Homozygous Cbx2tm1Cim (M33-) mice exhibit skeletal malformations and lethality.
Eva Eicher, The Jackson Laboratory
Genetic Background | Generation |
---|---|
000671 DBA/2J |
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Cbx2 | chromobox 2 |
Homozygous Cbx2tm1Cim (M33-) mice show greatly retarded growth, homeotic transformations of the axial skeleton, sternal and limb malformations and a failure to expand in vitro of several cell types including lymphocytes and fibroblasts. In addition, mutant mice show an aggravation of the skeletal malformations when treated to retinoic acid at embryonic day 7.5. Mutant mice develop to term and appear normal, however, most of the M33 mutant mice (90%) die within 4 weeks and the life span does not exceed 6 weeks.
The first 4 exons were replaced via homologous recombination with a floxed neomycin resistance cassette in reverse orientation. This was done in E14 ES cells, derived from 129P2/HsdOla, and ES cells were injected into BALB/c blastocysts. Germ-line transmission was obtained after a backcross between chimeric males and BALB/c females. The strain was maintained by heterozygous matings for more than 2 years. It was imported into The Jackson Laboratory from Dr. Malek Djabali in 1999 and bred to BALB/cByJ then C57BL/6JEi before being backcrossed onto DBA/2J for 9 generations.
Allele Name | targeted mutation 1, Centre d'Immunologie de Marseille Luminy |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | M33 - |
Gene Symbol and Name | Cbx2, chromobox 2 |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 11 |
Molecular Note | A neomycin resistance cassette replaced exons 1-4. RT-PCR analysis confirmed the absence of the transcript in homozygous mice. |
Mutations Made By | Malek Djabali, Universite Paul Sabatier - Toulouse III |
When using the D2-cbx2 mouse strain in a publication, please cite the originating article(s) and include JAX stock #006003 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Cbx2<tm1Cim> |
Frozen Mouse Embryo | D2.129P2(Cg)-Cbx2<tm1Cim>/Ei | $2595.00 |
Frozen Mouse Embryo | D2.129P2(Cg)-Cbx2<tm1Cim>/Ei | $2595.00 |
Frozen Mouse Embryo | D2.129P2(Cg)-Cbx2<tm1Cim>/Ei | $3373.50 |
Frozen Mouse Embryo | D2.129P2(Cg)-Cbx2<tm1Cim>/Ei | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.