These Epha2tm1Jrui knock-out mice show impaired ephrin-A1-induced vascular assembly and defective migration, decreased angiogenesis, and failure to activate the Rac1 signaling G protein in response to ephrin-A1.
IMR Colony, The Jackson Laboratory
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Epha2 | Eph receptor A2 |
Mice homozygous for this targeted mutation are viable, fertile, and display no overt developmental or behavioral abnormalities. In mutant mice of a mixed BALB/c, C57BL/6, and 129S6 background, murine pulmonary microvascular endothelial cells (MPMECs) isolated from homozygotes express no endogenous protein. MPMECs from Epha2-deficient mice show impaired ephrin-A1-induced vascular assembly and defective migration both in vitro and in vivo. In addition, MPMECs from homozygous Epha2 mice exhibit decreased angiogenesis and fail to activate Rac1 in response to ephrin-A1 in vivo. Mutant mice on a BALB/c background that were transplanted with metastatic mammary adenocarcinoma cells showed impaired tumor progression, angiogenesis and metastasis to the lung, compared with wildtype littermate controls. These mutant mice may be useful in studies of postnatal angiogenesis (endothelial cell migration, assembly into new tubules, and cytoskeletal regulation), or as a tool to study cancer, especially breast cancer research.
A targeting vector containing the neomycin cassette was used to disrupt exon 5 (at nucleotide 1372, corresponding to amino acid residue 426 in the extracellular domain) of the endogenous Epha2 gene. This construct was microinjected into 129S6/SvEvTac-derived CCE embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Resulting chimeric males were bred to C57BL/6 females for one generation and backcrossed to BALB/c once before being interbred to homozygosity. Mutant null mice were maintained by sibling matings for more than 10 years prior to arrival at The Jackson Laboratory. The mice were then backcrossed on to the BALB/cByJ (Stock No. 001026) background for 5 generations.
Allele Name | targeted mutation 1, Joseph C Ruiz |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Epha2-; Epha2tm1Jinc |
Gene Symbol and Name | Epha2, Eph receptor A2 |
Gene Synonym(s) | |
Strain of Origin | 129S/SvEv-Gpi1c |
Chromosome | 4 |
Molecular Note | A neo cassette was inserted at nucleotide 1372, within the sequence encoding the extracellular domain. Although the resultant transcript putatively encodes a nonfunctional protein truncated at amino acid 426, no protein was detected by Western blot analysis of murine pulmonary microvascular endothelial cells from mutant mice. |
Mutations Made By | Joseph Ruiz, Indiana University School of Medicine |
When maintaining a live colony, these mice are bred as homozygotes.
When using the Epha2- mouse strain in a publication, please cite the originating article(s) and include JAX stock #006070 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Epha2<tm1Jrui> |
Frozen Mouse Embryo | CBy.129S6(B6)-Epha2<tm1Jrui>/J | $2595.00 |
Frozen Mouse Embryo | CBy.129S6(B6)-Epha2<tm1Jrui>/J | $2595.00 |
Frozen Mouse Embryo | CBy.129S6(B6)-Epha2<tm1Jrui>/J | $3373.50 |
Frozen Mouse Embryo | CBy.129S6(B6)-Epha2<tm1Jrui>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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