| Allele Name | transgene insertion 1, William and Eliza Hall Institute |
| Allele Type | Transgenic (Inserted expressed sequence) |
| Allele Synonym(s) | |
| Gene Symbol and Name | Tg(H2-Ea-Ins2)1Wehi, transgene insertion 1, William and Eliza Hall Institute |
| Gene Synonym(s) | |
| Promoter | H2-I, H2 I region, includes H2-Aa, Ab, Bl, Ea, Eb, Eb2, Ob, Pb, mouse, laboratory |
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| Expressed Gene | Ins2, insulin II, mouse, laboratory |
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| Strain of Origin | NOD/ShiLt |
| Chromosome | UN |
| General Note | Two lines of mice were created with this trasgenic construct on the NOD background. No distinction was made between them. |
| Molecular Note | The transgene comprises nucleotides -1,903 through -39 of the 5' flanking sequence of the MHC Class II gene H2-Ea (k allele), containing the promoter, followed by a 909 base pair mouse (NOD strain) genomic DNA fragment containing the sequence encoding prepronsulin II and the SV40 small t intron and polyadenylation signal. Transgene-derived mRNA is detectable in spleen and thymus of transgenic NOD/LtWehi mice, but not of control littermates, by reverse transcription followed by PCR (RT/PCR) using either primers for H2-Eak and proinsulin or two proinsulin primers. Immunoreactive proinsulin is detectable in medium from cultured transgenic splenocytes only after 8 days' culture and at levels 10-2 - 10-3 times those of cultured islets or islet cells; levels are increased by culture with interferon gamma and tumor necrosis factor alpha. |
| Mutations Made By | Leonard Harrison, Walter and Eliza Hall Institute of Medic |
