This Il4 knockout strain is useful in studies of arthritis, autoimmunity, and T helper cell immune responses.
Linda K Myers, University of Tennessee, Memphis
Mice homozygous for the targeted mutation are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. No endogenous gene product is detectable in splenocyte supernatants. Mutant mice crossed to the DBA/1LacJ background (I-Aq haplotype) are susceptible to collagen-induced arthritis. Deletion of the endogenous gene exacerbates both the incidence and severity of collagen-induced arthritis, which is accompanied with increased IgG2 and decreased IgG1 in sera. Following type II collagen (CII) tolerization of mutant mice, splenocytes cultured with CII have increased Th2 (IL-5, IL-9, IL-10, IL-13) and Th1 (IFNgamma, IL-6) cytokines. Mutant mice may be useful in other studies of collagen-induced arthritis, rheumatoid arthritis, mechanisms of tolerance, regulation of autoimmune disease, and T helper cell immune responses.
On the C57BL/6 background (see Stock No. 002253) from which this mouse was created, T and B cell development is normal but IgGl and IgE levels and the ability of homozygous mutant mice to produce Th2-derived cytokines are significantly reduced.
The targeted mutation was generated by disrupting exon 1 of the endogenous gene with a neomycin resistance gene. The construct was electroporated into 129P2/OlaHsd-derived E14-1 embryonic stem cells and the resulting chimeric animals were crossed to C57BL/6 mice for many generations. The mutant mice on DBA/1LacJ background were generated by crossing male B6.129P2-Il4tm1Cgn/J (see Stock No. 002253) with female DBA/1LacJ (see Stock No. 001140) mice for 12 generations before being intercrossed for homozygosity.
|Allele Name||targeted mutation 1, University of Cologne|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||IL-4-; IL-4 KO; IL4-; IL4tm1cgn129; IL-4KO; IL4T; IL-4T-|
|Gene Symbol and Name||Il4, interleukin 4|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||A translational stop codon and a neomycin resistance gene were inserted into the first exon of the gene.|
|Mutations Made By|| |
Dr. Ralf Kuhn, Max-Delbrück Center for Molecular Medicine
When maintaining a live colony, these mice are bred as homozygotes.
When using the IL-4 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #005879 in your Materials and Methods section.