Homozygous Dgat2tm1Far mice exhibit perinatal lethality and newborns are hypoglycemic and lipopenic, exhibiting reduced plasma triglyceride, free fatty acid and glucose levels and have almost no white fat tissue.
Robert V Farese, Jr., Harvard University School of Public Health
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Dgat2 | diacylglycerol O-acyltransferase 2 |
Mice that are homozygous for the targeted mutation have a perinatal lethal phenotype, most fail to survive past 6 hours after birth due to dehydration, skin barrier abnormalities, hypothermia and energy deficiencies. Reduced levels of gene product (mRNA) are detected by RT-PCR analysis of brown adipose tissue and livers of neonate homozygous mutants. Growth retardation is observed in homozygous embryos after embryonic day 12.5. Newborn homozygotes are hypoglycemic and lipopenic, exhibiting reduced plasma triglyceride, free fatty acid and glucose levels and have almost no white fat tissue. Within a few hours of birth, the skin of homozygotes becomes dry and cracked. Homozygous mutants rapidly lose weight due to dehydration caused by impaired permeability barrier function. Histological analysis of skin tissue from homozygotes reveals abnormally thin epidermis and compact orthohyperkeratosis of the stratum corneum. Homozygotes surviving between 8 and 24 hours develop tail necrosis. Heterozygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of embryonic development, essential fatty acid and triglyceride metabolism.
A targeting vector containing a floxed neo cassette and a herpes simplex virus thymidine kinase gene was used to disrupt exons 3 and 4. The construct was electroporated into 129S4/SvJae embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to the same for 8 generations.
Allele Name | targeted mutation 1, Bob Farese |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Dgat2-; Dgat2tm1Rvf |
Gene Symbol and Name | Dgat2, diacylglycerol O-acyltransferase 2 |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae |
Chromosome | 7 |
Molecular Note | The replacement of highly conserved exons 3 and 4 with a floxed neo cassette via homologous recombination introduces a premature stop codon. The encoded protein would be severely truncated (112 amino acids compared to 387), including 29 new amino acids. Homozygous mutant liver showed that diacylglycerol acyltransferase activity was reduced by 53%. |
Mutations Made By | Scot Stone, Gladstone Institutes |
When maintaining a live colony, these mice are bred as heterozygotes due to homozygous perinatal lethal phenotype.
When using the DGAT2 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #005951 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Dgat2<tm1Rvf> |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.