Knock-out of caspase-6 in these mice had no significant effect on lens organelle degradation.
Dr. Richard A. Flavell, Yale University School of Medicine
Homozygous mice are viable and fertile with no gross morphological or behavioral abnormalities. These mutant mice may be useful in studies of mitochondrial events of apoptosis (especially when paired with other executioner caspase mutant models) and lens development.
A targeting vector designed to disrupt the endogenous gene was electroporated into 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric males were bred to C57BL/6 females. Heterozygous mice were backcrossed to C57BL/6 mice for 10 generations before arrival at The Jackson Laboratory.
|Allele Name||targeted mutation 1, Richard A Flavell|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Casp6, caspase 6|
|Strain of Origin||Not Specified|
|Molecular Note||The molecular details are unpublished.|
|Mutations Made By|| |
Dr. Richard Flavell, Yale University School of Medicine
When maintaining a live colony, these mice are bred as homozygotes.
When using the caspase-6 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #006236 in your Materials and Methods section.