These mice contain loxP sites on either side of exon 23 of the Dicer1 gene. Cre-mediated recombination in the germline leads to developmental arrest. Mutant mice can be used to generate cell/tissue-specific deletions of the endogenous gene for applications in embryonic development, translation, protein processing and miRNA/siRNA regulation of gene expression.
Brian D Harfe, University of FloridaRead More +
These mice contain loxP sites on either side of exon 23. Homozygous mice are viable and fertile with no gross phenotypic or behavioral abnormalities. Expression of the targeted allele is indistinguishable from wild-type despite the frt-flanked neomycin cassette. Cre-mediated recombination (resulting in deletion of exon 23) in the germline leads to developmental arrest at embryonic day 7.5 (E7.5). Tissue specific deletion has been shown to result in loss of microRNA (miRNA) processing. Mutant mice can be used to generate cell/tissue-specific deletions of the endogenous gene for applications in embryonic development, translation, protein processing and miRNA/siRNA regulation of gene expression.
For example, when crossed to a strain expressing Cre recombinase in mesenchyme (see Stock No. 005584), this mutant mouse strain may be useful in studies of limb morphogenesis.
When bred to a strain expressing Cre recombinase in the neural tube (see Stock No. 009107 for example), this mutant mouse strain may be useful in studies of DiGeorge syndrome, miRNA biogenesis and neural crest cell development.
When bred to a strain expressing Cre recombinase in the distal posterior region of E10-E12 embryos (see Stock No. 005622 for example), this mutant mouse strain may be useful in studies of lung epithelial morphogenesis.
When bred to a strain expressing Cre recombinase in Foxp3+ cells from the lymph nodes, spleen and thymus (see Stock No. 23161 for example), this mutant mouse strain may be useful in studies of miRNA and the function of T reg cells.
When bred to a strain expressing Cre recombinase in Foxp3+ regulatory T cells (Foxp3tm4(YFP/cre)Ayr; see Stock No. 016959 for example), this mutant mouse strain may be useful in studies inflammation and the function of T reg cells.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector was used to flank exon 23 (encoding most of the second RNaseIII domain) of the endogenous gene with loxP sites. The vector also contained an frt-flanked neomycin resistance cassette between the exon and downstream loxP site. This construct was electroporated into "129" embryonic stem (ES) cells. Correctly targeted cells were injected into C57BL/6J blastocysts and chimeric mice were bred to C57BL/6J for germline transmission. Heterozygous offspring were bred to generate homozygotes. At some point, homozygotes were bred to mutant Gt(ROSA)26Sor mice on an unknown background. Upon arrival at The Jackson Laboratory, mice on the mixed genetic background were bred to select for the "Dicer-flox" allele and against the Gt(ROSA)26Sor reporter allele, resulting in Stock No. 006001. Mice from that colony were subsequently backcrossed to C57BL/6J for at least five generations to create these mice (Stock No. 006366).
|Allele Name||targeted mutation 1, Brian D Harfe|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||Dcrflox; Dcrfx; Dicer1fl; Dicerflox; Dicerlox; dicerfl|
|Gene Symbol and Name||Dicer1, dicer 1, ribonuclease type III|
|Gene Synonym(s)||1110006F08Rik; 1110006F08Rik; D12Ertd7e; D12Ertd7e; DCR1; DNA segment, Chr 12, ERATO Doi 7, expressed; Dicer; Dicer1, Dcr-1 homolog (Drosophila); Dicer1e; HERNA; K12H4.8-LIKE; MNG1; RIKEN cDNA 1110006F08 gene; RMSE2; mKIAA0928|
|Strain of Origin||129|
|Molecular Note||A targeting vector was designed to insert loxP sites around the exon that encodes most of the second RNaseIII domain as well as an frt-flanked neo within the loxP-encompassed sequence. Cre-mediated removal of the sequence would result in the loss of 90 amino acids, a loss that does not create a downstream frame-shift or an unstable protein.|
|Mutations Made By|| |
Brian D. Harfe and Mike McManus, UFlorida and UCSF
When maintaining a live colony, mutant mice can be bred to C57BL/6J or bred together to create a homozygous colony.
|Please inquire about possible genotypes.|
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
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