Overview

This targeted mutant was created and characterized by Deltagen, Inc. View phenotypic data developed by Deltagen.

Genetic overview

Genetic Background	Generation

Allele Type	Gene Symbol	Gene Name
Targeted (Reporter, Null/Knockout)	Grk5	G protein-coupled receptor kinase 5

Research Applications

Research Tools
Neurobiology Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

This targeted mutant was created and characterized by Deltagen, Inc. View phenotypic data developed by Deltagen.

Development

The construct insert is reported to be "Lac0-SA-IRES-lacZ-Neo555G/Kan." Upon arrival at The Jackson Laboratory, these mice had been backcrossed at least 5 generations to C57BL/6 mice.

Expression Data

Expressed Gene	lacZ, beta-galactosidase, E. coli
Site of Expression	lacZ expression is detected in most organs examined: brain, spinal cord, sciatic nerve, eyes, thymus, spleen, lymph nodes, aorta, heart, lung, liver, pancreas, kidney, urinary bladder, thyroid gland, larynx, pituitary gland, adrenal glands, skeletal muscle, skin, testis, prostate gland, ovary, uterus and cervix. Strong expression is detected in the CNS, spleen, heart, lung and larynx.

Control Suggestions

Wild-type from the colony
000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Genetics

Grk5^tm1Dgen

Allele Symbol: Grk5^tm1Dgen

Allele Name	targeted mutation 1, Deltagen
Allele Type	Targeted (Reporter, Null/Knockout)
Allele Synonym(s)
Gene Symbol and Name	Grk5, G protein-coupled receptor kinase 5
Gene Synonym(s)
Expressed Gene	lacZ, beta-galactosidase, E. coli
Site of Expression	lacZ expression is detected in most organs examined: brain, spinal cord, sciatic nerve, eyes, thymus, spleen, lymph nodes, aorta, heart, lung, liver, pancreas, kidney, urinary bladder, thyroid gland, larynx, pituitary gland, adrenal glands, skeletal muscle, skin, testis, prostate gland, ovary, uterus and cervix. Strong expression is detected in the CNS, spleen, heart, lung and larynx.
Strain of Origin	129P2/OlaHsd
Chromosome	19
Molecular Note	A bacterial lacZ gene was inserted into the gene such that the endogenous gene promoter drives expression of beta-galactosidase. RT-PCR analysis detects gene transcript in all tissues tested. Beta-galactosidase expression from this allele is detectable in most tissues examined. Strongest staining was observed in the central nervous system, spleen, heart, lung and larynx. Staining was observed in different tissue and cell types, including smooth, cardiac and skeletal muscle, neurons, epithelium, cartilage, lymphatic tissue, adipose tissue and blood vessels.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- lacZ Expression
Neurobiology Research

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Grk5^tm1Dgen/Grk5^tm1Dgen
involves: 129P2/OlaHsd * C57BL/6

behavior/neurological phenotype

increased thermal nociceptive threshold
- homozygous mice exhibited a significant increase in thermal response latency during hot plate testing, indicating a possible increased pain threshold

integument phenotype

increased thermal nociceptive threshold
- homozygous mice exhibited a significant increase in thermal response latency during hot plate testing, indicating a possible increased pain threshold

References

Additional - Grk5^tm1Dgen related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Grk5
- Genotyping resources and troubleshooting
Citation
When using the B6.129P2-Grk5^tm1Dgen/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #005808 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous or Wild-type for Grk5<tm1Dgen>

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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Licensing Information

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Email: TechTran@jax.org