Overview

Also Known As:Dicer-floxed

These floxed mutant mice possess loxP sites flanking exon 23 of the Dicer1 gene. This strain may be useful for generating conditional mutations in applications related to embryonic development, translation, protein processing and miRNA/siRNA regulation of gene expression.

Donating Investigator

Brian D Harfe, University of Florida

Genetic overview

Genetic Background	Generation

Dicer1^tm1Bdh

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), No functional change)	Dicer1	dicer 1, ribonuclease type III

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Research Applications

Research Tools
Cell Biology Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

These mice contain loxP sites on either side of exon 23 of the targeted gene. Mice homozygous for this "Dicer-flox" allele are viable and fertile and exhibit no gross phenotypic or behavioral abnormalities. Expression of the targeted allele is indistinguishable from wildtype despite the presence of an frt-flanked neomycin cassette between exon 23 and the 3' loxP site. Cre-mediated recombination (resulting in deletion of exon 23) in the germline leads to developmental arrest at embryonic day 7.5 (E7.5). Tissue specific deletion results in the loss of microRNA (miRNA) processing. Mutant mice can be used to generate cell/tissue-specific deletions of the endogenous gene for applications in embryonic development, translation, protein processing and miRNA/siRNA regulation of gene expression.

For example, when crossed to a strain expressing Cre recombinase in mesenchyme (see Stock No. 005584), this mutant mouse strain may be useful in studies of limb morphogenesis.

When bred to a strain expressing Cre recombinase in the neural tube (see Stock No. 009107 for example), this mutant mouse strain may be useful in studies of DiGeorge syndrome, miRNA biogenesis and neural crest cell development.

When bred to a strain expressing Cre recombinase in the distal posterior region of E10-E12 embryos (see Stock No. 005622 for example), this mutant mouse strain may be useful in studies of lung epithelial morphogenesis.

When bred to a strain expressing Cre recombinase in Foxp3+ cells from the lymph nodes, spleen and thymus (see Stock No. 014579 for example), this mutant mouse strain may be useful in studies miRNA and the function of T reg cells.

When bred to a strain expressing Cre recombinase in Foxp3+ regulatory T cells (Foxp3^{tm4(YFP/cre)Ayr}; see Stock No. 016959 for example), this mutant mouse strain may be useful in studies inflammation and the function of T reg cells.

Development

A targeting vector was used to flank exon 23 (encoding most of the second RNaseIII domain) of the endogenous gene with loxP sites. The vector also contained an frt-flanked neomycin resistance cassette between the exon and downstream loxP site. This construct was electroporated into ?129? embryonic stem (ES) cells. Correctly targeted cells were injected into C57BL/6J blastocysts and chimeric mice were bred to C57BL/6J for germline transmission. Heterozygous offspring were bred to generate homozygotes. At some point, homozygotes were bred to mutant Gt(ROSA)26Sor mice on an unknown background. Upon arrival at The Jackson Laboratory, mutants will be bred to C57BL/6J mice and then wild-type siblings for multiple generations while selecting for the "Dicer-floxed" allele and against the mutant Gt(ROSA)26Sor allele. After this, mice containing only the "Dicer-floxed" allele will be bred together to create a homozygous colony.

Control Suggestions

Approximate Controls

101043 B6129SF1/J

Additional Information

Considerations for Choosing Controls

Selected References

Harfe BD; McManus MT; Mansfield JH; Hornstein E; Tabin CJ. 2005. The RNaseIII enzyme Dicer is required for morphogenesis but not patterning of the vertebrate limb. Proc Natl Acad Sci U S A 102(31):10898-903PubMed: 16040801 MGI: J:100475

View All References

Genetics

Dicer1^tm1Bdh

Allele Symbol: Dicer1^tm1Bdh

Allele Name	targeted mutation 1, Brian D Harfe
Allele Type	Targeted (Conditional ready (e.g. floxed), No functional change)
Allele Synonym(s)	Dcr^flox; Dcr^fx; dicer^fl; Dicer^flox; Dicer^lox; Dicer1^fl
Gene Symbol and Name	Dicer1, dicer 1, ribonuclease type III
Gene Synonym(s)
Strain of Origin	129
Chromosome	12
Molecular Note	A targeting vector was designed to insert loxP sites around the exon that encodes most of the second RNaseIII domain as well as an frt-flanked neo within the loxP-encompassed sequence. Cre-mediated removal of the sequence would result in the loss of 90 amino acids, a loss that does not create a downstream frame-shift or an unstable protein.
Mutations Made By	Brian D. Harfe and Mike McManus, UFlorida and UCSF

Disease/Phenotype

Disease Terms

Model with phenotypic similarity to human disease where etiologies are distinct. Human genes are associated with this disease. Orthologs of these genes do not appear in the mouse genotype(s).

DiGeorge syndrome

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Genetics Research
  - Mutagenesis and Transgenesis: transcriptional activation
  - Mutagenesis and Transgenesis
  - Mutagenesis and Transgenesis: Cre-lox System
- Developmental Biology Research
  - Cre-lox System
- Cre-lox System
  - loxP-flanked Sequences
Cell Biology Research
- Transcriptional Regulation
- Protein Processing

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Dicer1^tm1Bdh/Dicer1^tm1Bdh Tg(Prrx1-cre)1Cjt/0
involves: 129 * C57BL/6J * SJL/J

limbs/digits/tail phenotype

abnormal forelimb morphology
- at E11 the forelimbs are smaller and at E12.5 the forelimb size resembles that of wild-type forelimbs at E11.5
- starting at E10.5 increased cell death is seen in the limb mesoderm

abnormal hindlimb morphology
- starting at E12.5 increased cell death is seen in the limb mesoderm
- the hindlimbs are smaller but not as severely affected as the forelimbs

oligodactyly
- forelimbs show loss of digits and some fusion of digits

skeleton phenotype

abnormal long bone morphology
- the long bones of the arms and legs appear twisted

delayed endochondral bone ossification
- bone formation from cartilage is delayed in the long bones of the limbs

Genotype: Dicer1^tm1Bdh/Dicer1^tm1Bdh Foxp3^{tm4(YFP/icre)Ayr}/Y
involves: 129S1/Sv * 129X1/SvJ

cardiovascular system phenotype

vascular inflammation
- cuffing around blood vessels by inflammatory cells in pancreas, kidney and skeletal muscle is observed

mortality/aging

premature death
- males become ill at 2-3 weeks, exhibiting 100% mortality at <25 days

respiratory system phenotype

lung inflammation
- large areas are infiltrated by inflammatory cells in moribund mice

growth/size/body region phenotype

enlarged spleen

hematopoietic system phenotype

enlarged spleen

immune system phenotype

enlarged lymph nodes

enlarged spleen

liver inflammation
- large areas are infiltrated by inflammatory cells in moribund mice

lung inflammation
- large areas are infiltrated by inflammatory cells in moribund mice

vascular inflammation
- cuffing around blood vessels by inflammatory cells in pancreas, kidney and skeletal muscle is observed

liver/biliary system phenotype

liver inflammation
- large areas are infiltrated by inflammatory cells in moribund mice

Genotype: Dicer1^tm1Bdh/Dicer1^tm1Bdh Foxp3^{tm4(YFP/icre)Ayr}/Foxp3^{tm4(YFP/icre)Ayr}
involves: 129S1/Sv * 129X1/SvJ

cardiovascular system phenotype

vascular inflammation
- cuffing around blood vessels by inflammatory cells in pancreas, kidney and skeletal muscle is observed

mortality/aging

premature death
- females become ill at 2-3 weeks, exhibiting 100% mortality at <25 days

respiratory system phenotype

lung inflammation
- large areas are infiltrated by inflammatory cells in moribund mice

growth/size/body region phenotype

enlarged spleen

hematopoietic system phenotype

enlarged spleen

immune system phenotype

enlarged lymph nodes

enlarged spleen

liver inflammation
- large areas are infiltrated by inflammatory cells in moribund mice

lung inflammation
- large areas are infiltrated by inflammatory cells in moribund mice

vascular inflammation
- cuffing around blood vessels by inflammatory cells in pancreas, kidney and skeletal muscle is observed

liver/biliary system phenotype

liver inflammation
- large areas are infiltrated by inflammatory cells in moribund mice

Genotype: Dicer1^tm1Bdh/Dicer1^tm1Bdh Tg(Foxp3-EGFP/icre)1aJbs/0
involves: 129 * NOD/ShiLt

behavior/neurological phenotype

abnormal behavior
- aging animals display reduced mobility

immune system phenotype

abnormal CD4-positive, alpha-beta T cell physiology
- many effector T cells have high levels of Il4, Il10 and interferon gamma, Ifng whereas <1% of cells from wild-type express Il4, Il10, Il17 or Ifng

abnormal effector T cell morphology
- number of CTLA4-expressing CD4+ Foxp3- effector T cells increase to >50% of splenic and >70% of lymph node CD4+ T cells compared to <10% in wild-type littermates

abnormal lymph node cell ratio
- dramatic increase in lymph node T cells is seen in 4-6 week old mice; however percentage of regulatory T cells is maintained

abnormal regulatory T cell morphology
- T reg cells have a higher percentage of CD103+ T cells and greater number of CD62^lo cells

abnormal regulatory T cell physiology
- CD4+ Foxp3+ T regs have a more prominent activated phenotype (increased expression of CD25, CTLA4 and glucocorticoid-induced TNFR)

abnormal splenic cell ratio
- dramatic increase in splenic T cells is seen in 4-6 week old mice relative to controls

decreased regulatory T cell number
- percentage of regulatory T (T reg) cells in spleen and peripheral blood is reduced to 33% of controls

enlarged lymph nodes
- observed in all aging animals (>5 weeks of age)

enlarged spleen
- observed in all aging animals (>5 weeks of age)

liver inflammation
- tissue displays extensive mononuclear cell infiltration

lung inflammation
- tissue displays extensive mononuclear cell infiltration
- tissue has dense predominantly peribronchovascular infiltrates, composed of CD4+ and Cd8+ T cells

growth/size/body region phenotype

enlarged spleen
- observed in all aging animals (>5 weeks of age)

weight loss
- aging animals display weight loss

liver/biliary system phenotype

liver inflammation
- tissue displays extensive mononuclear cell infiltration

mortality/aging

premature death
- all mice die by 6-8 weeks of age

respiratory system phenotype

lung inflammation
- tissue displays extensive mononuclear cell infiltration
- tissue has dense predominantly peribronchovascular infiltrates, composed of CD4+ and Cd8+ T cells

skeleton phenotype

kyphosis
- seen in aging animals

hematopoietic system phenotype

abnormal CD4-positive, alpha-beta T cell physiology
- many effector T cells have high levels of Il4, Il10 and interferon gamma, Ifng whereas <1% of cells from wild-type express Il4, Il10, Il17 or Ifng

abnormal effector T cell morphology
- number of CTLA4-expressing CD4+ Foxp3- effector T cells increase to >50% of splenic and >70% of lymph node CD4+ T cells compared to <10% in wild-type littermates

abnormal regulatory T cell morphology
- T reg cells have a higher percentage of CD103+ T cells and greater number of CD62^lo cells

abnormal regulatory T cell physiology
- CD4+ Foxp3+ T regs have a more prominent activated phenotype (increased expression of CD25, CTLA4 and glucocorticoid-induced TNFR)

abnormal splenic cell ratio
- dramatic increase in splenic T cells is seen in 4-6 week old mice relative to controls

decreased regulatory T cell number
- percentage of regulatory T (T reg) cells in spleen and peripheral blood is reduced to 33% of controls

enlarged spleen
- observed in all aging animals (>5 weeks of age)

Genotype: Dicer1^tm1Bdh/Dicer1^tm1Bdh H2az2^{Tg(Wnt1-cre)11Rth}/0
involves: 129 * C57BL/6 * CBA/J

cardiovascular system phenotype

abnormal aortic arch development
- discontinuance of the ascending aortic arch with the descending aorta, indicating improper pattering of the aortic arch due to a left fourth aortic arch defect

persistent truncus arteriosis
- the outflow tract does not fully septate into a pulmonary artery and aorta in some cases, resulting in the persistence of a common outflow vessel

ventricular septal defect

hematopoietic system phenotype

athymia
- thymus development is absent

immune system phenotype

athymia
- thymus development is absent

mortality/aging

lethality throughout fetal growth and development, complete penetrance
- die soon after E16.5

nervous system phenotype

abnormal dorsal root ganglion morphology
- loss of neural crest cell derived neuronal tissue from the dorsal root ganglia

abnormal sympathetic ganglion morphology
- loss of neural crest cell derived neuronal tissue from the thoracic sympathetic ganglia

skeleton phenotype

abnormal cartilage morphology
- neural crest cell derived maxillary and mandibular regions of the face and frontonasal process lack cartilaginous tissue, however mesodermally derived cartilage near the base of the skull is present

craniofacial phenotype

abnormal craniofacial morphology
- severe craniofacial defects are seen by E14.5

abnormal first pharyngeal arch morphology
- although pharyngeal arches appear similar to wild-type in emergence, size and shape, expression of Dlx2 and Fgf8 are downregulated in pharyngeal arch 1
- apoptosis is increased in the first pharyngeal arch at E11.5

embryo phenotype

abnormal first pharyngeal arch morphology
- although pharyngeal arches appear similar to wild-type in emergence, size and shape, expression of Dlx2 and Fgf8 are downregulated in pharyngeal arch 1
- apoptosis is increased in the first pharyngeal arch at E11.5

endocrine/exocrine gland phenotype

athymia
- thymus development is absent

Genotype: Dicer1^tm1Bdh/Dicer1⁺ H2az2^{Tg(Wnt1-cre)11Rth}/0
involves: 129 * C57BL/6 * CBA/J

endocrine/exocrine gland phenotype

abnormal thymus development
- thymus development is delayed at E14.5, but by E16.5, the thymus is indistinguishable from wild-type

hematopoietic system phenotype

abnormal thymus development
- thymus development is delayed at E14.5, but by E16.5, the thymus is indistinguishable from wild-type

immune system phenotype

abnormal thymus development
- thymus development is delayed at E14.5, but by E16.5, the thymus is indistinguishable from wild-type

Genotype: Dicer1^tm1Bdh/Dicer1^tm1Bdh Shh^{tm1(EGFP/cre)Cjt}/Shh⁺
involves: 129

mortality/aging

perinatal lethality, complete penetrance
- mice die at birth

respiratory system phenotype

abnormal lung development
- at E11.75, expression of Fgf10 (a key gene involved in lung development) is already up-regulated and expanded in the mesenchyme of mutant lungs
- at E12.5, fewer branches are observed and the distal tips of the newly formed branches appear dilated
- defective branching morphogenesis occurs prior to the increased cell death seen in the distal epithelium at E13.0
- the number of dilated branching tips observed at E12.5 corresponds to the number of large, fluid-filled sacs seen at E15.5

abnormal lung epithelium morphology
- at E12.25, increased epithelial cell death is ectopically observed in the secondary bronchi of mutant lungs in addition to the normal pattern of cell death seen in the trachea and primary bronchi
- at E12.5 and E12.75, intense epithelial cell death is prolonged in the mutant trachea and bronchi, unlike in control and wild type lungs
- at E15.5, mutant lung epithelial cells form large, fluid-filled sacs within each lobe, indicating a severe branching defect
- at E15.5, the mutant epithelium is often detached from the mesenchyme, unlike in control lungs
- by E13.0, aberrant cell death is observed in the entire mutant lung epithelium, including the distal branching region, but not in the mesenchyme

small lung lobe
- after E13.5, each mutant lung lobe is proportionally smaller than that in control lungs
- Normal - however, each lobe maintains a normal shape, indicating a normal lobation pattern

Genotype: Dicer1^tm1Bdh/Dicer1^tm1Bdh Edil3^{Tg(Sox2-cre)1Amc}/Edil3⁺
involves: 129 * C57BL/6 * CBA

embryo phenotype

spina bifida
- observed at E8.5

nervous system phenotype

spina bifida
- observed at E8.5

Genotype: Dicer1^tm1Bdh/Dicer1^tm1Bdh Tg(Chx10-EGFP/cre,-ALPP)2Clc/0
involves: 129 * C57BL/6 * SJL

nervous system phenotype

abnormal retinal photoreceptor morphology
- mice show the formation of photoreceptor rosettes at P16 (circular structures comprising photoreceptors only that are oriented toward an internal lumen with photoreceptor outer segments protruding inward) that increase in number from P16 and P45 and decrease in size and eventually disappear by 3 months of age when degeneration of photoreceptors begins
- rosettes are scattered along the retinal outer surface and are composed of photoreceptors and their synaptic terminals

retinal photoreceptor degeneration
- photoreceptors with rosettes degenerate as mice reach 3 months of age

vision/eye phenotype

abnormal retinal layer morphology
- Normal - however, no major defects in neuroepithelial cell polarity or in structure of adherens junctions are seen in immature retinas at P2
- mice show progressive alteration and remodeling of the laminar retinal structure from P16 to P45

abnormal retinal photoreceptor morphology
- mice show the formation of photoreceptor rosettes at P16 (circular structures comprising photoreceptors only that are oriented toward an internal lumen with photoreceptor outer segments protruding inward) that increase in number from P16 and P45 and decrease in size and eventually disappear by 3 months of age when degeneration of photoreceptors begins
- rosettes are scattered along the retinal outer surface and are composed of photoreceptors and their synaptic terminals

decreased a wave amplitude
- scotopic a wave amplitude is diminished at 1, 3, and 5 months of age

decreased b wave amplitude
- scotopic and photopic b wave amplitudes are diminished

disorganized retinal layers
- formation of photoreceptor rosettes at P16 progresses to more general cellular disorganization

retinal degeneration
- widespread degeneration of retinal cell types with age

retinal gliosis
- Muller cells become hypertrophic with radial processes increasing in size and GFAP reactivity increasing at later stages during when loss of retinal cells is occurring, indicating glial activation

retinal photoreceptor degeneration
- photoreceptors with rosettes degenerate as mice reach 3 months of age

thin retinal outer nuclear layer
- the outer nuclear layer becomes progressively thinner; it is thinner in the peripheral compared with the central retina, indicating a periphery-to-central degenerative gradient

Genotype: Dicer1^tm1Bdh/Dicer1⁺ Tg(Chx10-EGFP/cre,-ALPP)2Clc/0
involves: 129 * C57BL/6 * SJL

mammalian phenotype

vision/eye phenotype
- Normal - no morphological abnormalities are seen in the retina

vision/eye phenotype

decreased a wave amplitude
- scotopic a wave amplitude is reduced at 1, 3, and 5 months of age

decreased b wave amplitude
- scotopic and photopic b wave amplitudes are reduced at 1, 3, and 5 months of age

Genotype: Dicer1^tm1Bdh/Dicer1^tm1Bdh Tg(CAG-cre/Esr1)5Amc/0
involves: 129 C57BL/6 * CBA

muscle phenotype

impaired skeletal muscle regeneration
- lower mean of regenerating myofiber cross-sectional area of tibialis anterior (TA) muscle 14 days after intramuscular injection of cardiotoxin (CTX), after induction of knockout with tamoxifen
- Normal - normal number of satellite cells and normal primary myoblast differentiation and viability after induction of knockout with tamoxifen

References

Harfe BD; McManus MT; Mansfield JH; Hornstein E; Tabin CJ. 2005. The RNaseIII enzyme Dicer is required for morphogenesis but not patterning of the vertebrate limb. Proc Natl Acad Sci U S A 102(31):10898-903PubMed: 16040801 MGI: J:100475

Additional - Dicer1^tm1Bdh related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Dicer1
- Genotyping resources and troubleshooting
Breeding Considerations

Upon arrival at The Jackson Laboratory, mutants will be bred to B6129F1 (Stock No. 101043) mice for multiple generations while selecting for the "floxed" allele and against the mutant Gt(ROSA)26Sor allele. After this, mice containing only the "floxed" allele will be bred together to create a homozygous colony.
- Additional Breeding and Husbandry Support
Citation
When using the Dicer-floxed mouse strain in a publication, please cite the originating article(s) and include JAX stock #006001 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous for Dicer1<tm1Bdh>

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Also Known As:Dicer-floxed

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Selected References

Dicer1tm1Bdh

Allele Symbol: Dicer1tm1Bdh

Disease Terms

Model with phenotypic similarity to human disease where etiologies are distinct. Human genes are associated with this disease. Orthologs of these genes do not appear in the mouse genotype(s).

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Dicer1tm1Bdh/Dicer1tm1Bdh Tg(Prrx1-cre)1Cjt/0involves: 129 * C57BL/6J * SJL/J

limbs/digits/tail phenotype

skeleton phenotype

Genotype: Dicer1tm1Bdh/Dicer1tm1Bdh Foxp3tm4(YFP/icre)Ayr/Yinvolves: 129S1/Sv * 129X1/SvJ

cardiovascular system phenotype

mortality/aging

respiratory system phenotype

growth/size/body region phenotype

hematopoietic system phenotype

immune system phenotype

liver/biliary system phenotype

Genotype: Dicer1tm1Bdh/Dicer1tm1Bdh Foxp3tm4(YFP/icre)Ayr/Foxp3tm4(YFP/icre)Ayrinvolves: 129S1/Sv * 129X1/SvJ

cardiovascular system phenotype

mortality/aging

respiratory system phenotype

growth/size/body region phenotype

hematopoietic system phenotype

immune system phenotype

liver/biliary system phenotype

Genotype: Dicer1tm1Bdh/Dicer1tm1Bdh Tg(Foxp3-EGFP/icre)1aJbs/0involves: 129 * NOD/ShiLt

behavior/neurological phenotype

immune system phenotype

growth/size/body region phenotype

liver/biliary system phenotype

mortality/aging

respiratory system phenotype

skeleton phenotype

hematopoietic system phenotype

Genotype: Dicer1tm1Bdh/Dicer1tm1Bdh H2az2Tg(Wnt1-cre)11Rth/0involves: 129 * C57BL/6 * CBA/J

cardiovascular system phenotype

hematopoietic system phenotype

immune system phenotype

mortality/aging

nervous system phenotype

skeleton phenotype

craniofacial phenotype

embryo phenotype

endocrine/exocrine gland phenotype

Genotype: Dicer1tm1Bdh/Dicer1+ H2az2Tg(Wnt1-cre)11Rth/0involves: 129 * C57BL/6 * CBA/J

endocrine/exocrine gland phenotype

hematopoietic system phenotype

immune system phenotype

Genotype: Dicer1tm1Bdh/Dicer1tm1Bdh Shhtm1(EGFP/cre)Cjt/Shh+involves: 129

mortality/aging

respiratory system phenotype

Genotype: Dicer1tm1Bdh/Dicer1tm1Bdh Edil3Tg(Sox2-cre)1Amc/Edil3+involves: 129 * C57BL/6 * CBA

embryo phenotype

nervous system phenotype

Genotype: Dicer1tm1Bdh/Dicer1tm1Bdh Tg(Chx10-EGFP/cre,-ALPP)2Clc/0involves: 129 * C57BL/6 * SJL

nervous system phenotype

vision/eye phenotype

Genotype: Dicer1tm1Bdh/Dicer1+ Tg(Chx10-EGFP/cre,-ALPP)2Clc/0involves: 129 * C57BL/6 * SJL

mammalian phenotype

vision/eye phenotype

Genotype: Dicer1tm1Bdh/Dicer1tm1Bdh Tg(CAG-cre/Esr1*)5Amc/0involves: 129 * C57BL/6 * CBA

muscle phenotype

References

Additional - Dicer1tm1Bdh related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Dicer1^tm1Bdh

Allele Symbol: Dicer1^tm1Bdh

Genotype: Dicer1^tm1Bdh/Dicer1^tm1Bdh Tg(Prrx1-cre)1Cjt/0
involves: 129 * C57BL/6J * SJL/J

Genotype: Dicer1^tm1Bdh/Dicer1^tm1Bdh Foxp3^{tm4(YFP/icre)Ayr}/Y
involves: 129S1/Sv * 129X1/SvJ

Genotype: Dicer1^tm1Bdh/Dicer1^tm1Bdh Foxp3^{tm4(YFP/icre)Ayr}/Foxp3^{tm4(YFP/icre)Ayr}
involves: 129S1/Sv * 129X1/SvJ

Genotype: Dicer1^tm1Bdh/Dicer1^tm1Bdh Tg(Foxp3-EGFP/icre)1aJbs/0
involves: 129 * NOD/ShiLt

Genotype: Dicer1^tm1Bdh/Dicer1^tm1Bdh H2az2^{Tg(Wnt1-cre)11Rth}/0
involves: 129 * C57BL/6 * CBA/J

Genotype: Dicer1^tm1Bdh/Dicer1⁺ H2az2^{Tg(Wnt1-cre)11Rth}/0
involves: 129 * C57BL/6 * CBA/J

Genotype: Dicer1^tm1Bdh/Dicer1^tm1Bdh Shh^{tm1(EGFP/cre)Cjt}/Shh⁺
involves: 129

Genotype: Dicer1^tm1Bdh/Dicer1^tm1Bdh Edil3^{Tg(Sox2-cre)1Amc}/Edil3⁺
involves: 129 * C57BL/6 * CBA

Genotype: Dicer1^tm1Bdh/Dicer1^tm1Bdh Tg(Chx10-EGFP/cre,-ALPP)2Clc/0
involves: 129 * C57BL/6 * SJL

Genotype: Dicer1^tm1Bdh/Dicer1⁺ Tg(Chx10-EGFP/cre,-ALPP)2Clc/0
involves: 129 * C57BL/6 * SJL

Genotype: Dicer1^tm1Bdh/Dicer1^tm1Bdh Tg(CAG-cre/Esr1)5Amc/0
involves: 129 C57BL/6 * CBA

Additional - Dicer1^tm1Bdh related