Overview

These ephrin A2/ephrin A5 double mutant mice may be useful in studies of retinocollicular mapping, axon topography, neuron projection, and modality-specific compartmentalization of visual, auditory, and somatosensory thalamic relay pathways.

Donating Investigator

David Feldhiem, University of California at Santa Cruz

Genetic overview

Genetic Background	Generation

Efna5^tm1Ddmo

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Efna5	ephrin A5

Efna2^tm1Jgf

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Efna2	ephrin A2

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Research Applications

Research Tools
Developmental Biology Research
Neurobiology Research
Sensorineural Research

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Mice homozygous for both targeted mutations are viable, fertile, and normal in size. While the donating investigator reports that 10-20% of females homozygous at both loci neglect their litters, no such neglect is reported in the colonies at The Jackson Laboratory (Aug 2009). Double homozygous mice have no endogenous protein expression in inferior colliculus (IC) or superior colliculus (SC), and thus lack the concentration gradient created by the endogenous proteins across the midbrain in wildtype mice. Temporal and nasal retinal axon termination is severely altered: multiple ectopic aborizations in the SC indicate abnormalities in both anteroposterior and dorsoventral topography. Following surgical ablation of portions of the midbrain (including IC and SC), cross-modal innervation by retinal neurons is greater in double homozygous mutants compared to wildtype. Mice heterozygous at both loci are reported to have greater reproductive performance compared to double homozygous mice. Further, double heterozygotes have temporal (but not nasal) retinal axon aborization in the SC with diminished frequency and severity. These ephrin A2/ephrin A5 double mutant mice may be useful in studies of retinocollicular mapping, axon topography, neuron projection, and modality-specific compartmentalization of visual, auditory, and somatosensory thalamic relay pathways.

Development

These mice harbor two targeted mutations; ephrin A2 and ephrin A5. For the ephrin A2 targeted mutation, a targeting vector was designed to insert a neomycin cassette in exon 2 after amino acid 66 (and just upstream of a cysteine repeat motif conserved throughout the ephrin family) of the Efna2 gene. The construct was electroporated into 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. Chimeric mice were bred with 129/SvEv mice. Mutant offspring were crossed to create homozygous mutant mice (Efna2^-/-). The ephrin A5 targeted mutation was independently created by replacing the portion of Efna5 exon 2 encoding amino acids 42-129 with a PGK-neo cassette. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 ES cells. Chimeric mice were bred with C57BL/6 mice. Mutant offspring were crossed to Swiss Webster mice and then made homozygous for this mutation (Efna5^-/-). Double mutant mice were obtained by crossing Efna2^-/- in a 129/SvEv background with a Efna5^-/- in a mixed background (Swiss-Webster, C57BL/6, 129). Upon arrival at The Jackson Laboratory, double mutant mice were bred with C57BL/6J for at least one generation to establish the colony.

Control Suggestions

None Available

Additional Information

Considerations for Choosing Controls

Selected References

Feldheim DA; Kim YI; Bergemann AD; Frisen J; Barbacid M; Flanagan JG. 2000. Genetic analysis of ephrin-A2 and ephrin-A5 shows their requirement in multiple aspects of retinocollicular mapping [see comments] Neuron 25(3):563-74PubMed: 10774725 MGI: J:61499

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Genetics

Efna5^tm1Ddmo

Allele Symbol: Efna5^tm1Ddmo

Allele Name	targeted mutation 1, Dennis D M O'Leary
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	A5^-; eA5KO; ephrin-A5^-
Gene Symbol and Name	Efna5, ephrin A5
Gene Synonym(s)
Strain of Origin	(129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Chromosome	17
Molecular Note	A neomycin selection cassette replaced sequence encoding amino acids 42 through 129 as well as a 5' splice acceptor. RT-PCR analysis showed an absence of transcript in homozygous mutant mice.

Efna2^tm1Jgf

Allele Symbol: Efna2^tm1Jgf

Allele Name	targeted mutation 1, John G Flanagan
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	A2^-; ephrin-A2^-
Gene Symbol and Name	Efna2, ephrin A2
Gene Synonym(s)
Strain of Origin	129S6/SvEvTac
Chromosome	10
Molecular Note	A neomycin selection cassette, containing stop codons in all three reading frames, was inserted after the sequence encoding amino acid 66. The insertion was upstream of a conserved cysteine residue motif. RT-PCR analysis of midbrain RNA showed an absence of transcript in homozygous mutant embryos.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Sensorineural Research
- Neurobiology Research
Developmental Biology Research
- Neurodevelopmental Defects
- Internal/Organ Defects
  - brain
Neurobiology Research
- Neurodevelopmental Defects
Sensorineural Research
- Eye Defects

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Efna2^tm1Jgf/Efna2^tm1Jgf Efna5^tm1Ddmo/Efna5^tm1Ddmo
involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ

nervous system phenotype

abnormal sensory neuron innervation pattern
- ectopic arborization of both retinal temporal axons and nasal axons are more sever than in singly homozygous mice
- normal arborization sometimes lost
- usually multiple ectopic arborizations are seen

Genotype: Efna2^tm1Jgf/Efna2⁺ Efna5^tm1Ddmo/Efna5⁺
involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ

nervous system phenotype

abnormal sensory neuron innervation pattern
- single ectopic arborizations of temporal axons from the retina but normal nasal arborizations

References

Feldheim DA; Kim YI; Bergemann AD; Frisen J; Barbacid M; Flanagan JG. 2000. Genetic analysis of ephrin-A2 and ephrin-A5 shows their requirement in multiple aspects of retinocollicular mapping [see comments] Neuron 25(3):563-74PubMed: 10774725 MGI: J:61499

Additional - Efna2^tm1Jgf related

Additional - Efna5^tm1Ddmo related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Efna2
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, mice that are homozygous for the Efna2^tm1Jgf and heterozygous for the Efna5^tm1Ddmo mutations are bred to mice that are heterozygous for the Efna2^tm1Jgf and homozygous for the Efna5^tm1Ddmo mutation. The donating investigator reports that 10-20% of females homozygous at both loci neglect their litters.
- Additional Breeding and Husbandry Support
Citation
When using the STOCK Efna2^tm1Jgf Efna5^tm1Ddmo/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #005992 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous for Efna2<tm1Jgf>, Heterozygous for Efna5<tm1Ddmo>

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

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Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Efna5tm1Ddmo

Allele Symbol: Efna5tm1Ddmo

Efna2tm1Jgf

Allele Symbol: Efna2tm1Jgf

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Efna2tm1Jgf/Efna2tm1Jgf Efna5tm1Ddmo/Efna5tm1Ddmoinvolves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ

nervous system phenotype

Genotype: Efna2tm1Jgf/Efna2+ Efna5tm1Ddmo/Efna5+involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ

nervous system phenotype

References

Additional - Efna2tm1Jgf related

Additional - Efna5tm1Ddmo related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Efna5^tm1Ddmo

Allele Symbol: Efna5^tm1Ddmo

Efna2^tm1Jgf

Allele Symbol: Efna2^tm1Jgf

Genotype: Efna2^tm1Jgf/Efna2^tm1Jgf Efna5^tm1Ddmo/Efna5^tm1Ddmo
involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ

Genotype: Efna2^tm1Jgf/Efna2⁺ Efna5^tm1Ddmo/Efna5⁺
involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ

Additional - Efna2^tm1Jgf related

Additional - Efna5^tm1Ddmo related