B6Ei.Cg-Nr0b1^tm1.1Lja Tg(Sry)2Ei Chr Y^AKR/EiJ

Stock number: 006305
Product name: DAX W/TG2
Research areas: Developmental Biology Research, Reproductive Biology Research, Research Tools

Development

The Nr0b1^tm1Lja floxed targeted mutation was generated in the mixed 129-derived ES cell line R1 and the resulting mice were bred with 129S1/SvImJ. This floxed allele arrived at The Jackson Laboratory on a primarily 129 background and was backcrossed once to C57BL/6J and then backcrossed repeatedly to C57BL/6JEi in the laboratory of Dr. Eva Eicher. This was then bred with C57BL/6J-Tg(Zp3-cre)93Knw to excise the floxed exon 2. The Tg(Zp3-cre)93Knw was bred away and a mouse heterozygous for the resulting Nr0b1^tm1.1Lja allele was bred with C57BL/6JEi-Tg(Sry)2Ei Chr Y ^AKR/Ei and the resulting offspring were intercrossed generating a strain that could be maintained by breeding females homozygous for the Nr0b1^tm1.1Lja disruption with males hemizygous for Nr0b1^tm1.1Lja/Y^AKR and carrying Tg(Sry)2Ei, which makes them phenotypically fertile males. In 2007 sperm was cryopreserved from males hemizygous for Nr0b1^tm1.1Lja/Y^AKR and Tg(Sry)2Ei.

Allele

Symbol: Tg(Sry)2Ei
Name: transgene insertion 2, Eva M Eicher
MGI accession ID: MGI:3041982
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Sry)2Ei
Marker name: transgene insertion 2, Eva M Eicher
Chromosome: 3
Strain of origin: C57BL/6JEi-Chr Y^AKR
Expressed genes: Sry,sex determining region of Chr Y,mouse, laboratory
Molecular note: This transgene consists of an inbred 129 strain derived 14.6 Kb genomic fragment containing the entire Sry open reading frame and no other genes.

Allele

Symbol: Nr0b1^tm1.1Lja
Name: targeted mutation 1.1, J Larry Jameson
MGI accession ID: MGI:2159321
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Nr0b1
Marker name: nuclear receptor subfamily 0, group B, member 1
Chromosome: X
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: This allele is a derivative of Nr0b1^tm1Lja in which Cre-mediated recombination in vivo under control of a CMV promoter excised exon 2 in the germline. RT-PCR analyses of pituitary, adrenal glands, and gonads did not detect full length transcript in either homozygous female mice or hemizygous male mice.

Allele

Symbol: Sry^AKR/J
Name: sex-determining region of Chr Y- AKR/J
MGI accession ID: MGI:3575651
Generation method: Spontaneous
Marker symbol: Sry
Marker name: sex determining region of Chr Y
Chromosome: Y
Strain of origin: AKR/J
Molecular note: This allele contains all the information necessary and sufficient to initiate normal testis development but is conditionally dysfunctional on the C57BL/6 background.