Overview

This targeted mutant was created and characterized by Deltagen, Inc. View phenotypic data developed by Deltagen.

Genetic overview

Genetic Background	Generation

Grk5^tm1Dgen

Allele Type	Gene Symbol	Gene Name
Targeted (Reporter, Null/Knockout)	Grk5	G protein-coupled receptor kinase 5

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Research Applications

Research Tools
Neurobiology Research

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Details

Detailed Description

This targeted mutant was created and characterized by Deltagen, Inc. View phenotypic data developed by Deltagen.

Development

The construct insert is reported to be "Lac0-SA-IRES-lacZ-Neo555G/Kan." Upon arrival at The Jackson Laboratory, these mice had been backcrossed at least 5 generations to C57BL/6 mice.

Expression Data

Expressed Gene	lacZ, beta-galactosidase, E. coli
Site of Expression	lacZ expression is detected in most organs examined: brain, spinal cord, sciatic nerve, eyes, thymus, spleen, lymph nodes, aorta, heart, lung, liver, pancreas, kidney, urinary bladder, thyroid gland, larynx, pituitary gland, adrenal glands, skeletal muscle, skin, testis, prostate gland, ovary, uterus and cervix. Strong expression is detected in the CNS, spleen, heart, lung and larynx.

Control Suggestions

Wild-type from the colony
000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Genetics

Grk5^tm1Dgen

Allele Symbol: Grk5^tm1Dgen

Allele Name	targeted mutation 1, Deltagen
Allele Type	Targeted (Reporter, Null/Knockout)
Allele Synonym(s)
Gene Symbol and Name	Grk5, G protein-coupled receptor kinase 5
Gene Synonym(s)
Expressed Gene	lacZ, beta-galactosidase, E. coli
Site of Expression	lacZ expression is detected in most organs examined: brain, spinal cord, sciatic nerve, eyes, thymus, spleen, lymph nodes, aorta, heart, lung, liver, pancreas, kidney, urinary bladder, thyroid gland, larynx, pituitary gland, adrenal glands, skeletal muscle, skin, testis, prostate gland, ovary, uterus and cervix. Strong expression is detected in the CNS, spleen, heart, lung and larynx.
Strain of Origin	129P2/OlaHsd
Chromosome	19
Molecular Note	A bacterial lacZ gene was inserted into the gene such that the endogenous gene promoter drives expression of beta-galactosidase. RT-PCR analysis detects gene transcript in all tissues tested. Beta-galactosidase expression from this allele is detectable in most tissues examined. Strongest staining was observed in the central nervous system, spleen, heart, lung and larynx. Staining was observed in different tissue and cell types, including smooth, cardiac and skeletal muscle, neurons, epithelium, cartilage, lymphatic tissue, adipose tissue and blood vessels.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- lacZ Expression
Neurobiology Research

Mammalian Phenotype Terms by Genotype

References

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Grk5
- Genotyping resources and troubleshooting
Citation
When using the B6.129P2-Grk5^tm1Dgen/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #005808 in your Materials and Methods section.

Overview

Genetic overview

Grk5tm1Dgen

Research Applications

Details

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Genetics

Grk5tm1Dgen

Allele Symbol: Grk5tm1Dgen

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Technical Support

Genotyping Protocols

Citation

Related Strains

Grk5^tm1Dgen

Grk5^tm1Dgen

Allele Symbol: Grk5^tm1Dgen