B6.FVB-Tg(tetO-EGFP,-Tgfbr2)8Mcle/J

Stock number: 005738
Product name: PTR
Research areas: Research Tools

Description

These PTR mice express a transgene containing EGFP and a truncated dominant-negative mouse transforming growth factor, beta receptor II (Tgfbr2) and when bred with mice expressing rtTA or tTA, tissue-specific EGFP and dominant-negative Tgfbr2 transgene expression can be regulated with doxycycline.

Detailed description

Mice homozygous for the transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Enhanced Green Fluorescent Protein (EGFP) and a truncated dominant-negative mouse transforming growth factor, beta receptor II (Tgfbr2) under the control of a bidirectional tetracycline transactivator responsive promoter (TRE; tetO). No fluorescence is observed in the tissues of this mutant. Primary cultures derived from ear biopsies exhibit green fluorescence when transfected with a tetracycline transactivator (tTA; "Tet-off") vector. When these transgenic mice are bred with other transgenic mice expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), tissue-specific EGFP and dominant-negative Tgfbr2 transgene expression can be regulated with the tetracycline analog, doxycycline. This mutant mouse strain may be useful in studies of TGF-beta signaling.

Development

A transgenic construct containing an Enhanced Green Fluorescent Protein gene and a truncated mouse transforming growth factor, beta receptor II gene under the control of the a bidirectional tetracycline-responsive promoter (TRE; tetO) was microinjected into fertilized FVB/N oocytes. Founder line 8 (H) was subsequently established. The mice were then backcrossed to the C57BL/6 background for 5 generations.

Allele

Symbol: Tg(tetO-EGFP,-Tgfbr2)8Mcle
Name: transgene insertion 8, Ian S McLennan
MGI accession ID: MGI:3691946
Generation method: Transgenic
Attributes: Reporter; Inducible; Dominant negative; Inserted expressed sequence
Synonyms: PTR; PTR (line 8); PTRh
Marker symbol: Tg(tetO-EGFP,-Tgfbr2)8Mcle
Marker name: transgene insertion 8, Ian S McLennan
Marker synonyms: PTR; PTR (line 8); PTRh
Chromosome: UN
Strain of origin: FVB/N
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: Primary cultures derived from ear biopsies exhibit green fluorescence when transfected with a tetracycline transactivator (Tet-off) vector. When bred with another transgenic mouse expressing rtTA or tTA to create a bitransgenic animal, tissue specific EGFP & dominant-negative mouse mouse transforming growth factor, beta receptor II transgene expression can be regulated with the tetracy
Molecular note: The transgene comprises a bidirectional tetracycline transactivator responsive promoter (tetO) between an enhanced green fluorescent protein gene with a polyadenylation (poly[A]) signal derived from simian virus 40 (SV40) and a truncated transforming growth factor, beta receptor II cDNA with a c-Myc epitope tag and a beta-globin gene-derived poly[A] signal. The encoded TGFBR2 protein, which lacks the intracellular kinase domain, functions as a dominant-negative receptor, disrupting TGFbeta signaling. Tissues of transgenic mice do not fluoresce. Coordinate expression of EGFP and dominant-negative TGFBR2 can be regulated in a tissue-specific manner in bitransgenic mice with this and a tetracycline transactivator (tTA; "tet-off") or reverse transactivator (rtTA; "tet-on") transgene by administration/withdrawal of the tetracycline analog doxycycline.
General note: Of 17 transgenic mice derived from microinjected oocytes, 6 yielded cell cultures that strongly expressed EGFP upon transfection with a tetracycline transactivator- (tTA-; "tet-off") expressing plasmid. Offspring of two transgenic founder lines, designated by the authors PTRe and PTRh, by tTA transgenic mice were subjected to further expression analysis.