Homozygous Fzd9tm1Uta knock-out mice exhibit abnormal B-cell development, lymphadenopathy, splenomegaly, and accelerated thymic atrophy.
Dr. Uta Francke, Stanford University School of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Fzd9 | frizzled class receptor 9 |
Mice homozygous for this targeted mutation are viable and fertile, with no gross anatomical abnormalities. Endogenous transcripts are absent in skeletal muscle and testis. Homozygotes exhibit abnormal B-cell development, reduced survival, lymphadenopathy secondary to accumulation of plasma cells, splenomegaly, and accelerated thymic atrophy. Mutant mice do not exhibit any obvious features of Williams-Beuren syndrome (WBS). These mice may be useful in studies of hematopoietic/lymphoid development and function (including B-cell and T-cell development), plasma cell homeostasis, and the Wnt/frizzled signaling pathway.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for this strain. It should be noted that the phenotype could vary from that originally published. We will modify the strain description if necessary as published results become available. While the original publication (Ranheim et al 2005 Blood 105:2487-94) describes homozygous mutant mice on a 129 genetic background, the donating investigator reports that no differences in behavioral/cognitive, cardiovascular, or other phenotyping are observed in the C57BL/6J-backcrossed colonies from which The Jackson Laboratory obtained the mice (2006).
A targeting vector was designed to replace the complete open reading frame of the endogenous gene with a neomycin resistance gene. The creator of this strain/donating investigator reports (correcting to the original publication) that this construct was electroporated into 129X1/SvJ-derived JM-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric mice were bred with C57BL/6 females. Mutant mice were backcrossed to C57BL/6J for more than 10 generations prior to arrival at The Jackson Laboratory.
Allele Name | targeted mutation 1, Uta Francke |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Fzd9- |
Gene Symbol and Name | Fzd9, frizzled class receptor 9 |
Gene Synonym(s) | |
Strain of Origin | 129X1/SvJ |
Chromosome | 5 |
Molecular Note | A neomycin resistance cassette replaced the coding exon. Northern blot analysis confirmed the absence of transcription. |
Mutations Made By | Dr. Uta Francke, Stanford University School of Medicine |
When maintaining a live colony, mice are bred as homozygotes.
When using the Fzd9 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #006199 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Fzd9<tm1Uta> |
Frozen Mouse Embryo | B6.129X1-Fzd9<tm1Uta>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129X1-Fzd9<tm1Uta>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129X1-Fzd9<tm1Uta>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129X1-Fzd9<tm1Uta>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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