BKS(Cg)-Hcn2^trls/GrsrJ

Stock number: 005898
Product name: tremor and reduced lifespan
Research areas: Developmental Biology Research, Neurobiology Research, Reproductive Biology Research

Description

Mice that are homozygous for this spontaneous mutation are characterized by tremors and small body size.

Detailed description

Homozygotes can usually be identified at 14 days of age by a tremor and smaller than normal body size. Most homozygotes die around 3 weeks of age, although some have lived longer.

Development

The mutation tremor and reduced life span (trls) arose spontaneously at The Jackson Laboratory in the strain BKS.Cg-m +/+ Lepr^db/J in 2000. Because homozygotes are not suitable breeders, this mutant subline was maintained by breeding hosts of homozygous ovarian transplant with donor sibling males. This was done for 10 generations before being maintained by ovarian transplant backcross-intercross to C57BLKS/J. In 2005 this strain reached generation F10N4 and the misty (m) and diabetic (Lepr^db) mutations have not been seen since the initial generation so it is expected that they have been bred out.

Allele

Symbol: Hcn2^trls
Name: tremor and reduced lifespan
MGI accession ID: MGI:3582957
Generation method: Spontaneous
Marker symbol: Hcn2
Marker name: hyperpolarization-activated, cyclic nucleotide-gated K+ 2
Chromosome: 10
Strain of origin: BKS.Cg-Dock7^m +/+ Lepr^db/J
Molecular note: This spontaneous mutation has a G-to-A transition at chromosome 10 position 79,724,780 (GRCm38) that causes an arginine to glutamine substitution at residue 315 (p.R315Q).