B6;129S6-Ptgs1^tm1Fun/J

Stock number: 006182
Product name: PTGS1^neo
Research areas: Cardiovascular Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research

Description

These mutant mice have a reduced response to induced ear and paw inflammation, and exhibit impaired platelet aggregation with lengthened bleeding time.

Detailed description

Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Reduced gene product (protein) levels, 10% of wildtype levels, are detected by Western blot analysis of nonstimulated peritoneal macrophages. RT-PCR analysis of stomach and kidney tissue reveals that gene product (mRNA) levels are reduced by 70-73%. This allele is a hypomorph. Mutant mice have a reduced response to arachidonic acid and capsaicin evoked ear inflammation and to carrageenan induced paw edema. Platelet aggregation is impaired as indicated by resistance to arachidonic acid induced thrombosis in vivo, and failure to aggregate in response to arachidonic acid in vitro. Mutant mice exhibit lengthened bleeding time. This mutant mouse strain may be useful in studies related to inflammatory immune response and thrombosis.

Allele

Symbol: Ptgs1^tm1Fun
Name: targeted mutation 1, Colin D Funk
MGI accession ID: MGI:3576237
Generation method: Targeted
Attributes: Hypomorph
Marker symbol: Ptgs1
Marker name: prostaglandin-endoperoxide synthase 1
Chromosome: 2
Strain of origin: 129S6/SvEvTac
Molecular note: A floxed PGK-neo was inserted into intron 10 to create this hypomorphic allele. Western blot showed that protein was dramatically reduced in mutant macrophages to approximately 10% of wild-type levels.