These mice harbor a "knock-out" mutation of the Wnt11 (wingless-related MMTV integration site 11) gene and may be useful in studies of kidney development (including ureteric bud branching morphogenesis) and Wnt superfamily embryogenesis.
Andrew P McMahon, University of Southern California
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Wnt11 | wingless-type MMTV integration site family, member 11 |
Mice heterozygous for this targeted mutation are viable and fertile with normal kidney size and histology. Homozygotes exhibit some embryonic lethality and will die by 2 days post partem. While the cause of death is unclear, these neonates have kidney hypoplasia and reduction of glomeruli. RT-PCR analysis of kidney RNA shows the expected truncated transcript. Homozygotes exhibit ureteric branching morphogenesis defects between embryonic day 11.5-12.5 (T-stage) associated with a reduction in mesenchymal Gdnf expression. These Wnt11 mutant mice may be useful in studies of kidney development, including ureteric bud branching morphogenesis, and Wnt superfamily embryogenesis.
A targeting vector was designed to replace exons 4 and 5 of the endogenous gene with a PGK-neo cassette. This construct was microinjected into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. Chimeric mice were bred to "129 substrain AV3" inbred mice (129X1/SvJ). Heterozygous mice were maintained and expanded on this same genetic background (129X1/SvJ) prior to arrival at The Jackson Laboratory. Upon arrival, these mice were bred to 129X1/SvJ (Stock No. 000691) inbred mice for at least one generation to establish the colony.
Allele Name | targeted mutation 1, Andrew P McMahon |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Wnt11- |
Gene Symbol and Name | Wnt11, wingless-type MMTV integration site family, member 11 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 7 |
Molecular Note | The gene was disrupted by replacement of exons 4 and 5 with a PGK-neo cassette via homologous recombination. RT-PCR analysis of RNA from homozygous mutant animals revealed a truncated transcription product predicted to encode 28 N-terminal amino acids. This mutant protein is expected to be non-functional. |
Mutations Made By | Andrew McMahon, University of Southern California |
When maintaining a live colony, heterozygous mice may be bred together, to wildtype siblings, or to 129X1/SvJ (Stock No. 000691) inbred mice. Homozygotes exhibit some embryonic lethality and will die by 2 days post partem.
When using the Wnt11 knock-out mouse strain in a publication, please cite the originating article(s) and include JAX stock #006239 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-Type for Wnt11<tm1Amc> |
Frozen Mouse Embryo | 129-Wnt11<tm1Amc>/J | $2595.00 |
Frozen Mouse Embryo | 129-Wnt11<tm1Amc>/J | $2595.00 |
Frozen Mouse Embryo | 129-Wnt11<tm1Amc>/J | $3373.50 |
Frozen Mouse Embryo | 129-Wnt11<tm1Amc>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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