These Scamp1 knock-out mice may be useful in studies relating to secretory vesicle exocytosis or regulation of endocytosis after formation of fusion pores.
Dr. Thomas C. Sudhof, Stanford University School of Medicine
Mice homozygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. Homozygotes exhibit no changes in the overall architecture or the protein composition of the brain or alterations in peripheral organs. No protein product from the targeted gene is detected in hippocampal tissue or peritoneal mast cells. No changes in the structure or composition of the brain were detected. Capacitance recordings of mast cells indicate that GTP(gamma)S-triggered exocytosis in targeted mice occurs as readily as in wildtype mice. This mutant mouse strain represents a model that may be useful in studies of secretory vesicle exocytosis.
A targeting vector containing a neomycin resistance gene was used to replace exons 3 and 4 encoding residues 46-114 of the gene. The construct was electroporated into 129S6/SvEvTac-derived SM1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric animals were crossed with C57BL/6. The strain has been minimally backcrossed to C57BL/6 by the donating laboratory.
|Allele Name||targeted mutation 1, Thomas C Sudhof|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Scamp1, secretory carrier membrane protein 1|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||Exons 3 and 4 were replaced with a neomycin selection cassette inserted by homologous recombination. The deleted region encoded amino acids 46 through 144. No protein was detected in homozygous mutant mice by Western blot analysis.|
|Mutations Made By|| |
Dr. Thomas Sudhof, Stanford University School of Medicine
Homozygotes are viable and fertile. When maintaining a live colony, homozygous mice may be bred together.
When using the SCAMP1 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #006379 in your Materials and Methods section.