle-associated membrane protein 2 (formerly synaptobrevin 2) KO mice show decreased synaptic vesicle fusion and die shortly after birth.
Dr. Thomas C. Sudhof, Stanford University School of Medicine
Mice homozygous for this targeted mutation die shortly after birth. Heterozygotes suffer no apparent morbidity or premature mortality. Newborn homozygotes exhibit a rounded appearance with a shoulder hump that may be caused by excess brown fat in the upper back. No developmental changes were noted. Analysis of brain sections did not detect any abnormalities or neurodegeneration. No changes are observed in the expression of a series of synaptic proteins. Spontaneous synaptic vesicle fusion and fusion induced by hypertonic sucrose are decreased approximately 10-fold, and fast Ca2+-triggered fusion was decreased more than 100-fold. No protein product from the targeted gene is detected in forebrain tissue. This mutant mouse strain represents a model that may be useful in studies of synaptic fusion mechanisms.
A targeting vector containing a floxed neomycin resistance gene was used to disrupt sequence 104 bp upstream of the initiator ATG. Point mutations (V38R and V42R) were introduced into the coding region. The construct was electroporated into 129P2/OlaHsd –derived E14.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. Chimeric mice were crossed with C57BL/6 mice. The strain has been backcrossed to C57BL/6 for several generations by the donating laboratory.
|Allele Name||targeted mutation 1, Thomas C Sudhof|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||syb 2 -; synaptobrevin/VAMP2-|
|Gene Symbol and Name||Vamp2, vesicle-associated membrane protein 2|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||A loxP-flanked neomycin resistance cassette was inserted 104 bp upstream of the initiation codon. Western blot analysis on brain extracts derived from homozygous mice confirmed that no detectable protein is produced from this allele.|
|Mutations Made By|| |
Dr. Thomas Sudhof, Stanford University School of Medicine
When maintained as a live colony, heterozygotes are bred with wildtype siblings. Homozygotes are not viable.
When using the Synaptobrevin 2 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #006380 in your Materials and Methods section.