Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size
and do not display any gross physical or behavioral abnormalities. Homozygous null
mice have a perinatal lethal phenotype and die within 24 hours of birth. Occasional
expression of gene product (mRNA) is detected in poorly organized kidney aggregate
tissue of homozygous embryos by in situ hybridization analysis. Expression (mRNA)
is not altered in the central nervous systme and mesonephric mesenchyme.
Homozygous embryos have small kidneys with morphologically undifferentiated renal
mesenchyme. The pituitary glands exhibit abnormal morphology. This mutant mouse
strain may be useful in studies of renal development.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds,
alleles are frequently moved to a genetic background different from that on which an
allele was first characterized. This is the case for the strain above. It should be noted
that the phenotype could vary from that originally described. We will modify the strain
description if necessary as published results become available.
A targeting vector containing a PGK-neo cassette was used to disrupt exon 3. The construct was electroporated into 129S1/Sv-p+ Tyr+ Kitl+ derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to Cre-deleter mice to remove the selection cassette, and then backcrossed to C57BL/6JEiJ (Stock No. 000924) for 20 generations.
|Allele Name||targeted mutation 1, Andrew P McMahon|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Wnt-4-; Wnt4n|
|Gene Symbol and Name||Wnt4, wingless-type MMTV integration site family, member 4|
|Strain of Origin||129S1/Sv-Oca2+ Tyr+ Kitl+|
|Molecular Note||Exon 3, encoding amino acids 106 through 196, was replaced by a PGK-neo cassette. The occasional expression of the targeted locus was observed in poorly developed aggregates in the kidneys of homozygous mutant mice via in situ hybridization. Expression was unaltered in the central nervous system and mesonephric mesenchyme.|
|Mutations Made By|| |
Andrew McMahon, University of Southern California