Overview

Also Known As:BALB/c ByJ Cl.1 Thy1.1, BALB/c clone 1 Thy1.1

These Clone-1 TCR (also called Clone 1 Thy1.1 TCR or Cl.1 TCR) transgenic mice were designed to optimize conditions for tumor eradication by low avidity tumor-specific T cells and may also be useful in general studies of T cell avidity, tolerance, positive/negative selection, and activation.

Donating Investigator

Linda Sherman, The Scripps Research Institute

Genetic overview

Genetic Background	Generation

Tg(TcraCl1,TcrbCl1)1Shrm

Allele Type
Transgenic (Inserted expressed sequence)

Thy1^a

Allele Type	Gene Symbol	Gene Name
Not Applicable	Thy1	thymus cell antigen 1, theta

View Genetics

Research Applications

Immunology, Inflammation and Autoimmunity Research
Research Tools
Cancer Research

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Male mice that are hemizygous for the Clone-1 TCR (also called Clone 1 Thy1.1 TCR or Cl.1 TCR) transgene are viable, fertile, and normal in size. Females are very weak and have low fecundity. The donating investigator reports that all transgenic mice are prone to tumor development by 5-6 months of age. The transgene encodes a rearranged low avidity T cell receptor that recognizes an influenza virus hemagglutinin epitope (HA_518-526) restricted by MHC class I H-2K^d. The phenotype below is as defined by the B10.D2 background (see Stock No. 005895). Flow cytometric analysis shows appropriate skewing towards the CD8⁺ T cell compartment in thymocytes and peripheral lymphocytes. CD8⁺ T cells can be induced to exhibit both effector function and antitumor activity. This mouse is further modified with the Thy1^a allele, rather than the alternate allele present in C57BL/10, DBA/2, and BALB/c mice. Thus, cell populations derived from these transgenic mice can be distinguished from syngeneic host and other mice with the alternate allele via flow cytometry. These Clone-1 TCR (also called Clone 1 Thy1.1 TCR or Cl.1 TCR) transgenic mice were designed to optimize conditions for tumor eradication by low avidity tumor-specific T cells and may also be useful in general studies of T cell avidity, tolerance, positive/negative selection, and activation.

Of note, Clone-1 TCR mice are also available on a C57BL/10 congenic background (see Stock No. 005895).

In an attempt to offer alleles on well-characterized or multiplegenetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

A low avidity influenza hemagglutinin (HA)-specific CD8⁺ T cell clone (Clone 1) was expanded from the tolerant repertoire of a transgenic mouse expressing HA as a self-antigen on insulin-producing beta cells of the pancreatic islets (InsHA mouse). This T cell receptor sequence, specific for influenza virus HA_518-526 epitope restricted by MHC class I H-2K^d, was cloned into Tcra-V1,Tcra-J(J11) and Tcrb-V8.2,Tcrb-D,Tcrb-J (J2.4) expression vectors. The facility generating this mutant used "B10D2/nSnJ" mice derived from Stock No. 000463 as founder stock. The constructs were co-injected into fertilized B10D2/nSnJ eggs. Transgenic mice were bred to B10D2/nSnJ mice for 8 generations. Thereafter, transgenic mice were bred to B10D2/nSnJ mice homozygous for the Thy1^a allele (coding for the Thy1.1 leukocyte alloantigen) to generate transgenic mice homozygous for Thy1^a. After this, mutant mice were backcrossed to BALB/cByJ for 13 generations resulting in Clone 1 transgenic mice homozygous for the BALB/c-derived Thy1^b (coding for the Thy1.2 leukocyte alloantigen). Upon arrival at The Jackson Laboratory, transgenic Thy1^b/b males were bred with Stock No. 005443 (BALB/cByJ backcrossed mice homozygous for Thy1^a) or wildtype Thy1^a/a sibling females to generate Clone 1 transgenic Thy1^a/a mice.

Expression Data

Expressed Gene	Tcrb, T cell receptor beta chain, mouse, laboratory
Expressed Gene	Tcra, T cell receptor alpha chain, mouse, laboratory
Site of Expression
Site of Expression	The Thy1 locus determines a surface antigen present on cells of the thymus, a number of mouse leukemias, brain, and in lesser amounts on lymph node and spleen cells.

Control Suggestions

Noncarrier

Additional Information

Considerations for Choosing Controls

Selected References

Lyman MA; Nugent CT; Marquardt KL; Biggs JA; Pamer EG; Sherman LA. 2005. The fate of low affinity tumor-specific CD8+ T cells in tumor-bearing mice. J Immunol 174(5):2563-72PubMed: 15728462 MGI: J:97745

View All References

Genetics

Tg(TcraCl1,TcrbCl1)1Shrm

Allele Symbol: Tg(TcraCl1,TcrbCl1)1Shrm

Allele Name	transgene insertion 1, Linda Sherman
Allele Type	Transgenic (Inserted expressed sequence)
Allele Synonym(s)	Clone-1 TCR
Gene Symbol and Name	Tg(TcraCl1,TcrbCl1)1Shrm, transgene insertion 1, Linda Sherman
Gene Synonym(s)
Promoter	Tcrb, T cell receptor beta chain, mouse, laboratory
Promoter	Tcra, T cell receptor alpha chain, mouse, laboratory
Expressed Gene	Tcrb, T cell receptor beta chain, mouse, laboratory
Expressed Gene	Tcra, T cell receptor alpha chain, mouse, laboratory
Strain of Origin	B10.D2-Hc¹ H2^d H2-T18^c/nSnJ
Chromosome	UN
General Note	CD8⁺ T cells bearing this transgene, when transferred to mice that develop pancreatic tumors expressing influenza virus HA, can be induced to exhibit both effector function and antitumor activity only if HA-responsive CD4⁺ T cells are co-transferred and the mice are subsequently infected with influenza virus. : J:97745
Molecular Note	The transgene encodes a low avidity T cell receptor (TCR) that recognizes the influenza virus hemagglutinin (HA) epitope HA518-526 in the context of the H2-K^d MHC class I molecule. The rearranged Tcra and Tcrb genes, which have joined Valpha1/Jalpha11 and Vbeta8.2/D/Jbeta2.4, respectively, were amplified from cytotoxic T lymphocyte (CTL) clone 1. Clone 1 was derived from a transgenic B10.D2 mouse that expressed influenza virus HA as a self-antigen in pancreatic beta cells, which resulted in production of lower-avidity TCRs than those made by wild-type B10.D2 mice after infection with influenza virus strain A/PR/8/32.
Mutations Made By	Linda Sherman, The Scripps Research Institute

Thy1^a

Allele Symbol: Thy1^a

Allele Name	a variant
Allele Type	Not Applicable
Allele Synonym(s)	thetaAKR; theta-AKR; Thy1.1; Thy-1.1; Thy1a
Gene Symbol and Name	Thy1, thymus cell antigen 1, theta
Gene Synonym(s)
Site of Expression	The Thy1 locus determines a surface antigen present on cells of the thymus, a number of mouse leukemias, brain, and in lesser amounts on lymph node and spleen cells.
Strain of Origin	Not Applicable
Chromosome	9
General Note	The Thy1 locus determines a surface antigen present on cells of the thymus, a number of mouse leukemias, brain, and in lesser amounts on lymph node and spleen cells. The allele Thy1^a determines an antigenic specificity, Thy-1.1, found in the AKR and RF strains; the allele Thy1^b determines an antigenic specificity, Thy-1.2, found in the C3HeB/Fe and many other strains (J:5243, J:5012, J:4469). The Thy1 antigen is probably present on all T lymphocytes and absent from all B lymphocytes, and it thus serves as a valuable T-cell marker (J:5243). It is very widely used in experiments designed to determine the distribution and function of T-cells. Thy1 specifies a T-cell surface glycoprotein, T25, with a molecular weight of 25 kDa (J:5707). The protein appears to be anchored in the cell membrane by a lipid that is either phosphotidylinositol or closely related to it (J:12016). Thy1 may function in the cell membrane as a signal transduction molecule (J:8333). The Thy1 locus, or possibly a gene closely linked to it, controls quantitative expression of a protein that is the same size as Thy1 and is expressed on thymus and brain but not on lymph node and spleen cells (J:7900).
Molecular Note	The allele Thy1^a determines an antigenic specificity, Thy-1.1, found in the AKR and RF strains.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Thy1^a related

Immunology, Inflammation and Autoimmunity Research
- CD Antigens, Antigen Receptors, and Histocompatibility Markers
Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - T cell specific surface marker
- Genetics Research
  - Tissue/Cell Markers: T cell specific surface marker
  - Tissue/Cell Markers

Cancer Research
- Tumor Resistance
Immunology, Inflammation and Autoimmunity Research
- Rearranged Antigen-Specific T Cell Receptor Transgenes
Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - T Cell Receptor Transgenics
- Cancer Research
  - anti-tumor activity

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Tg(TcraCl1,TcrbCl1)1Shrm/0
B10.Cg-Thy1 H2 Tg(TcraCl1,TcrbCl1)1Shrm

behavior/neurological phenotype

weakness

hematopoietic system phenotype

abnormal T cell subpopulation ratio
- mice hemizygous for this transgene exhibit skewing of thymic and lymph node T cell populations toward the CD8⁺ compartment

immune system phenotype

abnormal T cell subpopulation ratio
- mice hemizygous for this transgene exhibit skewing of thymic and lymph node T cell populations toward the CD8⁺ compartment

neoplasm

increased tumor incidence
- some, but not all, hemizygous transgenic mice develop tumors at 5-6 months of age

reproductive system phenotype

abnormal fertility/fecundity
- pregnant hemizygous female mice usually die before giving birth

References

Lyman MA; Nugent CT; Marquardt KL; Biggs JA; Pamer EG; Sherman LA. 2005. The fate of low affinity tumor-specific CD8+ T cells in tumor-bearing mice. J Immunol 174(5):2563-72PubMed: 15728462 MGI: J:97745

Additional - Thy1^a related

Additional - Tg(TcraCl1,TcrbCl1)1Shrm related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Tg(TcraCl1,TcrbCl1)1Shrm
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, breed trangenic positive/Thy1a positive males with Stock No. 005443 (BALB/cByJ-Thy1^a/a) or wildtype sibling (Thy1^a positive) females. Transgenic females are very poor breeders.
- Additional Breeding and Husbandry Support
Citation
When using the CBy.Cg-Thy1^a Tg(TcraCl1,TcrbCl1)1Shrm/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #005922 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Hemizygous or Non carrier for Tg(TcraCl1,TcrbCl1)1Shrm

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

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Also Known As:BALB/c ByJ Cl.1 Thy1.1, BALB/c clone 1 Thy1.1

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Tg(TcraCl1,TcrbCl1)1Shrm

Allele Symbol: Tg(TcraCl1,TcrbCl1)1Shrm

Thy1a

Allele Symbol: Thy1a

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Thy1a related

Mammalian Phenotype Terms by Genotype

Genotype: Tg(TcraCl1,TcrbCl1)1Shrm/0B10.Cg-Thy1 H2 Tg(TcraCl1,TcrbCl1)1Shrm

hematopoietic system phenotype

immune system phenotype

neoplasm

reproductive system phenotype

References

Additional - Thy1a related

Additional - Tg(TcraCl1,TcrbCl1)1Shrm related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Thy1^a

Allele Symbol: Thy1^a

Genotype: Thy1^a related

Genotype: Tg(TcraCl1,TcrbCl1)1Shrm/0
B10.Cg-Thy1 H2 Tg(TcraCl1,TcrbCl1)1Shrm

Additional - Thy1^a related