NOD.129S6(B6)-Del(3Cd1d2-Cd1d1)1Sbp/SbwJ

Stock number: 006330
Product name: NOD.Cd1d1/Cd1d2^null
Strain synonyms: CD1d-null NOD, NOD.CD1d-/-, NOD.CD1d KO, CD1d-/- NOD, NOD CD1-

Description

These mice carry deletions of Cd1d1 and Cd1d2 and exhibit an accelerated onset and increased incidence of diabetes.

Detailed description

Cd1d1/Cd1d2^tm1Stst homozygous NOD congenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. The lack of CD1d expression on APC in homozygous mice was confirmed by FACS analysis. FACS analysis results also showed when compared to wildtype or heterozygous cohorts that CD1d deficient mice are characterized by a marked loss of NKT cells in spleen, pancreatic lymph nodes and pancreas; while there is an increase in the proportion of conventional T cells expressing CD25 and CD44 indicative of a memory phenotype. Conventional splenic T cells from wildtype or Cd1d deficient mice challenged with autoantigen GAD2 show no difference in proliferation or IL4, IFN-y and IL12 secretion. 50% of 15 week old Cd1d deficient mice are diabetic compared with 0% of wildtype controls and by 32 weeks of age 82% of the CD1d deficient mice are diabetic compared to 56% of the wildtype controls.

This stock is useful in the study of Cd1d function in autoimmunity and peripheral tolerance.

Development

CD1d located on Chromosome 3, 48cM is a nonpolymorphic class Ib protein expressed on the surface of antigen presenting cells. The function of CD1d is to present lipid based antigens to natural killer (NK) T cells. The alleles of interest in this NOD congenic stock are functionally inactivated variants of Cd1d1 and the adjacent Cd1d2 gene. After 11 backcrosses to NOD the size of the 129 derived congenic interval containing the inactivated CD1d gene was <1cM.

Allele

Symbol: Del(3Cd1d2-Cd1d1)1Sbp
Name: deletion, Chr 3, Steven P Balk 1
MGI accession ID: MGI:3611776
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Del(3Cd1d2-Cd1d1)1Sbp
Marker name: deletion, Chr3, Steven P Balk 1
Chromosome: 3
Strain of origin: 129S6/SvEvTac
Molecular note: A genomic region containing Cd1d1 and Cd1d2 genes was replaced with a single neomycin cassette by homologous recombination.