C.Cg-Tg(SFTPC-rtTA)5Jaw/J

Stock number: 006245
Product name: Sp-c-rtTA
Research areas: Developmental Biology Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Mouse/Human Gene Homologs, Research Tools

Description

Expression of rtTA in these transgenic mice is driven by the human SFTPC promoter and detected in lung peripheral epithelial cells from adult mice and in embryos.

Detailed description

Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These transgenic mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the human SFTPC, surfactant, pulmonary-associated protein C, promoter. rtTA activity is detected in lung peripheral epithelial cells from adult mice and in embryos from pregnant females treated with the tetracycline analog, doxycycline (dox). In the latter, rtTA-induced expression of a luciferase reporter under the regulation of a tetracycline-responsive promoter (TRE; tetO) has been detected as early as embryonic day 10.5. When hemizygotes are mated to a second transgenic strain carrying a gene of interest under the regulatory control of a TRE, expression of the target gene in the bitransgenic offspring may be regulated by dox; in the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed. This strain provides a "Tet-On" tool that allows the inducible expression of genes in the developing and adult lung and respiratory epithelium. Please note that some level of rtTA expression may be present in lung tissue from mice untreated with dox.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles frequently are moved to a genetic background different from that on which an allele was first characterized. This is the case for this strain. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

A transgenic construct containing 3.7kb sequence of the human SFTPC, surfactant, pulmonary-associated protein C, gene promoter, the reverse tetracycline regulatable transactivator protein (rtTA) gene, and SV40 polyadenylation signal sequence was injected into mixed C57BL/6 and 129 background embryos. Founder mice were bred to FVB/N animals. Upon arrival at The Jackson Laboratory, these mice were backcrossed to BALB/cJ mice for at least 5 generations

Allele

Symbol: Tg(SFTPC-rtTA)5Jaw
Name: transgene insertion 5, Jeffrey A Whitsett
MGI accession ID: MGI:2445716
Generation method: Transgenic
Attributes: Transactivator
Marker symbol: Tg(SFTPC-rtTA)5Jaw
Marker name: transgene insertion 5, Jeffrey A Whitsett
Chromosome: UN
Strain of origin: Not Specified
Expressed genes: rtTA,reverse tetracycline-controlled transactivator,E. coli
Site of expression: Expresses rtTA in developing and adult lung.
Molecular note: The transgene is composed of 3.7 kb of human SFTPC promoter, a reverse tetracycline responsive transactivator gene (rt-TA), and a 0.45 kb SV40 polyadenylation signal. Transgene expression was confirmed by RT-PCR analysis. The human SFTPC promoter is active in respiratory epithelial cells.