B6.Cg-Il10^tm1Cgn (D3Mit49-D3Mit348)/Lt

Stock number: 005912
Product name: BC-R2
Research areas: Cancer Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research

Description

These l10 knock-out mice develop spontaneous colitic lesions in the cecum and large intestine. These mice may be useful in studies relating to innate and adaptive immune response with the intestinal microbiota.

Detailed description

Homozygous mice are IL10-deficient and develop spontaneous colitic lesions in the cecum and large intestine as early as 6-8 weeks of age. The severity and age of onset of colitis varies with the donor type and location of the cytokine deficiency colitis susceptibility 1 (Cdcs1) interval. Generally, the C3H/HeJBir region encompassed by D3Mit348 through D3Mit254 (127.7-132.5 megabases) confers increased colitis susceptibility on the B6.129P2-Il10^tm1Cgn background (Beckwith J, et al., 2005). Mice on the B6.129P2-Il10^tm1Cgn background are prone to rectal prolapse particularly when carrying susceptibility alleles from Chromosome 3. Granulocyte populations in peripheral blood increase upon lesion development and provide a robust non-lethal assessment of colitis severity.

Development

A panel of reciprocal congenic stocks was generated by a marker assisted "speed congenic" approach. C3Bir.129P2(B6)-Il10^tm1Cgn/Lt (Stock No. 004326) mice were backcrossed to B6.129P2-Il10^tm1Cgn/JLt for 10 (N10) generations. Progeny were selected for a C3H/HeJBir donor interval within Cdcs1. This strain (Stock No. 005912) is called "BC-R2" in the primary reference (Beckwith 2005 Gastroenterology 129:1473), and contains the C3H/HeJBir donor interval defined by D3Mit49-D3Mit348. Of note, the BC-R2 mice described in Beckwith 2005 were backcrossed to B6.129P2-Il10^tm1Cgn/JLt between 4-6 generations (N4-N6) generations.

Allele

Symbol: Il10^tm1Cgn
Name: targeted mutation 1, University of Cologne
MGI accession ID: MGI:1857199
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Il10
Marker name: interleukin 10
Chromosome: 1
Strain of origin: 129P2/OlaHsd
Molecular note: A 500 bp genomic fragment containing codons 5-55 was replaced with a linker containing a termination codon followed by a neomycin cassette. A termination codon was also introduced into exon 3. No IL10 activity was detectable by ELISA assays in supernatants of in vitro cultures of Con A-stimulated splenic T cells derived from homozygous mice following infection with the nematode N. brasiliensis.

Allele

Symbol: Cdcs1^C3H/HeJBir
Name: C3H/HeJBir
MGI accession ID: MGI:2159154
Generation method: QTL
Marker symbol: Cdcs1
Marker name: cytokine deficiency colitis susceptibility 1
Chromosome: 3
Strain of origin: C3H/HeJBir
Molecular note: This allele confers colitis susceptibility compared to C57BL/6J.
General note: Cdcs1 exhibits additive inheritance.

Allele

MGI accession ID: MGI:105725
Marker symbol: D3Mit348
Marker name: DNA Segment, Chr 3 Massachusetts Institute of Technology 348
Chromosome: 3

Allele

MGI accession ID: MGI:92518
Marker symbol: D3Mit49
Marker name: DNA segment, Chr 3, Massachusetts Institute of Technology 49
Chromosome: 3