This Il4 knockout strain is useful in studies of arthritis, autoimmunity, and T helper cell immune responses.
Linda K Myers, University of Tennessee, Memphis
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Il4 | interleukin 4 |
Mice homozygous for the targeted mutation are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. No endogenous gene product is detectable in splenocyte supernatants. Mutant mice crossed to the DBA/1LacJ background (I-Aq haplotype) are susceptible to collagen-induced arthritis. Deletion of the endogenous gene exacerbates both the incidence and severity of collagen-induced arthritis, which is accompanied with increased IgG2 and decreased IgG1 in sera. Following type II collagen (CII) tolerization of mutant mice, splenocytes cultured with CII have increased Th2 (IL-5, IL-9, IL-10, IL-13) and Th1 (IFNgamma, IL-6) cytokines. Mutant mice may be useful in other studies of collagen-induced arthritis, rheumatoid arthritis, mechanisms of tolerance, regulation of autoimmune disease, and T helper cell immune responses.
On the C57BL/6 background (see Stock No. 002253) from which this mouse was created, T and B cell development is normal but IgGl and IgE levels and the ability of homozygous mutant mice to produce Th2-derived cytokines are significantly reduced.
The targeted mutation was generated by disrupting exon 1 of the endogenous gene with a neomycin resistance gene. The construct was electroporated into 129P2/OlaHsd-derived E14-1 embryonic stem cells and the resulting chimeric animals were crossed to C57BL/6 mice for many generations. The mutant mice on DBA/1LacJ background were generated by crossing male B6.129P2-Il4tm1Cgn/J (see Stock No. 002253) with female DBA/1LacJ (see Stock No. 001140) mice for 12 generations before being intercrossed for homozygosity.
Allele Name | targeted mutation 1, University of Cologne |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | IL-4-; IL-4 KO; IL4-; IL4tm1cgn129; IL-4KO; IL4T; IL-4T- |
Gene Symbol and Name | Il4, interleukin 4 |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 11 |
Molecular Note | A translational stop codon and a neomycin resistance gene were inserted into the first exon of the gene. |
Mutations Made By | Dr. Ralf Kuhn, Max-Delbrück Center for Molecular Medicine |
When maintaining a live colony, these mice are bred as homozygotes.
When using the IL-4 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #005879 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Il4<tm1Cgn> |
Frozen Mouse Embryo | D1Lac.Cg-Il4<tm1Cgn>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | D1Lac.Cg-Il4<tm1Cgn>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | D1Lac.Cg-Il4<tm1Cgn>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | D1Lac.Cg-Il4<tm1Cgn>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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