These transgenic mice harbor a Cre recombinase gene under the control of the rat neuron specific enolase (NSE or Eno2) gene, directing Cre recombinase activity to neurons with expression in many tissue types. These mice may also be useful in studies of spinal muscular atrophy (SMA) .
IMR Colony, The Jackson Laboratory
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing) |
Mice hemizygous for this NSE39-Cre transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These NSE39-Cre mice harbor a transgenic insert consisting of the Cre recombinase gene under the control of the promoter region of the rat neuron specific enolase (NSE or Eno2) gene. As such, Cre recombinase activity is directed to neurons with expression in many tissue types. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked genome.
Specifically, these NSE39-Cre transgenic mice may also be useful in studies of spinal muscular atrophy (SMA) along with mice harboring a conditional (floxed) Smn1 gene (see Stock No. 006138 or Stock No. 006146).
Additional SMA strains expressing cre in striated muscle are available as well (see Stock No. 005936, Stock No. 006139, and Stock No. 006149).
NSE39-Cre transgenic mice are available on different genetic backgrounds (see Stock No. 005938, Stock No. 006297, and Stock No. 006663). In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the NSE39-Cre phenotype could vary from that originally described on a mixed genetic background. We will modify the strain description if necessary as published results become available.
Importation of this model was supported by the Spinal Muscular Atrophy Foundation. Creation and development was supported by the National Institute of Health and Medical Research of France (Inserm) and the Association Française contre les Myopathies (AFM). An additional help was provided by Families of SMA (U.S.A.) and Andrew's Buddies (U.S.A.).
A targeting vector was designed placing a Cre recombinase gene (preceded by the rabbit beta-globin intron and followed by the SV40 polyadenylation signal) under control of the promoter region from the rat neuron specific enolase (NSE or Eno2) gene. This construct was microinjected into (C57BL6 x SJL)F1 embryos and implanted into pseudopregnant foster mothers. Founder 39 was bred to C57BL/6 to generate transgenic mice. At different points while maintaining this strain, transgenic mice were bred with C57BL/6 wild-type mice and/or mice harboring a loxP-flanked exon 7 mutation (Smn1tm1Jme or SMNF7) on a C57BL/6 and "129Sv" mixed background. As the donating investigator reports no germline expression for this transgene, Cre-mediated deletion of the "floxed" exon does not occur in the germline. Thus, offspring contained either the wild-type Smn1 locus or the "floxed" locus; never the Smn1 deletion. Transgenic offspring bearing the wild-type Smn1 locus on this mixed (but predominantly B6;129) background were sent to The Jackson Laboratory by Dr. Judith Melki in 2006. The mice were then backcrossed to FVB/NJ (Stock No. 001800) for 5 generations.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | neurons in many tissue types |
Allele Name | transgene insertion 39, Judith Melki |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | NSE39-Cre; Tg(Nse-cre)1Jme; Tg(Nse-cre)39Jme |
Gene Symbol and Name | Tg(Eno2-cre)39Jme, transgene insertion 39, Judith Melki |
Gene Synonym(s) | |
Promoter | Eno2, enolase 2, gamma, neuronal, rat |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | neurons in many tissue types |
Strain of Origin | (C57BL/6J x SJL)F1 |
Chromosome | UN |
Molecular Note | This transgene expresses Cre recombinase under the control of a rat neuron-specific enolase promoter. |
Mutations Made By | Judith Melki, Genopole, Inserm U798 |
After arriving at The Jackson Laboratory on a mixed background, mutant mice were bred to wild-type FVB/NJ (Stock No. 001800) for 5-10 generations. The resulting backcrossed hemizygotes were maintained thereafter by breeding transgenic mice to either wild-type siblings from the colony or FVB/NJ.
When using the NSE39-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #006297 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Eno2-cre)39Jme |
Frozen Mouse Embryo | FVB.Cg-Tg(Eno2-cre)39Jme/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB.Cg-Tg(Eno2-cre)39Jme/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB.Cg-Tg(Eno2-cre)39Jme/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | FVB.Cg-Tg(Eno2-cre)39Jme/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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