These floxed mutant mice possess loxP sites flanking exon 4 of the Ppard gene. This strain may be useful for generating conditional mutations in applications related to embryo development and adipocyte physiology.
Yaacov Barak, University of Pittsburgh
These mice possess loxP sites on either side of exon 4 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice with a cre recombinase gene under the control of a promoter of interest, exon 4 of the targeted gene is deleted in the tissue of interest, leading to premature termination of the translation product upstream of the DNA binding domain. This strain may be useful in generating tissue-specific mutants of the floxed allele for use in studies including embryo development, adipocyte physiology, fat metabolism and storage, inflammation, and cancer.
A targeting vector was designed to place a single loxP site upstream of exon 4 of the endogenous gene and a loxP-flanked neomycin resistance-thymidine kinase gene cassette downstream of exon 4. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were then transiently transfected with a Cre-recombinase vector. Clones in which the selection cassette was selectively deleted, but the loxP-flanked exon 4 remained intact, were injected into C57BL/6J blastocysts. Chimeric mice were backcrossed to C57BL/6 mice for approximately 8 generations. The resulting heterozygotes were intercrossed to produce homozygotes.
|Allele Name||targeted mutation 1, Ronald M Evans|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Ppard, peroxisome proliferator activator receptor delta|
|Gene Synonym(s)||FAAR; NR1C2; NUC1; NUCI; NUCII; Nr1c2; PPAR-delta; PPARB; PPARdelta/beta; Peroxisome proliferator-activated receptor beta; Pparb; Pparb/d|
|Strain of Origin||Not Specified|
|Molecular Note||Exon 4, which encodes the amino terminal portion of the DNA binding domain was left flanked by single loxP sites after a downstream neo cassette was excised via the expression of cre recombinase in vitro.|
|Mutations Made By|| |
Ronald Evans, The Salk Inst for Biological Studies
When maintaining a live colony, these mice are bred as homozygotes.
When using the Pparδck mouse strain in a publication, please cite the originating article(s) and include JAX stock #005897 in your Materials and Methods section.
|Heterozygous or Wildtype for Ppard<tm1Rev>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
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