B6.FVB(Cg)-Tg(Neurog3-cre)C1Able/J

Stock number: 006333
Product name: Ngn3-Cre
Research areas: Endocrine Deficiency Research, Research Tools

Description

Cre expression in these mice is observed in islets of the adult pancreas, small intestine enteroendocrine cells, endocrine portions of the stomach, all pancreatic endocrine cells, and in some non-endocrine intestinal cells. When bred with a mouse containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked gene in the tissues that normally express neurogenin 3.

Detailed description

Mice hemizygous for the transgenic insert are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Expression of the transgene is directed by a neurogenin 3 promoter. Tissues where Cre recombinase expression is detected include the small intestine (base of intestinal crypts) and fetal pancreatic epithelial cells. Cre activity has been shown in islets of the adult pancreas, small intestine enteroendocrine cells, endocrine portions of the stomach, all pancreatic endocrine cells, and in some non-endocrine intestinal cells. When bred with a mouse containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked gene in the tissues that normally express neurogenin 3.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

A bacterial artificial chromosome (BAC) encoding the mouse neurogenin 3 sequence was modified by the insertion of a cre recombinase gene, preceded by nuclear localization sequence, into exon 1 of the neurogenin 3 gene at the initiator methionine. The BAC was injected into FVB/N embryos which were next implanted into pseudopregnant CD1 females. Founder line C1 was obtained and backcrossed to CD1 mice for 7 generations after which mice were intercrossed. The mice were then backcrossed to C57BL/6J for 5 generations.

Allele

Symbol: Tg(Neurog3-cre)C1Able
Name: transgene insertion C1, Andrew B Leiter
MGI accession ID: MGI:3052639
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Neurog3-cre)C1Able
Marker name: transgene insertion C1, Andrew B Leiter
Chromosome: UN
Strain of origin: Not Specified
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: small intestine (base of intestinal crypts) and fetal pancreatic epithelial cells
Molecular note: The Cre recombinase coding sequence, flanked by a nuclear localization signal and an SV40 polyadenylation site, was inserted just after the translation start site of the neurogenin 3 gene present in mouse BAC RP23-121F10, which also includes approximately 81 kb of 5' and 102 kb of 3' flanking DNA. Immunohistochemical analysis shows the same expression pattern for cre as for neurogenin 3 in adult and embryonic mice bearing this transgene. Cre recombinase activity is seen in cell types of pancreas, stomach, small intestine and colon that express or are descended from cells that express neurogenin 3 during development.
General note: This is one of two independent lines demonstrating identical expression patterns.