Mice homozygous for the CAG::H2B-EGFP transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express an H2B-EGFP fusion protein (coding sequence for the human HIST1H2BB gene [histone 1 H2bb] followed C-terminally by Enhanced Green Fluorescent Protein gene [EGFP, BD Biosciences]) under the control of the chicken beta actin promoter/enhancer coupled with the cytomegalovirus (CMV) immediate-early enhancer. Nucleosome and chromatin fluorescence is widespread in the examined tissues, with detectable fluorescence during all phases of mitosis (<a href="https://pubmed.ncbi.nlm.nih.gov/15619330/">Hadjantonakis and Papaioannou 2004 BMC Biotechnol. 4:33 [PMID:15619330]</a>). 
 The donating investigator reports occasional silencing of the transgene when transmitted through the female germline. 
 In 2021, independent researcher data using hemizygous CAG::H2B-EGFP mice shows EGFP fluorescence in several tissues as expected, including pancreas and liver endothelial cells, however there was no EGFP fluorescence in hepatocytes. No immunostaining was attempted to determine if hepatocytes expressed EGFP below fluorescent threshold levels.
 In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

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These CAG::H2B-EGFP transgenic mice express a human histone H2B gene with enhanced green fluorescent protein gene under the control of the chicken beta actin promoter/enhancer (CMV). Nucleosome and chromatin fluorescence is widespread, with detectable fluorescence during all phases of mitosis - see detailed description below. These CAG::H2B-EGFP mice are a fluorescent tool useful for in vivo subcellular high-resolution and 3-dimensional studies of dividing cells.

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