In these transgenic mice lacZ expression is induced in tissues targeted by toxic compounds such as TCDD.
Dr. Thomas A Gasiewicz, Univ of Rochester, School of Med & Dent
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
Hemizygous mice are viable, fertile, normal in size, and do not display any behavioral abnormalities. Following adult or in utero exposure with xenobiotic ligands (including 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and polychlorinated biphenyls (PCBs)), lacZ expression is induced in tissues targeted by the toxic compounds; for example, embryonic tissues expressing beta-galactosidase following TCDD treatment in utero include hard and soft palates, genital tubercle, certain facial regions, shoulder, and other tissues). These mice may be useful in studies of toxicology, teratogenic and xenobiotic processes, Per-Arnt-Sim transcription factors, cleft-palate, and as a reporter strain to indicate the temporal and spatial context of transcriptionally active aryl hydrocarbon receptors following agonist exposure in vivo.
A transgene was constructed with two dioxin-responsive elements (DRE) and chicken ovalbumin TATA box upstream of a beta-galactosidase (lacZ) gene, all followed by an SV40 intron plus polyadenylation signal. The construct was microinjected into (C57Bl/6 x SJL)F2 fertilized eggs and then implanted into pseudopregnant foster mothers. Founder line DNXT-B (noted numerically as 2 in the strain name) was obtained and bred to C57BL/6 mice for more than 10 generations prior to arrival at The Jackson Laboratory.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | Following adult or in utero exposure with xenobiotic ligands (including TCDD and PCBs), lacZ expression is induced in tissues targeted by the toxic compounds. |
Allele Name | transgene insertion 2, Thomas A Gasiewicz |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | line B |
Gene Symbol and Name | Tg(DRE-lacZ)2Gswz, transgene insertion 2, Thomas A Gasiewicz |
Gene Synonym(s) | |
Promoter | DRE, dioxin-responsive element, |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | Following adult or in utero exposure with xenobiotic ligands (including TCDD and PCBs), lacZ expression is induced in tissues targeted by the toxic compounds. |
Strain of Origin | Not Applicable |
Chromosome | UN |
General Note | Of transgenic lines derived from 7 founder mice that transmitted the transgene, only the present line - designated line B by the authors - exhibited significant hepatic beta-galactosidase induction by TCDD (~20-fold greater than in vehicle-treated mice). Following exposure of the adult or fetal transgenic mouse to xenobiotic ligands, including 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and polychlorinated byphenyls (PCBs), lacZ expression is induced in multiple target tissues. Mice bearing this transgene may be useful for investigations of toxicology, teratogenic and xenobiotic processes, Per-Arnt-Sim transcription factors, and the temporal and spatial context of transcriptionally active aryl hydrocarbon receptor (AhR) following agonist exposure in vivo. |
Molecular Note | The transgene comprises two dioxin-responsive elements (DRE) and the minimal TATA box of the chicken ovalbumin gene upstream of the bacterial lacZ gene, which is followed by an SV40 intron and polyadenylation signal. In the absence of induction by an aryl hydrocarbon receptor (AhR) agonist, weak beta-galactosidase activity is observed only in the embryonic genital tubercle at gestational day E13. |
Mutations Made By | Dr. Thomas Gasiewicz, Univ of Rochester, School of Med & Dent |
When maintaining a live colony, hemizygous mice are interbred or bred to wildtype siblings (or to C57BL/6J).
When using the DRE-lacZ mouse strain in a publication, please cite the originating article(s) and include JAX stock #006229 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(DRE-lacZ)2Gswz |
Frozen Mouse Embryo | B6.Cg-Tg(DRE-lacZ)2Gswz/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(DRE-lacZ)2Gswz/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(DRE-lacZ)2Gswz/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(DRE-lacZ)2Gswz/J Frozen Embryo | $3373.50 |
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