These Icam1 knock-out mice exhibit diabetes resistance.
Stephan Martin, Dusseldorf at the Heinrich-Heine-Univers
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Icam1 | intercellular adhesion molecule 1 |
No membrane bound ICAM-1 is detectable in NOD.129S4(B6)-Icam1tm1Jcgr/SmtnJ homozygous mice. NOD mice homozygous for the Icam1tm1Jcgr mutation are diabetes resistant. Histological exam shows no or mild peri-insular infiltration at 330-410 days of age, while 64% of their wild-type cohorts and 44% of the heterozygote animals become diabetic by 310 days of age. In contrast to wild-type, 110-day-old ICAM-1 deficient females do not develop severe insulitis 10 days post cyclophosphamide treatment. Protection is associated with suppression of destructive insulitis as well as a deviation to a T-helper 2 cytokine mRNA expression pattern (Martin et al. 2001).
Both the C57BL/6 contaminated strain and the non contaminated strain, Stock No. 006351 - NOD.129S4(B6)-Icam1tm1Jcgr/J are completely diabetes resistant at 30 weeks of age when evaluated in a comparative diabetes incidence study. Fine mapping data may be viewed at GQC of Icam1 congenic stocks
NOD.129S4(B6)-Icam1tm1Jcgr/SmtnJ is useful for studying the ICAM-1/LAF-1 interaction and its role in diabetes.
Intercellular adhesion molecule 1 (Icam1), located on Chr9, 7cM, contains 7 exons and is one of the immunoglobulin superfamily that binds to the integrins LFA-1 and Mac-1 on leukocytes. A neomycin resistance cassette was inserted into exon 4 (one of the 5 Ig-like extracellular domains) of the Icam1 gene. J1 ES cell clone 74 was injected into B6 blastocysts. This Icam1 mutation has been backcrossed to NOD for at least 6 generations (Martin et al, 2001). The Type 1 Diabetes Resource received NOD.129S4(B6)-Icam1tm1Jcgr/SmtnJ in 2005.
Additional genetic quality control performed at The Jackson Laboratory indicates C57BL/6 genomic contamination on Chromosomes 6 and 7. Markers D6Mit178, D6Mit52 and D6Mit135 on Chromosome 6 were segregating for both NOD and C57BL/6. Through selective breeding we have been able to make the Chromosome 6 markers of NOD origin only. Markers D7Mit139, D7Mit96 and D7Mit328 on Chromosome 7 were found to be of C57BL/6 origin. This stock is available with the Chromosome 7 genomic contamination and may affect the diabetes incidence.
Allele Name | targeted mutation 1, Jose Carlos Gutierrez Ramos |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | ICAMtm1-jcgr; ICAM-1-; ICAM-1-deficient |
Gene Symbol and Name | Icam1, intercellular adhesion molecule 1 |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae |
Chromosome | 9 |
Molecular Note | A neomycin resistance cassette was inserted into exon 4 of the gene. While King, et. al. (J:25530) reported the potential for the production of functional proteins arising from alternative transcripts, corresponding signals were undetected by immunohistochemical analysis of homozygous mutant mice. |
Mutations Made By | Dr. Jose-Carlos Gutierrez-Ramos, Millenium Pharmaceuticals |
When using the NOD.ICAM1null mouse strain in a publication, please cite the originating article(s) and include JAX stock #005983 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Icam1<tm1Jcgr> |
Frozen Mouse Embryo | NOD.129S4(B6)-Icam1<tm1Jcgr>/SmtnJ | $2595.00 |
Frozen Mouse Embryo | NOD.129S4(B6)-Icam1<tm1Jcgr>/SmtnJ | $2595.00 |
Frozen Mouse Embryo | NOD.129S4(B6)-Icam1<tm1Jcgr>/SmtnJ | $3373.50 |
Frozen Mouse Embryo | NOD.129S4(B6)-Icam1<tm1Jcgr>/SmtnJ | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.