Homozygous mice of this strain harbor the ENU induced Nox3het-3J mutation, are characterized by head tilting, and lack otoconia in the utricle and the saccule of the ear.
Read More +Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Chemically induced (other) (Null/Knockout) | Nox3 | NADPH oxidase 3 |
Homozygotes completely lack otoconia, both in the utricle and the saccule, as early as embryonic day 14 and this persists in the adult. However, the rest of the inner ear appears morphologically normal and hearing is normal. Otoconia appear to be normal in heterozygotes. Homozygotes can be identified by a significant sideways tilting of the head. When dropped 30cm to a soft surface homozygotes fail to land on their feet. They also fail to orient and swim in water, but instead rotate underwater and require rescue.
The head tilt 3 Jackson mutation was generated in an EMS mutagenesis screen. 129S1/SvImJ-derived CJ7 ES cells were treated with ethylmethanesulphonate and injected into C57BL/6J blastocysts to generate chimeras which were bred with C57BL/6J and the head tilt 3 Jackson mutation was identified in subsequent screening progeny. Backcrossing to C57BL/6J continued, resulting in this congenic which reached generation N15 in 2010.
Allele Name | head tilt 3 Jackson |
---|---|
Allele Type | Chemically induced (other) (Null/Knockout) |
Allele Synonym(s) | vst |
Gene Symbol and Name | Nox3, NADPH oxidase 3 |
Gene Synonym(s) | |
Strain of Origin | 129S1/Sv-Oca2+ Tyr+ Kitl+ |
Chromosome | 17 |
Molecular Note | A G to A transition at the -1 position of the spice acceptor site at the intron 1 - exon 2 junction is predicted to result in the abolition of this acceptor site. As a result, PCR suggests two splice variants in the homozygous mutant: one using a cryptic acceptor site in intron 1 and then the canonical donor site of exon 2 (resulting in novel amino-acids and a premature stop codon) and one skipping exon 2 (with the coding region remaining in-frame). No complementation was seen among offspring in crosses between heterozygotes of this allele and homozygotes of head tilt or head tilt 2 Jackson. |
When using the head tilt 3 Jackson mouse strain in a publication, please cite the originating article(s) and include JAX stock #006228 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Nox3<het-3J> |
Frozen Mouse Embryo | B6.129S1-Nox3<het-3J>/GrsrJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S1-Nox3<het-3J>/GrsrJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S1-Nox3<het-3J>/GrsrJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S1-Nox3<het-3J>/GrsrJ Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.