Eva Eicher, The Jackson Laboratory
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Nr0b1 | nuclear receptor subfamily 0, group B, member 1 |
Allele Type |
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Transgenic (Inserted expressed sequence) |
The Nr0b1tm1Lja floxed targeted mutation was generated in the mixed 129-derived ES cell line R1 and the resulting mice were bred with 129S1/SvImJ. This floxed allele arrived at The Jackson Laboratory on a primarily 129 background and was backcrossed once to C57BL/6J and then backcrossed repeatedly to C57BL/6JEi in the laboratory of Dr. Eva Eicher. This was then bred with C57BL/6J-Tg(Zp3-cre)93Knw to excise the floxed exon 2. The Tg(Zp3-cre)93Knw was bred away and a mouse heterozygous for the resulting Nr0b1tm1.1Lja allele was bred with C57BL/6JEi-Tg(Sry)2Ei Chr Y AKR/Ei and the resulting offspring were intercrossed generating a strain that could be maintained by breeding females homozygous for the Nr0b1tm1.1Lja disruption with males hemizygous for Nr0b1tm1.1Lja/YAKR and carrying Tg(Sry)2Ei, which makes them phenotypically fertile males. In 2007 sperm was cryopreserved from males hemizygous for Nr0b1tm1.1Lja/YAKR and Tg(Sry)2Ei.
Expressed Gene | Sry, sex determining region of Chr Y, mouse, laboratory |
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Site of Expression |
Allele Name | targeted mutation 1.1, J Larry Jameson |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Ahchdelta2; Dax1-; Dax1KO |
Gene Symbol and Name | Nr0b1, nuclear receptor subfamily 0, group B, member 1 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | X |
Molecular Note | This allele is a derivative of Nr0b1tm1Lja in which Cre-mediated recombination in vivo under control of a CMV promoter excised exon 2 in the germline. RT-PCR analyses of pituitary, adrenal glands, and gonads did not detect full length transcript in either homozygous female mice or hemizygous male mice. |
Allele Name | transgene insertion 2, Eva M Eicher |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | Tg(Sry-129)2Ei |
Gene Symbol and Name | Tg(Sry)2Ei, transgene insertion 2, Eva M Eicher |
Gene Synonym(s) | |
Promoter | Sry, sex determining region of Chr Y, mouse, laboratory |
Expressed Gene | Sry, sex determining region of Chr Y, mouse, laboratory |
Strain of Origin | C57BL/6JEi-Chr YAKR |
Chromosome | 3 |
Molecular Note | This transgene consists of an inbred 129 strain derived 14.6 Kb genomic fragment containing the entire Sry open reading frame and no other genes. |
Mutations Made By | Eva Eicher, The Jackson Laboratory |
When using the DAX W/TG2 mouse strain in a publication, please cite the originating article(s) and include JAX stock #006305 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Please Inquire |
Frozen Mouse Embryo | B6Ei.Cg-Nr0b1<tm1.1Lja> Tg(Sry)2Ei Chr Y<AKR>/EiJ | $2595.00 |
Frozen Mouse Embryo | B6Ei.Cg-Nr0b1<tm1.1Lja> Tg(Sry)2Ei Chr Y<AKR>/EiJ | $2595.00 |
Frozen Mouse Embryo | B6Ei.Cg-Nr0b1<tm1.1Lja> Tg(Sry)2Ei Chr Y<AKR>/EiJ | $3373.50 |
Frozen Mouse Embryo | B6Ei.Cg-Nr0b1<tm1.1Lja> Tg(Sry)2Ei Chr Y<AKR>/EiJ | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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