Overview

Also Known As: tremor and reduced lifespan

Mice that are homozygous for this spontaneous mutation are characterized by tremors and small body size.

Genetic overview

Genetic Background	Generation

Hcn2^trls

Allele Type	Gene Symbol	Gene Name
Spontaneous	Hcn2	hyperpolarization-activated, cyclic nucleotide-gated K+ 2

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Research Applications

Neurobiology Research
Reproductive Biology Research
Developmental Biology Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Homozygotes can usually be identified at 14 days of age by a tremor and smaller than normal body size. Most homozygotes die around 3 weeks of age, although some have lived longer.

Development

The mutation tremor and reduced life span (trls) arose spontaneously at The Jackson Laboratory in the strain BKS.Cg-m +/+ Lepr^db/J in 2000. Because homozygotes are not suitable breeders, this mutant subline was maintained by breeding hosts of homozygous ovarian transplant with donor sibling males. This was done for 10 generations before being maintained by ovarian transplant backcross-intercross to C57BLKS/J. In 2005 this strain reached generation F10N4 and the misty (m) and diabetic (Lepr^db) mutations have not been seen since the initial generation so it is expected that they have been bred out.

Control Suggestions

+/? untyped from the colony

Additional Information

Considerations for Choosing Controls

Selected References

Samples R; Ward-Bailey PF; Donahue LR; Johnson K; Wang J; Davisson M. 2005. Tremor and reduced lifespan (trls): a new neurological mutation on Chromosome 10 MGI Direct Data SubmissionMGI: J:103185

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Genetics

Hcn2^trls

Allele Symbol: Hcn2^trls

Allele Name	tremor and reduced lifespan
Allele Type	Spontaneous
Allele Synonym(s)	tremor and reduced lifespan; Hcn2^trls
Gene Symbol and Name	Hcn2, hyperpolarization-activated, cyclic nucleotide-gated K+ 2
Gene Synonym(s)	BCNG2; trls; HAC1; BCNG-2; HAC-1; tremor and reduced lifespan; trls
Strain of Origin	BKS.Cg-Dock7^m +/+ Lepr^db/J
Chromosome	10
Molecular Note	This spontaneous mutation has a G to A transition at Chromosome 10 position 79187525 (NCBI Build 37, mm9) that is predicted to cause an arginine to glutamine substitution at residue 315.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Hcn2^trls related

Neurobiology Research
- Tremor Defects
Reproductive Biology Research
- Developmental Defects Affecting Gonads
  - males only
- Fertility Defects
  - males only
Developmental Biology Research
- Postnatal Lethality
  - Homozygous

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Hcn2^trls/Hcn2^trls
Not Specified

behavior/neurological phenotype

tremors
- tremor in 14 day old pups is similar to that seen with other demyelinating mutations

cellular phenotype

azoospermia
- absent sperm at 3 weeks of age in 3 mutant male mice; one 9 week old mouse did not show this phenotype

growth/size/body region phenotype

decreased body size
- smaller body size is evident at 14 days of age

hearing/vestibular/ear phenotype

increased or absent threshold for auditory brainstem response
- variable penetrance; mild hearing impairment is shown by an increased threshold for high frequency stimuli, but the wave pattern is similar to controls unlike in demyelinating mutations

mammalian phenotype

nervous system phenotype
- Normal - peripheral and central nervous system myelination appears normal at 9 weeks of age

mortality/aging

lethality at weaning, incomplete penetrance
- most homozygous mice die at 3 weeks of age, although a few may survive longer

reproductive system phenotype

azoospermia
- absent sperm at 3 weeks of age in 3 mutant male mice; one 9 week old mouse did not show this phenotype

References

Samples R; Ward-Bailey PF; Donahue LR; Johnson K; Wang J; Davisson M. 2005. Tremor and reduced lifespan (trls): a new neurological mutation on Chromosome 10 MGI Direct Data SubmissionMGI: J:103185

Additional - Hcn2^trls related

Technical Support

Contact Technical Support

Genotyping Protocols
- Genotyping resources and troubleshooting
Citation
When using the tremor and reduced lifespan mouse strain in a publication, please cite the originating article(s) and include JAX stock #005898 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or wildtype for Hcn2<trls>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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General Terms and Conditions

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Licensing Information

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Also Known As: tremor and reduced lifespan

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Hcn2trls

Allele Symbol: Hcn2trls

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Hcn2trls related

Mammalian Phenotype Terms by Genotype

Genotype: Hcn2trls/Hcn2trlsNot Specified

behavior/neurological phenotype

cellular phenotype

growth/size/body region phenotype

hearing/vestibular/ear phenotype

mammalian phenotype

mortality/aging

reproductive system phenotype

References

Additional - Hcn2trls related

Genotyping Protocols

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Hcn2^trls

Allele Symbol: Hcn2^trls

Genotype: Hcn2^trls related

Genotype: Hcn2^trls/Hcn2^trls
Not Specified

Additional - Hcn2^trls related