This targeted mutant was created and characterized by Deltagen, Inc. View phenotypic data developed by Deltagen.
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Grk5 | G protein-coupled receptor kinase 5 |
This targeted mutant was created and characterized by Deltagen, Inc. View phenotypic data developed by Deltagen.
The construct insert is reported to be "Lac0-SA-IRES-lacZ-Neo555G/Kan." Upon arrival at The Jackson Laboratory, these mice had been backcrossed at least 5 generations to C57BL/6 mice.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | lacZ expression is detected in most organs examined: brain, spinal cord, sciatic nerve, eyes, thymus, spleen, lymph nodes, aorta, heart, lung, liver, pancreas, kidney, urinary bladder, thyroid gland, larynx, pituitary gland, adrenal glands, skeletal muscle, skin, testis, prostate gland, ovary, uterus and cervix. Strong expression is detected in the CNS, spleen, heart, lung and larynx. |
Allele Name | targeted mutation 1, Deltagen |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Grk5, G protein-coupled receptor kinase 5 |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | lacZ expression is detected in most organs examined: brain, spinal cord, sciatic nerve, eyes, thymus, spleen, lymph nodes, aorta, heart, lung, liver, pancreas, kidney, urinary bladder, thyroid gland, larynx, pituitary gland, adrenal glands, skeletal muscle, skin, testis, prostate gland, ovary, uterus and cervix. Strong expression is detected in the CNS, spleen, heart, lung and larynx. |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 19 |
Molecular Note | A bacterial lacZ gene was inserted into the gene such that the endogenous gene promoter drives expression of beta-galactosidase. RT-PCR analysis detects gene transcript in all tissues tested. Beta-galactosidase expression from this allele is detectable in most tissues examined. Strongest staining was observed in the central nervous system, spleen, heart, lung and larynx. Staining was observed in different tissue and cell types, including smooth, cardiac and skeletal muscle, neurons, epithelium, cartilage, lymphatic tissue, adipose tissue and blood vessels. |
When using the B6.129P2-Grk5tm1Dgen/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #005808 in your Materials and Methods section.
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