These mutant mice have a reduced response to induced ear and paw inflammation, and exhibit impaired platelet aggregation with lengthened bleeding time.
Colin D. Funk, Queen's University
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Reduced gene product (protein) levels, 10% of wildtype levels, are detected by Western blot analysis of nonstimulated peritoneal macrophages. RT-PCR analysis of stomach and kidney tissue reveals that gene product (mRNA) levels are reduced by 70-73%. This allele is a hypomorph. Mutant mice have a reduced response to arachidonic acid and capsaicin evoked ear inflammation and to carrageenan induced paw edema. Platelet aggregation is impaired as indicated by resistance to arachidonic acid induced thrombosis in vivo, and failure to aggregate in response to arachidonic acid in vitro. Mutant mice exhibit lengthened bleeding time. This mutant mouse strain may be useful in studies related to inflammatory immune response and thrombosis.
|Allele Name||targeted mutation 1, Colin D Funk|
|Allele Type||Targeted (Hypomorph)|
|Allele Synonym(s)||COX-1 KD; PGHS1Neo|
|Gene Symbol and Name||Ptgs1, prostaglandin-endoperoxide synthase 1|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||A floxed PGK-neo was inserted into intron 10 to create this hypomorphic allele. Western blot showed that protein was dramatically reduced in mutant macrophages to approximately 10% of wild-type levels.|
|Mutations Made By|| |
Colin Funk, Queen's University