Mice homozygous for this spontaneous mutation develop severe neural tube defects and die at birth. In it's most severe form, the neural tube fails to initiate Closure 1 (craniorachischichisis). Heterozygotes, predominantly males, often exhibit a looping or kinked tail. This strain may used to research neural tube defects.
Dr. Rivka Rachel, National Cancer Institute
Genetic Background | Generation |
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000654 CBA/CaJ |
|
Allele Type | Gene Symbol | Gene Name |
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Spontaneous (Null/Knockout) | Scrib | scribbled planar cell polarity |
Mice homozygous for this spontaneous mutation develop severe neural tube defects and die at birth. In it's most severe form, the neural tube fails to initiate Closure 1 (craniorachischichisis). Heterozygotes, predominantly males, often exhibit a looping or kinked tail. This strain may used to research neural tube defects.
This spontaneous mutation arose on a mixed NMRI, BALB/c, C57BL/6 transgenic line that had been backcrossed twice to C57BL/6. The circletail phenotype was isolated from the transgenic line and maintained by crossing circletail males with female littermates. The donating investigator maintained the strain by crossing heterozygotes to the inbred strain, CBA/CaJ The backcross generation reached N14 in 2006. The Jackson Laboratory Repository imported this strain in 2007.
Allele Name | circletail |
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Allele Type | Spontaneous (Null/Knockout) |
Allele Synonym(s) | Crc |
Gene Symbol and Name | Scrib, scribbled planar cell polarity |
Gene Synonym(s) | |
Strain of Origin | (NMRI x C57BL/6J)F2 |
Chromosome | 15 |
Molecular Note | A spontaneous mouse mutant that arose in a line carrying a tyrosinase minigene; however, the circletail mutation segregated independently of the transgene. Sequence analysis demonstrated that the coding region contained a single base insertion at codon 947, nucleotide 3182-3 compared with wild-type DNA. The nucleotide insertion is unique to the mutant, and is absent from 16 other mouse strains, including the parental strains on which mutation arose and other unrelated strains. The insertion is predicted to cause a frame shift resulting in a premature termination codon and truncation of the protein to 971 amino acids. Western blot analysis indicates complete absence of the full-length protein in homozygotes and no evidence for a truncated, 100-120 kDa isoform is detected. |
Homozygous mice die at birth.
When using the CBACa.Cg-ScribCrc/RachJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #006130 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-Type for Cg-Scrib<Crc> |
Frozen Mouse Embryo | CBACa.Cg-Scrib<Crc>/RachJ | $2595.00 |
Frozen Mouse Embryo | CBACa.Cg-Scrib<Crc>/RachJ | $2595.00 |
Frozen Mouse Embryo | CBACa.Cg-Scrib<Crc>/RachJ | $3373.50 |
Frozen Mouse Embryo | CBACa.Cg-Scrib<Crc>/RachJ | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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