These dopamine transporter IRES-cre (DATIREScre) mutant mice exhibit co-localization of Cre recombinase and endogenous Slc6a3 (solute carrier family 6 (neurotransmitter transporter, dopamine), member 3) gene expression and may be useful in neurobiological studies to facilitate the analysis of gene function in dopaminergic neurons, such as drug addiction or Parkinson's disease.
This strain is discontinued. Please refer to B6.SJL-Slc6a3tm1.1(cre)Bkmn/J Stock No. 006660.
Cristina M Backman, National Institute on Drug Abuse (NIH)
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Recombinase-expressing) | Slc6a3 | solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 |
Mice homozygous for this dopamine transporter IRES-cre (DATIREScre or DAT-cre) mutant allele are viable and fertile. Cre recombinase activity is observed as early as embryonic day 15, and co-localizes with endogenous gene expression in adult dopaminergic cell groups (substantia nigra (SN) and ventral tegmental area (VTA), as well as in the retrorubral field). Lower Cre recombinase activity is detected in adult olfactory bulb glomeruli, mimicking the known lower Slc6a3 (or DAT) expression in this tissue. Although the pattern and intensity of DAT immunostaining in the SN, VTA and striatum do not differ between wildtype and mutant mice, striatum DAT protein levels are moderately reduced (17%) in heterozygotes and significantly reduced (47%) in homozygotes. This decrease in DAT protein levels in homozygous mutant striatum is associated with significantly increased neuropeptide PDyn (but not D1, D2, or PPE) mRNA levels compared to wildtype. Increases in these mRNA levels are not observed in heterozygotes. When these mice are bred with mice containing a loxP-flanked ("floxed") sequence of interest, cre-mediated recombination will result in deletion of the flanked genome in dopaminergic neurons in the cre-positive, homozygous floxed offspring. These mutant mice may facilitate gene function analysis in dopaminergic neurons in neurological studies of, for example, drug addiction or Parkinson's disease.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | Cre recombinase activity is observed as early as embryonic day 15, and co-localizes with endogenous gene expression in adult dopaminergic cell groups (substantia nigra (SN) and ventral tegmental area (VTA), as well as in the retrorubral field). Lesser Cre recombinase activity occurs in adult olfactory bulb glomeruli, mimicking the known lower Slc6a3 (or DAT) expression in this tissue. |
Allele Name | targeted mutation 1.1, Cristina M Backman |
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Allele Type | Targeted (Recombinase-expressing) |
Allele Synonym(s) | DatIRESCre; DatIREScre; DATCre; DAT-IRES-Cre |
Gene Symbol and Name | Slc6a3, solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 |
Gene Synonym(s) | |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Cre recombinase activity is observed as early as embryonic day 15, and co-localizes with endogenous gene expression in adult dopaminergic cell groups (substantia nigra (SN) and ventral tegmental area (VTA), as well as in the retrorubral field). Lesser Cre recombinase activity occurs in adult olfactory bulb glomeruli, mimicking the known lower Slc6a3 (or DAT) expression in this tissue. |
Strain of Origin | Not Specified |
Chromosome | 13 |
General Note | The targeting vector was electroporated into both R1 and C57BL/6 ES cells. |
Molecular Note | An FRT-flanked neo was removed from the locus, leaving cre recombinase cDNA in the 3' UTR to allow bicistronic mRNA expression. |
Mutations Made By | Andreas Tomac, National Institute on Drug Abuse (NIH) |
When maintaining a live colony, heterozygous mice are bred to wildtype siblings or to B6SJLF1/J (Stock No. 100012) F1 hybrids. Homozygous mutant mice are fertile.
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