B6;129S6-Scamp1^tm1Sud/J

Stock number: 006379
Product name: SCAMP1 KO
Research areas: Neurobiology Research

Description

These Scamp1 knock-out mice may be useful in studies relating to secretory vesicle exocytosis or regulation of endocytosis after formation of fusion pores.

Detailed description

Mice homozygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. Homozygotes exhibit no changes in the overall architecture or the protein composition of the brain or alterations in peripheral organs. No protein product from the targeted gene is detected in hippocampal tissue or peritoneal mast cells. No changes in the structure or composition of the brain were detected. Capacitance recordings of mast cells indicate that GTP(gamma)S-triggered exocytosis in targeted mice occurs as readily as in wildtype mice. This mutant mouse strain represents a model that may be useful in studies of secretory vesicle exocytosis.

Development

A targeting vector containing a neomycin resistance gene was used to replace exons 3 and 4 encoding residues 46-114 of the gene. The construct was electroporated into 129S6/SvEvTac-derived SM1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric animals were crossed with C57BL/6. The strain has been minimally backcrossed to C57BL/6 by the donating laboratory.

Allele

Symbol: Scamp1^tm1Sud
Name: targeted mutation 1, Thomas C Sudhof
MGI accession ID: MGI:2445741
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Scamp1
Marker name: secretory carrier membrane protein 1
Marker synonyms: 4930505M11Rik; SCAMP; SCAMP37
Chromosome: 13
Strain of origin: 129S6/SvEvTac
Molecular note: Exons 3 and 4 were replaced with a neomycin selection cassette inserted by homologous recombination. The deleted region encoded amino acids 46 through 144. No protein was detected in homozygous mutant mice by Western blot analysis.