These Ucp3 knock-out mice exhibit aberrations in mitochondrial function. They are suitable for use in applications related to studies of energy metabolism.
Bradford B. Lowell, Beth Israel Deaconess Med Cntr (Harvard)
Homozygous mice are viable and fertile and do not express full length mRNA in skeletal muscle and brown adipose tissue. In skeletal muscle mitochondria, endogenous protein was undetectable. Skeletal muscle mitochondria from homozygous mice show decreased state 4 respiration. Further, skeletal muscle, but not liver, mitochondria produce more superoxide anions and reactive oxygen species both in vitro (lucigenin-derived chemiluminescence) and in vivo (aconitase activity). Skeletal muscle ATP synthesis under fasting conditions is approximately 4-fold greater in mutant mice. This mouse may be useful in studies of energy metabolism, thermogenesis, aerobic respiration (including mitochondrial respiration coupling), and age-related progressive deterioration of fatty acid homeostasis.
A targeting vector was created by replacing exons 2-3 of the endogenous gene with a PGK-Neo expression cassette. This construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and chimeric mice were bred to C57BL/6. The resulting heterozygotes were intercrossed for many generations before arrival at the The Jackson Laboratory. Upon arrival, these mice were bred to Stock No. 101043 (B6129SF1/J) for one generation and then maintained by heterozygous or homozygous matings.
|Allele Name||targeted mutation 1, Bradford B Lowell|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Ucp3, uncoupling protein 3 (mitochondrial, proton carrier)|
|Strain of Origin||129S4/SvJae|
|Molecular Note||A 700 bp genomic fragment containing part of exons 2 and 3 was replaced with a neomycin selection cassette. RNase protection analysis on RNA derived from skeletal muscle and brown adipose tissue of homozygous mice confirmed that no detectable transcript was expressed from this allele. Western blot analysis confirmed the absence of detectable protein in skeletal muscle mitochondria of homozygous mice.|
|Mutations Made By|| |
Bradford Lowell, Beth Israel Deaconess Med Cntr (Harvard)
When maintaining a live colony, these mice can be bred as homozygotes.
When using the UCP3 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #005937 in your Materials and Methods section.