Mice that are homozygous for this spontaneous mutation are characterized by tremors and small body size.
Read More +Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Spontaneous | Hcn2 | hyperpolarization-activated, cyclic nucleotide-gated K+ 2 |
Homozygotes can usually be identified at 14 days of age by a tremor and smaller than normal body size. Most homozygotes die around 3 weeks of age, although some have lived longer.
The mutation tremor and reduced life span (trls) arose spontaneously at The Jackson Laboratory in the strain BKS.Cg-m +/+ Leprdb/J in 2000. Because homozygotes are not suitable breeders, this mutant subline was maintained by breeding hosts of homozygous ovarian transplant with donor sibling males. This was done for 10 generations before being maintained by ovarian transplant backcross-intercross to C57BLKS/J. In 2005 this strain reached generation F10N4 and the misty (m) and diabetic (Leprdb) mutations have not been seen since the initial generation so it is expected that they have been bred out.
Allele Name | tremor and reduced lifespan |
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Allele Type | Spontaneous |
Allele Synonym(s) | tremor and reduced lifespan; Hcn2trls |
Gene Symbol and Name | Hcn2, hyperpolarization-activated, cyclic nucleotide-gated K+ 2 |
Gene Synonym(s) | BCNG2; trls; HAC1; BCNG-2; HAC-1; tremor and reduced lifespan; trls |
Strain of Origin | BKS.Cg-Dock7m +/+ Leprdb/J |
Chromosome | 10 |
Molecular Note | This spontaneous mutation has a G to A transition at Chromosome 10 position 79187525 (NCBI Build 37, mm9) that is predicted to cause an arginine to glutamine substitution at residue 315. |
When using the tremor and reduced lifespan mouse strain in a publication, please cite the originating article(s) and include JAX stock #005898 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service | Genotype | Price |
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Heterozygous or wildtype for Hcn2<trls> |
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
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