Overview

Also Known As: IDO KO

The lack of endogenous protein in homozygotes of these indoleamine 2,3-dioxygenase 1 knock-out mice affects immune and a number of other physiological responses. These mice may be useful in studies of pregnancy and reproductive immunology, as well as other immune response and physiological processes.

Donating Investigator

Andrew Mellor, Medical College of Georgia

Genetic overview

Genetic Background	Generation
	N10+N1pN2F4 (2019-03-04 00:00:00)

Ido1^tm1Alm

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Ido1	indoleamine 2,3-dioxygenase 1

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Research Applications

Immunology, Inflammation and Autoimmunity Research
Research Tools

View All Research Applications

Base Price

Starting at:

$236.78 Domestic price for female 4-week

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Details

Detailed Description

Homozygous mice are viable and fertile with normal immune system development and function. They exhibit no spontaneous autoimmune disorders. No gene product (mRNA or protein) from the targeted gene is detected in the epididymis. At embryonic day 10.5, endogenous protein is absent from all cells at the maternal-fetal interface when both parents are homozygous for the targeted gene. Allogeneic and syngeneic pregnancy outcomes are unaffected by this mutation. In contrast to wild-type, anti-proliferative treatments (CTLA4-Ig, IFNalpha, or CpG-ODN) do not suppress T cell expansion both in vivo and in vitro. In addition, homozygous dendritic cells isolated from lymph nodes draining (induced) tumor sites have no suppressor activity. These mice may be useful in studies of pregnancy and reproductive immunology (tryptophan degradation, T cell activation, clonal expansion) as well as autoimmune disease, tissue transplantation, fostering, acquired tolerance/T cell anergy, and immunosuppressive pathways.

Development

A targeting vector was designed to replace exons 3-5 (encode critical portions of the enzyme catalytic site) with the beta-galactosidase and neomycin resistance genes. A translational "stop" codon (TAG) that was reported to have been introduced into exon 2 is not detected in The Jackson Laboratory Repository colony. The construct was electroporated into 129/SvJ-derived embryonic stem (ES) cells and correctly targeted clones were injected into blastocysts. Male chimeric mice were mated with C57BL/6 females to produce heterozygotes. Mutant mice were backcrossed more than 10 generations to C57BL/6 before arriving at The Jackson Laboratory.

Control Suggestions

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Baban B; Chandler P; McCool D; Marshall B; Munn DH; Mellor AL. 2004. Indoleamine 2,3-dioxygenase expression is restricted to fetal trophoblast giant cells during murine gestation and is maternal genome specific. J Reprod Immunol 61(2):67-77PubMed: 15063630 MGI: J:93797

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Genetics

Ido1^tm1Alm

Allele Symbol: Ido1^tm1Alm

Allele Name	targeted mutation 1, Andrew L Mellor
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	targeted mutation 1, Andrew L Mellor; Ido1^tm1Alm
Gene Symbol and Name	Ido1, indoleamine 2,3-dioxygenase 1
Gene Synonym(s)	Ido; INDO; Indo; IDO; IDO-1; indoleamine-pyrrole 2,3 dioxygenase; indole 2,3-dioxygenase; indole 2,4-dioxygenase; Ido; Indo
Strain of Origin	129X1/SvJ
Chromosome	8
Molecular Note	A Betagal and neomycin cassette replaced exons 3-5, which encode critical portions of the enzyme catalytic site. Also, a translation stop codon was introduced into exon 2. RT-PCR of total epididymis RNA from mutants showed no transcript was present. Western blot of mutant epididymis confirmed a lack of protein.
Mutations Made By	Brendan Marshall, Medical College of Georgia

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
- Autoimmunity
- Immunodeficiency
  - T cell deficiency
Research Tools
- Reproductive Biology Research
- Immunology, Inflammation and Autoimmunity Research
  - T cell deficiency, xenograft/transplant host
- Cancer Research
  - B and T cell deficiency, xenograft/transplant host

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Ido1^tm1Alm/Ido1^tm1Alm
either: (involves: 129X1/SvJ * C57BL/6) or (involves: 129X1/SvJ * CBA)

mammalian phenotype

reproductive system phenotype
- Normal - no effect on reproductive success

Genotype: Ido1^tm1Alm/Ido1^tm1Alm
involves: 129X1/SvJ * BALB/c

cellular phenotype

abnormal autophagy
- lysosomal autophagy is seen in mutant but not wild-type caput epididymal tissues as indicated by an increase in multilamellar/multivesicular bodies

enlarged sperm head

hairpin sperm flagellum

pinhead sperm

teratozoospermia
- increase in the proportion of spermatozoa exhibiting abnormal morphology, including pinhead, giant head, hairpin and kite spermatozoa

immune system phenotype

abnormal cytokine level
- levels of some cytokines are higher in mutant caput epididymis

decreased leukocyte cell number
- decrease in leukocyte count in caudal epididymal fluid

epididymal inflammation
- whereas wild-type caput epididymis exhibits a high KYN/Trp ratio indicating that it is in an immunosuppressed state, mutants have a much lower KYN/Trp ratio, indicating that the caput epididymis is in an inflammatory state

mammalian phenotype

reproductive system phenotype
- Normal - males exhibit normal fertility and testis weight

reproductive system phenotype

abnormal caput epididymis morphology
- levels of some cytokines are higher in mutant caput epididymis
- lysosomal autophagy is seen in mutant but not wild-type caput epididymal tissues
- significantly greater amounts of protein in caput epididymis extracts than in controls

enlarged sperm head

epididymal inflammation
- whereas wild-type caput epididymis exhibits a high KYN/Trp ratio indicating that it is in an immunosuppressed state, mutants have a much lower KYN/Trp ratio, indicating that the caput epididymis is in an inflammatory state

hairpin sperm flagellum

increased sperm number
- caudal epididymal sperm counts are nearly twice as high in mutants as in wild-type males

pinhead sperm

teratozoospermia
- increase in the proportion of spermatozoa exhibiting abnormal morphology, including pinhead, giant head, hairpin and kite spermatozoa

hematopoietic system phenotype

decreased leukocyte cell number
- decrease in leukocyte count in caudal epididymal fluid

homeostasis/metabolism phenotype

abnormal autophagy
- lysosomal autophagy is seen in mutant but not wild-type caput epididymal tissues as indicated by an increase in multilamellar/multivesicular bodies

abnormal cytokine level
- levels of some cytokines are higher in mutant caput epididymis

abnormal protein level
- significantly greater amounts of protein in caput epididymis extracts than in controls due to decreased protein degradation

Genotype: Ido1^tm1Alm/Ido1^tm1Alm
involves: 129X1/SvJ

mammalian phenotype

homeostasis/metabolism phenotype
- Normal - tryptophan plasma levels are normal

Genotype: Ido1^tm1Alm/Ido1^tm1Alm
involves: 129X1/SvJ * C57BL/6J * FVB/N

immune system phenotype

decreased susceptibility to type IV hypersensitivity reaction
- reduced ear swelling in a contact hypersensitivity assay with oxazolone

increased interferon-gamma secretion
- Normal - however, lymph node cells treated with PMA and ionomycin exhibit normal secretion
- in a contact hypersensitivity assay with oxazolone

increased interleukin-6 secretion
- Normal - however, lymph node cells treated with PMA and ionomycin exhibit normal secretion
- in a contact hypersensitivity assay with oxazolone

increased tumor necrosis factor secretion
- Normal - however, lymph node cells treated with PMA and ionomycin exhibit normal secretion
- in a contact hypersensitivity assay with oxazolone

References

Baban B; Chandler P; McCool D; Marshall B; Munn DH; Mellor AL. 2004. Indoleamine 2,3-dioxygenase expression is restricted to fetal trophoblast giant cells during murine gestation and is maternal genome specific. J Reprod Immunol 61(2):67-77PubMed: 15063630 MGI: J:93797

Additional - Ido1^tm1Alm related

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR:Ido1^tm1Alm
- Genotyping resources and troubleshooting
Dietary Information
- LabDiet® 5K52 formulation (6% fat)
Breeding Considerations

When maintaining a live colony, these mice are bred as heterozygotes or homozygotes.
- Additional Breeding and Husbandry Support
Mating System
- Homozygote x Homozygote
Citation
When using the IDO KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #005867 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX10 (Standard)

Pricing & Availability

Available

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous for Indo<tm1Alm>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Related Products and Services

Frozen Mouse Embryo	B6.129-Ido1<tm1Alm>/J Frozen Embryos	$2595.00
Frozen Mouse Embryo	B6.129-Ido1<tm1Alm>/J Frozen Embryos	$2595.00
Frozen Mouse Embryo	B6.129-Ido1<tm1Alm>/J Frozen Embryos	$3373.50
Frozen Mouse Embryo	B6.129-Ido1<tm1Alm>/J Frozen Embryos	$3373.50

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.

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Animal Care and Use for SERVICES

Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.

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In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.

Also Known As: IDO KO

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Ido1tm1Alm

Allele Symbol: Ido1tm1Alm

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Ido1tm1Alm/Ido1tm1Almeither: (involves: 129X1/SvJ * C57BL/6) or (involves: 129X1/SvJ * CBA)

mammalian phenotype

Genotype: Ido1tm1Alm/Ido1tm1Alminvolves: 129X1/SvJ * BALB/c

cellular phenotype

immune system phenotype

mammalian phenotype

reproductive system phenotype

hematopoietic system phenotype

homeostasis/metabolism phenotype

Genotype: Ido1tm1Alm/Ido1tm1Alminvolves: 129X1/SvJ

mammalian phenotype

Genotype: Ido1tm1Alm/Ido1tm1Alminvolves: 129X1/SvJ * C57BL/6J * FVB/N

immune system phenotype

References

Additional - Ido1tm1Alm related

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Ido1^tm1Alm

Allele Symbol: Ido1^tm1Alm

Genotype: Ido1^tm1Alm/Ido1^tm1Alm
either: (involves: 129X1/SvJ * C57BL/6) or (involves: 129X1/SvJ * CBA)

Genotype: Ido1^tm1Alm/Ido1^tm1Alm
involves: 129X1/SvJ * BALB/c

Genotype: Ido1^tm1Alm/Ido1^tm1Alm
involves: 129X1/SvJ

Genotype: Ido1^tm1Alm/Ido1^tm1Alm
involves: 129X1/SvJ * C57BL/6J * FVB/N

Additional - Ido1^tm1Alm related