Overview

Also Known As: CB2R KO

CB₂ is normally up-regulated following pro-inflammatory and nociceptive stimuli, and in human brain tissues affected by Alzheimer's disease (AD), multiple sclerosis (MS) and amyotrophic lateral sclerosis (ALS). These CB₂ knockout mice (CB₂^-, CB₂R^- or Cnr2^-) may be useful for studying these diseases, pain management and drug/antibody therapeutics.

Genetic overview

Genetic Background	Generation
	?+N2F9 (2019-06-13 00:00:00)

Cnr2^tm1Dgen

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Cnr2	cannabinoid receptor 2 (macrophage)

View Genetics

Research Applications

Neurobiology Research
Immunology, Inflammation and Autoimmunity Research

View All Research Applications

Base Price

Starting at:

$236.78 Domestic price for female

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Details

Detailed Description

Cannabinoid receptor 2 (CB₂ or CB₂R) is normally expressed in cells of the haematopoietic lineage, central nervous system and bone. In immune cells, CB₂ is expressed on leukocytes and localized in intracellular membranous structures. Endogenous expression is up-regulated by pro-inflammatory and nociceptive stimuli. CB₂ activation reduces expression of adhesion molecules in brain endothelium, decreasing leukocyte engagement and recruitment.

In these CB₂ knockout mice (CB₂^-/-, CB₂R^-/-, Cnr2^-/- or Cnr2^tm1Dgen), CB₂ agonists have no effect on minimizing leukocyte-endothelial cell interaction. After treatment with lipopolysaccharide (LPS), CB₂ null mice fail to show complete resolution of inflammation compared to wild type controls. An increase in leukocyte adhesion is seen when Cnr2^-/- leukocytes are transferred into wildtype recipient mice.

This CB₂ knockout mutant was created and characterized by Deltagen, Inc. View phenotypic data developed by Deltagen.

J20 transgenic amyloid mice express the K670N/M671L/V717F mutant form of amyloid precursor protein under control of the human platelet–derived growth factor β polypeptide promoter (Stock No. 006293). Hemizygous J20 mice are a Familial Alzheimer's Disease model with progressive amyloid deposition as they age (significant reactive microgliosis by 8 months of age). C57BL/6 mice homozygous for Cnr2^tm1Dgen and also harboring the J20 transgene (J20 CNR2^/-/) exhibit increased amyloid pathology (increased soluble Aβ42, plaque deposition and plaque associated microglia), but altered tau processing (suppressed total tau), when compared to J20 mice alone.

Of note, another published CB₂ knockout allele (Cnr2^tm1Zim) has been used to show CB₂-deficient macrophages are resistant to the inhibitory effects of δ-9 tetrahydrocannabinol (THC) and CB₂ knockout mice have increased severity in experimental autoimmune encephalitis. Homozygous Cnr2^tm1Zim mice also harboring the R6/2 transgene (encoding human mutant Huntington exon 1) have increased microglial activation and exacerbated Huntington disease progression/excitotoxicity compared to R6/2 mice alone.

Development

The CB₂ knockout allele (CB₂^-, CB₂R^-, Cnr2^- or Cnr2^tm1Dgen) was created by Deltagen. Specifically, the "Neo555T" construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Targeted mutant cell line 655 had homologous recombination of the "Neo555T" construct resulting in a 334 bp deletion in the coding exon of cannabinoid receptor 2 [macrophage] locus (CB₂; Cnr2) on chromosome 4. The donating investigator reported the Cnr2^- mice were backcrossed at least five generations to C57BL/6 mice (see SNP note below) prior to sending to The Jackson Laboratory Repository.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 2 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Control Suggestions

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Genetics

Cnr2^tm1Dgen

Allele Symbol: Cnr2^tm1Dgen

Allele Name	targeted mutation 1, Deltagen
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	Cnr2^tm1Dgen; targeted mutation 1, Deltagen
Gene Symbol and Name	Cnr2, cannabinoid receptor 2 (macrophage)
Gene Synonym(s)	CX5; CB-2; CB2; CB2C; CNR2C; cannabinoid receptor 2 (spleen); CB2-R
Strain of Origin	129P2/OlaHsd
Chromosome	4
Molecular Note	This gene was disrupted by homologous recombination. Normally, RNA transcripts are detectable in lung, liver, thymus, spleen, lymph nodes, bone marrow, skin and testis.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Receptor Defects
  - cannabinoid receptor
Immunology, Inflammation and Autoimmunity Research

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Cnr2^tm1Dgen/Cnr2^tm1Dgen
involves: 129P2/OlaHsd * C57BL/6

integument phenotype

alopecia
- in three out of seven homozygotes

References

Additional - Cnr2^tm1Dgen related

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR: Cnr2^tm1Dgen
- Genotyping resources and troubleshooting
Dietary Information
- LabDiet® 5K52 formulation (6% fat)
Breeding Considerations

When maintaining a live colony, heterozygous or homozygous mice may be bred together.
- Additional Breeding and Husbandry Support
Mating System
- Homozygote x Homozygote
Citation
When using the CB2R KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #005786 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX11 (Maximum)

Pricing & Availability

Available

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or Wild-type for Cnr2<tm1Dgen>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

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Licensing Information

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Email: TechTran@jax.org

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The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

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Also Known As: CB2R KO

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Additional Information

Cnr2tm1Dgen

Allele Symbol: Cnr2tm1Dgen

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Cnr2tm1Dgen/Cnr2tm1Dgeninvolves: 129P2/OlaHsd * C57BL/6

integument phenotype

References

Additional - Cnr2tm1Dgen related

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Cnr2^tm1Dgen

Allele Symbol: Cnr2^tm1Dgen

Genotype: Cnr2^tm1Dgen/Cnr2^tm1Dgen
involves: 129P2/OlaHsd * C57BL/6

Additional - Cnr2^tm1Dgen related