LT/SvEiJ

Stock number: 006252
Research areas: Cancer Research, Reproductive Biology Research

Description

The inbred strain LT carries the spontaneous coat color mutation light (Tyrp1^B-lt). Females exhibit oocyte metaphase I arrest (a result of prolonged spindle assembly checkpoint activity), parthenogenesis, embryonic triploidy and ovarian teratomas.

Detailed description

LT is an inbred strain derived from a C58 mouse, which was outcrossed to BALB/c. LT carries the spontaneous coat color mutation light (B-lt), an arginine to cysteine substitution in tyrosinase-related protein 1 (Tyrp1). LT mice are characterized by a white hair shaft, brown hair tips and pigment dispersion in the iris of the eye. Approximately half of all females develop ovarian teratomas. Poor reproductive success in LT females is attributable to abnormalities in oocyte meiotic cell-cycle (specifically in the spindle assembly checkpoint), spontaneous parthenogenetic activation of oocytes, and the occasional occurrence of digynic triploidy.

Development

This inbred strain began as a spontaneous mutation described as light (B-lt), it was discovered in a C58 colony by Dr. E. C. MacDowell in 1950. The affected light mouse was crossed to BALB and brother x sister mated for 7 generations. The colony was then transferred to Dr. Herman Chase and reached F28 by 1957. Chase sent the mice to Dr. E. S. Russell at The Jackson Laboratory in 1957. From Russell, the colony was transferred to Dr. S. E. Bernstein and in 1971 transferred to Dr. Leroy Stevens at the F75 (Stevens LC, et al., 1974 J:25361). Dr. Eva Eicher took over the colony from Dr. Stevens. It was imported into The Jackson Laboratory Repository in 2006.

Allele

Symbol: Ots1^LT/Sv
Name: LT/Sv
MGI accession ID: MGI:2156627
Generation method: QTL
Marker symbol: Ots1
Marker name: ovarian teratoma susceptibility 1
Chromosome: 6
Strain of origin: LT/Sv
Molecular note: This allele confers ovarian tumor susceptibility compared to C57BL/6J.

Allele

Symbol: Tyrp1^B-lt
Name: light
MGI accession ID: MGI:1855962
Generation method: Spontaneous
Marker symbol: Tyrp1
Marker name: tyrosinase-related protein 1
Chromosome: 4
Strain of origin: C58
Molecular note: A C-to-T transition at coding nucleotide 112 is predicted to result in an arginine to cysteine substitition at codon 38 (p.R38C).