JAX Mouse Strain Datasheet - 005992

STOCK Efna2^tm1Jgf Efna5^tm1Ddmo/J

Stock No:: 005992

Targeted Mutation

Cryo Recovery

Overview

These ephrin A2/ephrin A5 double mutant mice may be useful in studies of retinocollicular mapping, axon topography, neuron projection, and modality-specific compartmentalization of visual, auditory, and somatosensory thalamic relay pathways.

Donating Investigator

David Feldhiem, University of California at Santa Cruz

Genetic overview

Genetic Background	Generation

Efna2^tm1Jgf

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Efna2	ephrin A2

Efna5^tm1Ddmo

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Efna5	ephrin A5

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Research Applications

Developmental Biology Research
Neurobiology Research
Research Tools
Sensorineural Research

View All Research Applications

Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

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Details

Detailed Description

Mice homozygous for both targeted mutations are viable, fertile, and normal in size. While the donating investigator reports that 10-20% of females homozygous at both loci neglect their litters, no such neglect is reported in the colonies at The Jackson Laboratory (Aug 2009). Double homozygous mice have no endogenous protein expression in inferior colliculus (IC) or superior colliculus (SC), and thus lack the concentration gradient created by the endogenous proteins across the midbrain in wildtype mice. Temporal and nasal retinal axon termination is severely altered: multiple ectopic aborizations in the SC indicate abnormalities in both anteroposterior and dorsoventral topography. Following surgical ablation of portions of the midbrain (including IC and SC), cross-modal innervation by retinal neurons is greater in double homozygous mutants compared to wildtype. Mice heterozygous at both loci are reported to have greater reproductive performance compared to double homozygous mice. Further, double heterozygotes have temporal (but not nasal) retinal axon aborization in the SC with diminished frequency and severity. These ephrin A2/ephrin A5 double mutant mice may be useful in studies of retinocollicular mapping, axon topography, neuron projection, and modality-specific compartmentalization of visual, auditory, and somatosensory thalamic relay pathways.

Development

These mice harbor two targeted mutations; ephrin A2 and ephrin A5. For the ephrin A2 targeted mutation, a targeting vector was designed to insert a neomycin cassette in exon 2 after amino acid 66 (and just upstream of a cysteine repeat motif conserved throughout the ephrin family) of the Efna2 gene. The construct was electroporated into 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. Chimeric mice were bred with 129/SvEv mice. Mutant offspring were crossed to create homozygous mutant mice (Efna2^-/-). The ephrin A5 targeted mutation was independently created by replacing the portion of Efna5 exon 2 encoding amino acids 42-129 with a PGK-neo cassette. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 ES cells. Chimeric mice were bred with C57BL/6 mice. Mutant offspring were crossed to Swiss Webster mice and then made homozygous for this mutation (Efna5^-/-). Double mutant mice were obtained by crossing Efna2^-/- in a 129/SvEv background with a Efna5^-/- in a mixed background (Swiss-Webster, C57BL/6, 129). Upon arrival at The Jackson Laboratory, double mutant mice were bred with C57BL/6J for at least one generation to establish the colony.

Control Suggestions

None Available

Additional Information

Considerations for Choosing Controls

Selected References

Feldheim DA; Kim YI; Bergemann AD; Frisen J; Barbacid M; Flanagan JG. 2000. Genetic analysis of ephrin-A2 and ephrin-A5 shows their requirement in multiple aspects of retinocollicular mapping [see comments] Neuron 25(3):563-74. PubMed: 10774725 MGI: J:61499

View All References

Genetics

Efna2^tm1Jgf

Allele Symbol: Efna2^tm1Jgf

Allele Name	targeted mutation 1, John G Flanagan
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	A2^-; ephrin-A2^-
Gene Symbol and Name	Efna2, ephrin A2
Gene Synonym(s)	Cek7-L; ELF-1; EPLG6; Elf-1; Ephrin-A2; Epl6; Epl6; HEK7-L; LERK-6; LERK6; eph-related receptor tyrosine kinase ligand 6; ephrin A6
Strain of Origin	129S6/SvEvTac
Chromosome	10
Molecular Note	A neomycin selection cassette, containing stop codons in all three reading frames, was inserted after the sequence encoding amino acid 66. The insertion was upstream of a conserved cysteine residue motif. RT-PCR analysis of midbrain RNA showed an absence of transcript in homozygous mutant embryos.

Efna5^tm1Ddmo

Allele Symbol: Efna5^tm1Ddmo

Allele Name	targeted mutation 1, Dennis D M O'Leary
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	A5^-; eA5KO; ephrin-A5^-
Gene Symbol and Name	Efna5, ephrin A5
Gene Synonym(s)	AF1; AL-1; AV158822; EFL-5; EFL5; EPLG7; Ephrin-A5; Epl7; Epl7; GLC1M; LERK-7; LERK7; Lerk7; RAGS; eph-related receptor tyrosine kinase ligand 7; expressed sequence AV158822
Strain of Origin	(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Chromosome	17
Molecular Note	A neomycin selection cassette replaced sequence encoding amino acids 42 through 129 as well as a 5' splice acceptor. RT-PCR analysis showed an absence of transcript in homozygous mutant mice.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Developmental Biology Research
- Internal/Organ Defects
  - brain
- Neurodevelopmental Defects
Neurobiology Research
- Neurodevelopmental Defects
Research Tools
- Neurobiology Research
- Sensorineural Research
Sensorineural Research
- Eye Defects

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Efna2^tm1Jgf/Efna2⁺ Efna5^tm1Ddmo/Efna5⁺
involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ

nervous system phenotype

abnormal sensory neuron innervation pattern
- single ectopic arborizations of temporal axons from the retina but normal nasal arborizations

Genotype: Efna2^tm1Jgf/Efna2^tm1Jgf Efna5^tm1Ddmo/Efna5^tm1Ddmo
involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ

nervous system phenotype

abnormal sensory neuron innervation pattern
- ectopic arborization of both retinal temporal axons and nasal axons are more sever than in singly homozygous mice
- normal arborization sometimes lost
- usually multiple ectopic arborizations are seen

References

Feldheim DA; Kim YI; Bergemann AD; Frisen J; Barbacid M; Flanagan JG. 2000. Genetic analysis of ephrin-A2 and ephrin-A5 shows their requirement in multiple aspects of retinocollicular mapping [see comments] Neuron 25(3):563-74. PubMed: 10774725 MGI: J:61499

Additional - Efna2^tm1Jgf related

Abdul-Latif ML; Salazar JA; Marshak S; Dinh ML; Cramer KS. 2015. Ephrin-A2 and ephrin-A5 guide contralateral targeting but not topographic mapping of ventral cochlear nucleus axons. Neural Dev 10(1):27. PubMed: 26666565 MGI: J:227114
Bonanomi D; Chivatakarn O; Bai G; Abdesselem H; Lettieri K; Marquardt T; Pierchala BA; Pfaff SL. 2012. Ret Is a Multifunctional Coreceptor that Integrates Diffusible- and Contact-Axon Guidance Signals. Cell 148(3):568-82. PubMed: 22304922 MGI: J:180801
Brennaman LH; Zhang X; Guan H; Triplett JW; Brown A; Demyanenko GP; Manis PB; Landmesser L; Maness PF. 2013. Polysialylated NCAM and ephrinA/EphA regulate synaptic development of GABAergic interneurons in prefrontal cortex. Cereb Cortex 23(1):162-77. PubMed: 22275477 MGI: J:204427
Cang J; Kaneko M; Yamada J; Woods G; Stryker MP; Feldheim DA. 2005. Ephrin-as guide the formation of functional maps in the visual cortex. Neuron 48(4):577-89. PubMed: 16301175 MGI: J:107593
Cang J; Niell CM; Liu X; Pfeiffenberger C; Feldheim DA; Stryker MP. 2008. Selective disruption of one Cartesian axis of cortical maps and receptive fields by deficiency in ephrin-As and structured activity. Neuron 57(4):511-23. PubMed: 18304481 MGI: J:132879
Cang J; Wang L; Stryker MP; Feldheim DA. 2008. Roles of ephrin-as and structured activity in the development of functional maps in the superior colliculus. J Neurosci 28(43):11015-23. PubMed: 18945909 MGI: J:143794
Chadaram SR; Laskowski MB; Madison RD. 2007. Topographic specificity within membranes of a single muscle detected in vitro. J Neurosci 27(51):13938-48. PubMed: 18094231 MGI: J:141637
Ellsworth CA; Lyckman AW; Feldheim DA; Flanagan JG; Sur M. 2005. Ephrin-A2 and -A5 influence patterning of normal and novel retinal projections to the thalamus: conserved mapping mechanisms in visual and auditory thalamic targets. J Comp Neurol 488(2):140-51. PubMed: 15924339 MGI: J:99706
Feng G; Laskowski MB; Feldheim DA; Wang H; Lewis R; Frisen J; Flanagan JG; Sanes JR. 2000. Roles for ephrins in positionally selective synaptogenesis between motor neurons and muscle fibers. Neuron 25(2):295-306. PubMed: 10719886 MGI: J:60774
Haustead DJ; Lukehurst SS; Clutton GT; Bartlett CA; Dunlop SA; Arrese CA; Sherrard RM; Rodger J. 2008. Functional topography and integration of the contralateral and ipsilateral retinocollicular projections of ephrin-A-/- mice. J Neurosci 28(29):7376-86. PubMed: 18632942 MGI: J:139255
Hjorth JJ; Sterratt DC; Cutts CS; Willshaw DJ; Eglen SJ. 2015. Quantitative assessment of computational models for retinotopic map formation. Dev Neurobiol 75(6):641-66. PubMed: 25367067 MGI: J:242263
Holmberg J; Armulik A; Senti KA; Edoff K; Spalding K; Momma S; Cassidy R; Flanagan JG; Frisen J. 2005. Ephrin-A2 reverse signaling negatively regulates neural progenitor proliferation and neurogenesis. Genes Dev 19(4):462-71. PubMed: 15713841 MGI: J:96025
Jiao JW; Feldheim DA; Chen DF. 2008. Ephrins as negative regulators of adult neurogenesis in diverse regions of the central nervous system. Proc Natl Acad Sci U S A 105(25):8778-83. PubMed: 18562299 MGI: J:137200
Lyckman AW; Jhaveri S; Feldheim DA; Vanderhaeghen P; Flanagan JG; Sur M. 2001. Enhanced plasticity of retinothalamic projections in an ephrin-A2/A5 double mutant. J Neurosci 21(19):7684-90. PubMed: 11567058 MGI: J:71649
Makowiecki K; Hammond G; Rodger J. 2012. Different levels of food restriction reveal genotype-specific differences in learning a visual discrimination task. PLoS One 7(11):e48703. PubMed: 23144936 MGI: J:195031
Noh H; Lee H; Park E; Park S. 2016. Proper closure of the optic fissure requires ephrin A5-EphB2-JNK signaling. Development 143(3):461-72. PubMed: 26839344 MGI: J:240082
Park E; Kim Y; Noh H; Lee H; Yoo S; Park S. 2013. EphA/ephrin-A signaling is critically involved in region-specific apoptosis during early brain development. Cell Death Differ 20(1):169-80. PubMed: 22976838 MGI: J:205629
Pfeiffenberger C; Cutforth T; Woods G; Yamada J; Renteria RC; Copenhagen DR; Flanagan JG; Feldheim DA. 2005. Ephrin-As and neural activity are required for eye-specific patterning during retinogeniculate mapping. Nat Neurosci 8(8):1022-7. PubMed: 16025107 MGI: J:101436
Pfeiffenberger C; Yamada J; Feldheim DA. 2006. Ephrin-As and patterned retinal activity act together in the development of topographic maps in the primary visual system. J Neurosci 26(50):12873-84. PubMed: 17167078 MGI: J:116767
Suetterlin P; Drescher U. 2014. Target-independent ephrina/EphA-mediated axon-axon repulsion as a novel element in retinocollicular mapping. Neuron 84(4):740-52. PubMed: 25451192 MGI: J:223076
Ting MC; Wu NL; Roybal PG; Sun J; Liu L; Yen Y; Maxson RE Jr. 2009. EphA4 as an effector of Twist1 in the guidance of osteogenic precursor cells during calvarial bone growth and in craniosynostosis. Development 136(5):855-64. PubMed: 19201948 MGI: J:146459
Torii M; Hashimoto-Torii K; Levitt P; Rakic P. 2009. Integration of neuronal clones in the radial cortical columns by EphA and ephrin-A signalling. Nature 461(7263):524-8. PubMed: 19759535 MGI: J:153504
Triplett JW; Phan A; Yamada J; Feldheim DA. 2012. Alignment of multimodal sensory input in the superior colliculus through a gradient-matching mechanism. J Neurosci 32(15):5264-71. PubMed: 22496572 MGI: J:184449
Wang L; Klein R; Zheng B; Marquardt T. 2011. Anatomical Coupling of Sensory and Motor Nerve Trajectory via Axon Tracking. Neuron 71(2):263-77. PubMed: 21791286 MGI: J:174684
Wilks TA; Rodger J; Harvey AR. 2010. A role for ephrin-As in maintaining topographic organization in register across interconnected central visual pathways. Eur J Neurosci 31(4):613-22. PubMed: 20384808 MGI: J:159859
Wurzman R; Forcelli PA; Griffey CJ; Kromer LF. 2014. Repetitive grooming and sensorimotor abnormalities in an ephrin-A knockout model for Autism Spectrum Disorders. Behav Brain Res 278C:115-128. PubMed: 25281279 MGI: J:216970
Yu X; Wang G; Gilmore A; Yee AX; Li X; Xu T; Smith SJ; Chen L; Zuo Y. 2013. Accelerated experience-dependent pruning of cortical synapses in ephrin-A2 knockout mice. Neuron 80(1):64-71. PubMed: 24094103 MGI: J:201801

Additional - Efna5^tm1Ddmo related

Abdul-Latif ML; Salazar JA; Marshak S; Dinh ML; Cramer KS. 2015. Ephrin-A2 and ephrin-A5 guide contralateral targeting but not topographic mapping of ventral cochlear nucleus axons. Neural Dev 10(1):27. PubMed: 26666565 MGI: J:227114
Bonanomi D; Chivatakarn O; Bai G; Abdesselem H; Lettieri K; Marquardt T; Pierchala BA; Pfaff SL. 2012. Ret Is a Multifunctional Coreceptor that Integrates Diffusible- and Contact-Axon Guidance Signals. Cell 148(3):568-82. PubMed: 22304922 MGI: J:180801
Brennaman LH; Zhang X; Guan H; Triplett JW; Brown A; Demyanenko GP; Manis PB; Landmesser L; Maness PF. 2013. Polysialylated NCAM and ephrinA/EphA regulate synaptic development of GABAergic interneurons in prefrontal cortex. Cereb Cortex 23(1):162-77. PubMed: 22275477 MGI: J:204427
Buensuceso AV; Son AI; Zhou R; Paquet M; Withers BM; Deroo BJ. 2016. Ephrin-A5 Is Required for Optimal Fertility and a Complete Ovulatory Response to Gonadotropins in the Female Mouse. Endocrinology 157(2):942-55. PubMed: 26672804 MGI: J:233848
Cang J; Kaneko M; Yamada J; Woods G; Stryker MP; Feldheim DA. 2005. Ephrin-as guide the formation of functional maps in the visual cortex. Neuron 48(4):577-89. PubMed: 16301175 MGI: J:107593
Cang J; Niell CM; Liu X; Pfeiffenberger C; Feldheim DA; Stryker MP. 2008. Selective disruption of one Cartesian axis of cortical maps and receptive fields by deficiency in ephrin-As and structured activity. Neuron 57(4):511-23. PubMed: 18304481 MGI: J:132879
Cang J; Wang L; Stryker MP; Feldheim DA. 2008. Roles of ephrin-as and structured activity in the development of functional maps in the superior colliculus. J Neurosci 28(43):11015-23. PubMed: 18945909 MGI: J:143794
Chadaram SR; Laskowski MB; Madison RD. 2007. Topographic specificity within membranes of a single muscle detected in vitro. J Neurosci 27(51):13938-48. PubMed: 18094231 MGI: J:141637
Cooper MA; Son AI; Komlos D; Sun Y; Kleiman NJ; Zhou R. 2008. Loss of ephrin-A5 function disrupts lens fiber cell packing and leads to cataract. Proc Natl Acad Sci U S A 105(43):16620-5. PubMed: 18948590 MGI: J:144621
Cutforth T; Moring L; Mendelsohn M; Nemes A; Shah NM; Kim MM; Frisen J; Axel R. 2003. Axonal ephrin-As and odorant receptors: coordinate determination of the olfactory sensory map. Cell 114(3):311-22. PubMed: 12914696 MGI: J:101720
Defourny J; Poirrier AL; Lallemend F; Mateo Sanchez S; Neef J; Vanderhaeghen P; Soriano E; Peuckert C; Kullander K; Fritzsch B; Nguyen L; Moonen G; Moser T; Malgrange B. 2013. Ephrin-A5/EphA4 signalling controls specific afferent targeting to cochlear hair cells. Nat Commun 4:1438. PubMed: 23385583 MGI: J:226226
Depaepe V; Suarez-Gonzalez N; Dufour A; Passante L; Gorski JA; Jones KR; Ledent C; Vanderhaeghen P. 2005. Ephrin signalling controls brain size by regulating apoptosis of neural progenitors. Nature 435(7046):1244-50. PubMed: 15902206 MGI: J:99355
Deschamps C; Faideau M; Jaber M; Gaillard A; Prestoz L. 2009. Expression of ephrinA5 during development and potential involvement in the guidance of the mesostriatal pathway. Exp Neurol 219(2):466-80. PubMed: 19576892 MGI: J:154889
Dufour A; Seibt J; Passante L; Depaepe V; Ciossek T; Frisen J; Kullander K; Flanagan JG; Polleux F; Vanderhaeghen P. 2003. Area specificity and topography of thalamocortical projections are controlled by ephrin/Eph genes. Neuron 39(3):453-65. PubMed: 12895420 MGI: J:85411
Ellsworth CA; Lyckman AW; Feldheim DA; Flanagan JG; Sur M. 2005. Ephrin-A2 and -A5 influence patterning of normal and novel retinal projections to the thalamus: conserved mapping mechanisms in visual and auditory thalamic targets. J Comp Neurol 488(2):140-51. PubMed: 15924339 MGI: J:99706
Feng G; Laskowski MB; Feldheim DA; Wang H; Lewis R; Frisen J; Flanagan JG; Sanes JR. 2000. Roles for ephrins in positionally selective synaptogenesis between motor neurons and muscle fibers. Neuron 25(2):295-306. PubMed: 10719886 MGI: J:60774
Frisen J; Yates PA; McLaughlin T; Friedman GC; O'Leary DD; Barbacid M. 1998. Ephrin-A5 (AL-1/RAGS) is essential for proper retinal axon guidance and topographic mapping in the mammalian visual system. Neuron 20(2):235-43. PubMed: 9491985 MGI: J:45896
Hara Y; Nomura T; Yoshizaki K; Frisen J; Osumi N. 2010. Impaired hippocampal neurogenesis and vascular formation in ephrin-A5-deficient mice. Stem Cells 28(5):974-83. PubMed: 20474079 MGI: J:168862
Haustead DJ; Lukehurst SS; Clutton GT; Bartlett CA; Dunlop SA; Arrese CA; Sherrard RM; Rodger J. 2008. Functional topography and integration of the contralateral and ipsilateral retinocollicular projections of ephrin-A-/- mice. J Neurosci 28(29):7376-86. PubMed: 18632942 MGI: J:139255
Hjorth JJ; Sterratt DC; Cutts CS; Willshaw DJ; Eglen SJ. 2015. Quantitative assessment of computational models for retinotopic map formation. Dev Neurobiol 75(6):641-66. PubMed: 25367067 MGI: J:242263
Holmberg J; Clarke DL; Frisen J. 2000. Regulation of repulsion versus adhesion by different splice forms of an Eph receptor. Nature 408(6809):203-6. PubMed: 11089974 MGI: J:77779
Lehigh KM; Leonard CE; Baranoski J; Donoghue MJ. 2013. Parcellation of the thalamus into distinct nuclei reflects EphA expression and function. Gene Expr Patterns 13(8):454-63. PubMed: 24036135 MGI: J:202990
Lyckman AW; Jhaveri S; Feldheim DA; Vanderhaeghen P; Flanagan JG; Sur M. 2001. Enhanced plasticity of retinothalamic projections in an ephrin-A2/A5 double mutant. J Neurosci 21(19):7684-90. PubMed: 11567058 MGI: J:71649
Noh H; Lee H; Park E; Park S. 2016. Proper closure of the optic fissure requires ephrin A5-EphB2-JNK signaling. Development 143(3):461-72. PubMed: 26839344 MGI: J:240082
Otal R; Burgaya F; Frisen J; Soriano E; Martinez A. 2006. Ephrin-A5 modulates the topographic mapping and connectivity of commissural axons in murine hippocampus. Neuroscience 141(1):109-21. PubMed: 16690216 MGI: J:110251
Passante L; Gaspard N; Degraeve M; Frisen J; Kullander K; De Maertelaer V; Vanderhaeghen P. 2008. Temporal regulation of ephrin/Eph signalling is required for the spatial patterning of the mammalian striatum. Development 135(19):3281-90. PubMed: 18755772 MGI: J:138785
Pfeiffenberger C; Cutforth T; Woods G; Yamada J; Renteria RC; Copenhagen DR; Flanagan JG; Feldheim DA. 2005. Ephrin-As and neural activity are required for eye-specific patterning during retinogeniculate mapping. Nat Neurosci 8(8):1022-7. PubMed: 16025107 MGI: J:101436
Pfeiffenberger C; Yamada J; Feldheim DA. 2006. Ephrin-As and patterned retinal activity act together in the development of topographic maps in the primary visual system. J Neurosci 26(50):12873-84. PubMed: 17167078 MGI: J:116767
Prakash N; Vanderhaeghen P; Cohen-Cory S; Frisen J; Flanagan JG; Frostig RD. 2000. Malformation of the functional organization of somatosensory cortex in adult ephrin-A5 knock-out mice revealed by in vivo functional imaging. J Neurosci 20(15):5841-7. PubMed: 10908626 MGI: J:63611
Sheleg M; Yochum CL; Richardson JR; Wagner GC; Zhou R. 2015. Ephrin-A5 regulates inter-male aggression in mice. Behav Brain Res 286:300-7. PubMed: 25746458 MGI: J:223950
Sheleg M; Yochum CL; Wagner GC; Zhou R; Richardson JR. 2013. Ephrin-A5 deficiency alters sensorimotor and monoaminergic development. Behav Brain Res 236(1):139-47. PubMed: 22954718 MGI: J:193948
Son AI; Cooper MA; Sheleg M; Sun Y; Kleiman NJ; Zhou R. 2013. Further analysis of the lens of ephrin-A5-/- mice: development of postnatal defects. Mol Vis 19:254-66. PubMed: 23401654 MGI: J:195994
Son AI; Sheleg M; Cooper MA; Sun Y; Kleiman NJ; Zhou R. 2014. Formation of persistent hyperplastic primary vitreous in ephrin-A5-/- mice. Invest Ophthalmol Vis Sci 55(3):1594-606. PubMed: 24550361 MGI: J:229511
Suetterlin P; Drescher U. 2014. Target-independent ephrina/EphA-mediated axon-axon repulsion as a novel element in retinocollicular mapping. Neuron 84(4):740-52. PubMed: 25451192 MGI: J:223076
Torii M; Hashimoto-Torii K; Levitt P; Rakic P. 2009. Integration of neuronal clones in the radial cortical columns by EphA and ephrin-A signalling. Nature 461(7263):524-8. PubMed: 19759535 MGI: J:153504
Triplett JW; Phan A; Yamada J; Feldheim DA. 2012. Alignment of multimodal sensory input in the superior colliculus through a gradient-matching mechanism. J Neurosci 32(15):5264-71. PubMed: 22496572 MGI: J:184449
Wang L; Klein R; Zheng B; Marquardt T. 2011. Anatomical Coupling of Sensory and Motor Nerve Trajectory via Axon Tracking. Neuron 71(2):263-77. PubMed: 21791286 MGI: J:174684
Wilks TA; Rodger J; Harvey AR. 2010. A role for ephrin-As in maintaining topographic organization in register across interconnected central visual pathways. Eur J Neurosci 31(4):613-22. PubMed: 20384808 MGI: J:159859
Yabuta NH; Butler AK; Callaway EM. 2000. Laminar specificity of local circuits in barrel cortex of ephrin-A5 knockout mice. J Neurosci 20(15):RC88. PubMed: 10899175 MGI: J:70429
Yumoto N; Wakatsuki S; Kurisaki T; Hara Y; Osumi N; Frisen J; Sehara-Fujisawa A. 2008. Meltrin beta/ADAM19 interacting with EphA4 in developing neural cells participates in formation of the neuromuscular junction. PLoS One 3(10):e3322. PubMed: 18830404 MGI: J:144429

Service	Genotype	Price
	Heterozygous for Efna2<tm1Jgf>, Heterozygous for Efna5<tm1Ddmo>

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Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Efna2tm1Jgf

Allele Symbol: Efna2tm1Jgf

Efna5tm1Ddmo

Allele Symbol: Efna5tm1Ddmo

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Efna2tm1Jgf/Efna2+ Efna5tm1Ddmo/Efna5+involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ

nervous system phenotype

Genotype: Efna2tm1Jgf/Efna2tm1Jgf Efna5tm1Ddmo/Efna5tm1Ddmoinvolves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ

nervous system phenotype

References

Additional - Efna2tm1Jgf related

Additional - Efna5tm1Ddmo related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Progeny testing is not required

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Efna2^tm1Jgf

Allele Symbol: Efna2^tm1Jgf

Efna5^tm1Ddmo

Allele Symbol: Efna5^tm1Ddmo

Genotype: Efna2^tm1Jgf/Efna2⁺ Efna5^tm1Ddmo/Efna5⁺
involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ

Genotype: Efna2^tm1Jgf/Efna2^tm1Jgf Efna5^tm1Ddmo/Efna5^tm1Ddmo
involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ

Additional - Efna2^tm1Jgf related

Additional - Efna5^tm1Ddmo related