B6.129-Scel^tm1Hba/J

Stock number: 006156
Product name: sciellin KO
Research areas: Cardiovascular Research, Dermatology Research, Research Tools

Description

While sciellin contributes to the formation of the cornified envelope of the epidermis and stratified squamous epithelia, these sciellin knock-out mice exhibit no structural anomalies.

Detailed description

Homozygous mice are viable and have no overt morphological or developmental abnormalities. The donating investigator reports that fertility may be impaired in homozygous mice (limited number of litters possible). No endogenous gene expression is observed in skin or stratified squamous epithelia. These mutant mice may be useful in dermatological studies, such as cornified envelope formation, organization of the intima, as well as structural distinctions between arteries and veins, and respiratory structure and function.

Development

A targeting vector containing a loxP-flanked neomycin resistance gene was used to replace exons 2 and 3 of the endogenous gene. The construct was electroporated into the "129Svj" embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric mice were bred to C57BL/6J and then backcrossed to the same for 7 generations before arriving at The Jackson Laboratory. This mutation was developed by Incyte Genomic Inc, Palo Alto, California, USA.

Allele

Symbol: Scel^tm1Hba
Name: targeted mutation 1, Howard P Baden
MGI accession ID: MGI:3044133
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Scel
Marker name: sciellin
Chromosome: 14
Strain of origin: 129
Molecular note: Chimeric mice with this allele were generated by Incyte Genomic Inc, Palo Alto CA. Exons 2 and 3 were replaced with a neomycin resistance gene. This resulted in the deletion of the initiation codon found normally in exon 2. Northern and Western blots failed to detect transcript or protein in mutants, respectively.