These floxed mutant mice possess loxP sites flanking exon 4 of the Ppard gene. This strain may be useful for generating conditional mutations in applications related to embryo development and adipocyte physiology.
Yaacov Barak, University of Pittsburgh
These mice possess loxP sites on either side of exon 4 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice with a cre recombinase gene under the control of a promoter of interest, exon 4 of the targeted gene is deleted in the tissue of interest, leading to premature termination of the translation product upstream of the DNA binding domain. This strain may be useful in generating tissue-specific mutants of the floxed allele for use in studies including embryo development, adipocyte physiology, fat metabolism and storage, inflammation, and cancer.
A targeting vector was designed to place a single loxP site upstream of exon 4 of the endogenous gene and a loxP-flanked neomycin resistance-thymidine kinase gene cassette downstream of exon 4. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were then transiently transfected with a Cre-recombinase vector. Clones in which the selection cassette was selectively deleted, but the loxP-flanked exon 4 remained intact, were injected into C57BL/6J blastocysts. Chimeric mice were backcrossed to C57BL/6 mice for approximately 8 generations. The resulting heterozygotes were intercrossed to produce homozygotes.
|Allele Name||targeted mutation 1, Ronald M Evans|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Ppard, peroxisome proliferator activator receptor delta|
|Strain of Origin||Not Specified|
|Molecular Note||Exon 4, which encodes the amino terminal portion of the DNA binding domain was left flanked by single loxP sites after a downstream neo cassette was excised via the expression of cre recombinase in vitro.|
|Mutations Made By|| |
Ronald Evans, The Salk Inst for Biological Studies
When maintaining a live colony, these mice are bred as homozygotes.
When using the Pparδck mouse strain in a publication, please cite the originating article(s) and include JAX stock #005897 in your Materials and Methods section.
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