Removal of this mouse colony is imminent. If live mice are needed for your studies, it is advised that they be ordered immediately. After removal, the mice will be available from cryorecovery.
Brain development in Casp3-deficient mice shows a variety of hyperplasias and disorganized cell deployment, and ovaries from female homozygotes show aberrant development. These mutant mice may be useful in studies of apoptosis, ovarian follicle and corpus luteum development, and eye and ear developmentDr. Richard A. Flavell, Yale University School of Medicine
Genetic Background | Generation |
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N10+N3F1
|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Casp3 | caspase 3 |
Starting at:
$255.00 Domestic price for female |
333.51 Domestic price for breeder pair |
$2,854.50 Domestic price Cryo Recovery |
On this C57BL/6 congenic background, homozygotes are viable, fertile, and reach adulthood, but females reported display suboptimal mothering instincts. Functional endogenous protein and mRNA are absent from all tissues tested. Homozygous mice are resistant to in vivo cerebral ischemia/reperfusion and in vitro oxygen-glucose deprivation. Ovaries from female homozygotes show aberrant atretic follicles associated with a granulosa cell-intrinsic defect in apoptosis as well as defective corpus luteum regression. Homozygous mice are congenitally deaf with hair cell defects in the Organ of Corti. Optic lens formation/morphology also is abnormal with cataracts at the anterior lens pole. Of note, these mice lack the embryonic/perinatal-lethal brain pathology observed in mutant mice on the 129 and mixed B6;129 genetic backgrounds. These mutant mice may be useful in studies of apoptosis, ovarian follicle and corpus luteum development, and eye and ear development.
A targeting vector was designed to replace the caspase protease-conserved catalytic site of the endogenous gene with a PGK-neo cassette. The construct was electroporated into 129S1/Sv-derived W9.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts, and the resulting chimeric males were bred to C57BL/6 females. Heterozygous mice were backcrossed to C57BL/6 mice for ten generations (see SNP results below) prior to sending females and males to The Jackson Laboratory Repository in 2006. Upon arrival, these females and males were used to establish our living mouse colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be C57BL/6N allele-type. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background, the living colony is on a C57BL/6N genetic background, and the sperm frozen in 2008 is on a C57BL/6N genetic background.
Allele Name | targeted mutation 1, Richard Flavell |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Casp3-; caspase-3-; CPP32- |
Gene Symbol and Name | Casp3, caspase 3 |
Gene Synonym(s) | |
Strain of Origin | 129S1/Sv-Oca2+ Tyr+ Kitl+ |
Chromosome | 8 |
Molecular Note | A genomic fragment containing sequences encoding a conserved motif was replaced with a neomycin selection cassette. RT-PCR analysis on samples derived from brain and liver of homozygous mice demonstrated that no detectable transcript is produced from this allele. Western blot analysis on samples derived from thymocytes of homozygous mice confirmed that no detectable protein was produced. |
Mutations Made By | Dr. Richard Flavell, Yale University School of Medicine |
When maintaining a live colony, heterozygous females are bred with either heterozygous or homozygous males. The donating investigator reports that homozygous females are poor mothers.
When using the CPP32- mouse strain in a publication, please cite the originating article(s) and include JAX stock #006233 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous for Casp3<tm1Flv> |
Frozen Mouse Embryo | B6N.129S1-Casp3<tm1Flv>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6N.129S1-Casp3<tm1Flv>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6N.129S1-Casp3<tm1Flv>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6N.129S1-Casp3<tm1Flv>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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