B6.129P2-Grk5^tm1Dgen/J

Stock number: 005808
Research areas: Neurobiology Research, Research Tools

Detailed description

This targeted mutant was created and characterized by Deltagen, Inc. View phenotypic data developed by Deltagen.

Development

The construct insert is reported to be "Lac0-SA-IRES-lacZ-Neo555G/Kan." Upon arrival at The Jackson Laboratory, these mice had been backcrossed at least 5 generations to C57BL/6 mice.

Allele

Symbol: Grk5^tm1Dgen
Name: targeted mutation 1, Deltagen
MGI accession ID: MGI:3604583
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Grk5
Marker name: G protein-coupled receptor kinase 5
Chromosome: 19
Strain of origin: 129P2/OlaHsd
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: lacZ expression is detected in most organs examined: brain, spinal cord, sciatic nerve, eyes, thymus, spleen, lymph nodes, aorta, heart, lung, liver, pancreas, kidney, urinary bladder, thyroid gland, larynx, pituitary gland, adrenal glands, skeletal muscle, skin, testis, prostate gland, ovary, uterus and cervix. Strong expression is detected in the CNS, spleen, heart, lung and larynx.
Molecular note: A bacterial lacZ gene was inserted into the gene such that the endogenous gene promoter drives expression of beta-galactosidase. RT-PCR analysis detects gene transcript in all tissues tested. Beta-galactosidase expression from this allele is detectable in most tissues examined. Strongest staining was observed in the central nervous system, spleen, heart, lung and larynx. Staining was observed in different tissue and cell types, including smooth, cardiac and skeletal muscle, neurons, epithelium, cartilage, lymphatic tissue, adipose tissue and blood vessels.