These l10 knock-out mice develop spontaneous colitic lesions in the cecum and large intestine. These mice may be useful in studies relating to innate and adaptive immune response with the intestinal microbiota.
Read More +Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Il10 | interleukin 10 |
Allele Type | Gene Symbol | Gene Name |
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QTL | Cdcs1 | cytokine deficiency colitis susceptibility 1 |
Marker Symbol | Marker Name | |
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D3Mit348 | DNA Segment, Chr 3 Massachusetts Institute of Technology 348 | |
D3Mit49 | DNA segment, Chr 3, Massachusetts Institute of Technology 49 |
Homozygous mice are IL10-deficient and develop spontaneous colitic lesions in the cecum and large intestine as early as 6-8 weeks of age. The severity and age of onset of colitis varies with the donor type and location of the cytokine deficiency colitis susceptibility 1 (Cdcs1) interval. Generally, the C3H/HeJBir region encompassed by D3Mit348 through D3Mit254 (127.7-132.5 megabases) confers increased colitis susceptibility on the B6.129P2-Il10tm1Cgn background (Beckwith J, et al., 2005). Mice on the B6.129P2-Il10tm1Cgn background are prone to rectal prolapse particularly when carrying susceptibility alleles from Chromosome 3. Granulocyte populations in peripheral blood increase upon lesion development and provide a robust non-lethal assessment of colitis severity.
A panel of reciprocal congenic stocks was generated by a marker assisted "speed congenic" approach. C3Bir.129P2(B6)-Il10tm1Cgn/Lt (Stock No. 004326) mice were backcrossed to B6.129P2-Il10tm1Cgn/JLt for 10 (N10) generations. Progeny were selected for a C3H/HeJBir donor interval within Cdcs1. This strain (Stock No. 005912) is called "BC-R2" in the primary reference (Beckwith 2005 Gastroenterology 129:1473), and contains the C3H/HeJBir donor interval defined by D3Mit49-D3Mit348. Of note, the BC-R2 mice described in Beckwith 2005 were backcrossed to B6.129P2-Il10tm1Cgn/JLt between 4-6 generations (N4-N6) generations.
Allele Name | targeted mutation 1, University of Cologne |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | IL-10-; Il10-; IL-10 KO; IL-10KO; Il10tmCgn; IL-10KO |
Gene Symbol and Name | Il10, interleukin 10 |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 1 |
Molecular Note | A 500 bp genomic fragment containing codons 5-55 was replaced with a linker containing a termination codon followed by a neomycin cassette. A termination codon was also introduced into exon 3. No IL10 activity was detectable by ELISA assays in supernatants of in vitro cultures of Con A-stimulated splenic T cells derived from homozygous mice following infection with the nematode N. brasiliensis. |
Mutations Made By | Dr. Ralf Kuhn, Max-Delbrück Center for Molecular Medicine |
Allele Name | C3H/HeJBir |
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Allele Type | QTL |
Allele Synonym(s) | Cdcs1C3Bir |
Gene Symbol and Name | Cdcs1, cytokine deficiency colitis susceptibility 1 |
Gene Synonym(s) | |
Strain of Origin | C3H/HeJBir |
Chromosome | 3 |
General Note | Cdcs1 exhibits additive inheritance. |
Molecular Note | This allele confers colitis susceptibility compared to C57BL/6J. |
Marker Synonym(s) | |
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Chromosome(s) | 3 |
Marker Synonym(s) | |
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Chromosome(s) | 3 |
When using the BC-R2 mouse strain in a publication, please cite the originating article(s) and include JAX stock #005912 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or Wild-type for Il10<tm1Cgn> (D3Mit49-D3Mit348) |
Frozen Mouse Embryo | B6.Cg-Il10<tm1Cgn> (D3Mit49-D3Mit348)/Lt Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Il10<tm1Cgn> (D3Mit49-D3Mit348)/Lt Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Il10<tm1Cgn> (D3Mit49-D3Mit348)/Lt Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Il10<tm1Cgn> (D3Mit49-D3Mit348)/Lt Frozen Embryo | $3373.50 |
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