These mice express a transgene containing the protein coding region of mouse preproinsulin II (Ins2). They do not develop spontaneous diabetes and are resistant to cyclophosphamide-induced diabetes.
Leonard Harrison, Walter and Eliza Hall Institute of Medic
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Inserted expressed sequence) |
Transgenic mice are viable, fertile, normal in size, normoglycemic and do not display any gross physical or behavioral abnormalities. RT/PCR indicates transgene expression in the spleen and thymus. Blood glucose levels of transgenic and non-transgenic littermates are identical. 90-98 % of the transgenic islets of females and males are insulitis free. Transgenic mice do not develop spontaneous diabetes and are resistant to cyclophosphamide-induced diabetes. Sialitis is not statistically different between transgenic and littermate controls (French MB, Diabetes 1997 46:34-9). Transgenic bone marrow transplanted into 4-week-old irradiated NOD females almost entirely prevented diabetes compared to control NOD to irradiated NOD transplants, which have an overall diabetes incidence similar to untreated controls. Thymoma incidence was similar in both irradiated groups.(Steptoe RJ, J Clin Invest 2003 111:1357-63)
This model may be helpful for looking at antigen specific immunotherapeutic strategies for preventing T1D and other autoimmune diseases.
The H2-Ea-Ins2 transgene encodes the protein coding region of mouse preproinsulin II (Ins2) amplified from genomic NOD DNA, controlled by an MHC class II, I-E alphak, promoter. The transgenic fragment was directly injected into NOD/LtWehi oocytes and has been maintained on an NOD/LtWehi background. In 2005, the T1DR received NOD-Tg(H2-Ea-Ins2)1Wehi/WehiJ transgenic mice and mated to NOD/LtJ prior to mating Tg x Tg.
Expressed Gene | Ins2, insulin II, mouse, laboratory |
---|---|
Site of Expression |
Allele Name | transgene insertion 1, William and Eliza Hall Institute |
---|---|
Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(H2-Ea-Ins2)1Wehi, transgene insertion 1, William and Eliza Hall Institute |
Gene Synonym(s) | |
Promoter | H2-I, H2 I region, includes H2-Aa, Ab, Bl, Ea, Eb, Eb2, Ob, Pb, mouse, laboratory |
Expressed Gene | Ins2, insulin II, mouse, laboratory |
Strain of Origin | NOD/ShiLt |
Chromosome | UN |
General Note | Two lines of mice were created with this trasgenic construct on the NOD background. No distinction was made between them. |
Molecular Note | The transgene comprises nucleotides -1,903 through -39 of the 5' flanking sequence of the MHC Class II gene H2-Ea (k allele), containing the promoter, followed by a 909 base pair mouse (NOD strain) genomic DNA fragment containing the sequence encoding prepronsulin II and the SV40 small t intron and polyadenylation signal. Transgene-derived mRNA is detectable in spleen and thymus of transgenic NOD/LtWehi mice, but not of control littermates, by reverse transcription followed by PCR (RT/PCR) using either primers for H2-Eak and proinsulin or two proinsulin primers. Immunoreactive proinsulin is detectable in medium from cultured transgenic splenocytes only after 8 days' culture and at levels 10-2 - 10-3 times those of cultured islets or islet cells; levels are increased by culture with interferon gamma and tumor necrosis factor alpha. |
Mutations Made By | Leonard Harrison, Walter and Eliza Hall Institute of Medic |
When using the NOD.MHCII-Ins2 mouse strain in a publication, please cite the originating article(s) and include JAX stock #005739 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(H2-Ea-Ins2)1Wehi |
Frozen Mouse Embryo | NOD-Tg(H2-Ea-Ins2)1Wehi/WehiJ Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | NOD-Tg(H2-Ea-Ins2)1Wehi/WehiJ Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | NOD-Tg(H2-Ea-Ins2)1Wehi/WehiJ Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | NOD-Tg(H2-Ea-Ins2)1Wehi/WehiJ Frozen Embryos | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.