These mice harbor a "knock-out" mutation of the Wnt11 (wingless-related MMTV integration site 11) gene and may be useful in studies of kidney development (including ureteric bud branching morphogenesis) and Wnt superfamily embryogenesis.
Andrew P McMahon, University of Southern California
Mice heterozygous for this targeted mutation are viable and fertile with normal kidney size and histology. Homozygotes exhibit some embryonic lethality and will die by 2 days post partem. While the cause of death is unclear, these neonates have kidney hypoplasia and reduction of glomeruli. RT-PCR analysis of kidney RNA shows the expected truncated transcript. Homozygotes exhibit ureteric branching morphogenesis defects between embryonic day 11.5-12.5 (T-stage) associated with a reduction in mesenchymal Gdnf expression. These Wnt11 mutant mice may be useful in studies of kidney development, including ureteric bud branching morphogenesis, and Wnt superfamily embryogenesis.
A targeting vector was designed to replace exons 4 and 5 of the endogenous gene with a PGK-neo cassette. This construct was microinjected into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. Chimeric mice were bred to "129 substrain AV3" inbred mice (129X1/SvJ). Heterozygous mice were maintained and expanded on this same genetic background (129X1/SvJ) prior to arrival at The Jackson Laboratory. Upon arrival, these mice were bred to 129X1/SvJ (Stock No. 000691) inbred mice for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, Andrew P McMahon|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Wnt11, wingless-type MMTV integration site family, member 11|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||The gene was disrupted by replacement of exons 4 and 5 with a PGK-neo cassette via homologous recombination. RT-PCR analysis of RNA from homozygous mutant animals revealed a truncated transcription product predicted to encode 28 N-terminal amino acids. This mutant protein is expected to be non-functional.|
|Mutations Made By|| |
Andrew McMahon, University of Southern California
When maintaining a live colony, heterozygous mice may be bred together, to wildtype siblings, or to 129X1/SvJ (Stock No. 000691) inbred mice. Homozygotes exhibit some embryonic lethality and will die by 2 days post partem.
When using the Wnt11 knock-out mouse strain in a publication, please cite the originating article(s) and include JAX stock #006239 in your Materials and Methods section.