Homozygotes carrying the lectin, galactose binding, soluble 3 (Lgals3) knock-out have an impaired acute inflammation response, chondrocyte differentiation during long bone development and myofibroblast activation. This mutant mouse strain may be useful in studies of bone development, endochondral ossification, inflammatory response, and liver fibrosis.
Francoise Poirier, CNRS-Université Paris Diderot
Genetic Background | Generation |
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N5+N3F7
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Lgals3 | lectin, galactose binding, soluble 3 |
Homozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavoiral abnormalities. No gene product (mRNA or protein) is detected by in situ hybridization of tibia bones sections from embryonic day 16.4 mice or by immunohistological staining of fetal skin. Homozygotes have an impaired acute inflammation response. Initial inflammatory infiltrate cell recruitment is normal, but four days after intraperitoneal injection of thioglycollate, mutant mice have a four-fold lower number of recruited granulocytes. Mutant mice have impaired chondrocyte differentiation during long bone development. Fewer hypertrophic chondrocytes but more empty lacunae and condensed chondrocytes are found in the chondrovascular junction. Chondrocytes, cartilage matrix and lacunae are morphologically abnormal. Carbon tetrachloride-induced liver fibrosis results in reduced collagen deposition when compared to wildtype controls. Mutant mice also display defective myofibroblast activation. This mutant mouse strain may be useful in studies of bone development, endochondral ossification, inflammatory response, and liver fibrosis.
This strain was transferred from the Consortium for Functional Glycomics strain collection.
It has been the experience of The Jackson Laboratory that these mice experience a transient period of hairloss between the ages of 2-6 weeks, with full hair growth returning by six weeks of age. Analysis of skin sections reveals a pathology similar to what is seen in C57BL/6 alopecia and movement of these alleles onto the C57BL/6J background may have exacerbated this phenotype.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt 3.7kb of sequence that includes exons 2, 3 and 4. The construct was electroporated into WW6 embryonic stem (ES) cells (derived from 129/Sv, C57BL/6, SJL mixed background mice). Correctly targeted ES cells were injected into outbred MF-1 blastocysts. The resulting chimeric male animals were crossed to 129 female mice. Heterozygotes were crossed to generate homozygotes. The mice were then bred to mice homozygous for a targeted mutation of Lgals1, and he double mutant strain was backcrossed to C57BL/6 for 5 generations. Upon arrival at The Jackson Laboratory, the Lgals1mutation was removed by selective breeding; therefore this strain carries only the Lgals3tm1Poi allele.
Allele Name | targeted mutation 1, Francoise Poirier |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | GaL3-; galectin 3- |
Gene Symbol and Name | Lgals3, lectin, galactose binding, soluble 3 |
Gene Synonym(s) | |
Strain of Origin | STOCK 129/Sv and C57BL/6J and SJL |
Chromosome | 14 |
Molecular Note | 3.7kb of sequence, encompassing exons 2 through 4, was replaced via the insertion of a neomycin selection cassette. Immunostaining of fetal skin showed an absence of protein in homozygous mutant embryos. |
Mutations Made By | Richard Cummings, University of Oklahoma |
When maintaining a live colony, these mice are bred as homozygotes.
When using the galactin3- mouse strain in a publication, please cite the originating article(s) and include JAX stock #006338 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous for Lgals3<tm1Poi> |
Frozen Mouse Embryo | B6.Cg-Lgals3<tm1Poi>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Lgals3<tm1Poi>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Lgals3<tm1Poi>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Lgals3<tm1Poi>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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