These PTR mice express a transgene containing EGFP and a truncated dominant-negative mouse transforming growth factor, beta receptor II (Tgfbr2) and when bred with mice expressing rtTA or tTA, tissue-specific EGFP and dominant-negative Tgfbr2 transgene expression can be regulated with doxycycline.
Ian McLennan, University of Otago
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter, Inducible, Dominant negative, Inserted expressed sequence) |
Mice homozygous for the transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Enhanced Green Fluorescent Protein (EGFP) and a truncated dominant-negative mouse transforming growth factor, beta receptor II (Tgfbr2) under the control of a bidirectional tetracycline transactivator responsive promoter (TRE; tetO). No fluorescence is observed in the tissues of this mutant. Primary cultures derived from ear biopsies exhibit green fluorescence when transfected with a tetracycline transactivator (tTA; "Tet-off") vector. When these transgenic mice are bred with other transgenic mice expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), tissue-specific EGFP and dominant-negative Tgfbr2 transgene expression can be regulated with the tetracycline analog, doxycycline. This mutant mouse strain may be useful in studies of TGF-beta signaling.
A transgenic construct containing an Enhanced Green Fluorescent Protein gene and a truncated mouse transforming growth factor, beta receptor II gene under the control of the a bidirectional tetracycline-responsive promoter (TRE; tetO) was microinjected into fertilized FVB/N oocytes. Founder line 8 (H) was subsequently established. The mice were then backcrossed to the C57BL/6 background for 5 generations.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | Primary cultures derived from ear biopsies exhibit green fluorescence when transfected with a tetracycline transactivator (Tet-off) vector. When bred with another transgenic mouse expressing rtTA or tTA to create a bitransgenic animal, tissue specific EGFP & dominant-negative mouse mouse transforming growth factor, beta receptor II transgene expression can be regulated with the tetracy |
Allele Name | transgene insertion 8, Ian S McLennan |
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Allele Type | Transgenic (Reporter, Inducible, Dominant negative, Inserted expressed sequence) |
Allele Synonym(s) | PTR; PTR (line 8); PTRh |
Gene Symbol and Name | Tg(tetO-EGFP,-Tgfbr2)8Mcle, transgene insertion 8, Ian S McLennan |
Gene Synonym(s) | |
Promoter | tetO, tet operator, |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | Primary cultures derived from ear biopsies exhibit green fluorescence when transfected with a tetracycline transactivator (Tet-off) vector. When bred with another transgenic mouse expressing rtTA or tTA to create a bitransgenic animal, tissue specific EGFP & dominant-negative mouse mouse transforming growth factor, beta receptor II transgene expression can be regulated with the tetracy |
Strain of Origin | FVB/N |
Chromosome | UN |
General Note | Of 17 transgenic mice derived from microinjected oocytes, 6 yielded cell cultures that strongly expressed EGFP upon transfection with a tetracycline transactivator- (tTA-; "tet-off") expressing plasmid. Offspring of two transgenic founder lines, designated by the authors PTRe and PTRh, by tTA transgenic mice were subjected to further expression analysis. |
Molecular Note | The transgene comprises a bidirectional tetracycline transactivator responsive promoter (tetO) between an enhanced green fluorescent protein gene with a polyadenylation (poly[A]) signal derived from simian virus 40 (SV40) and a truncated transforming growth factor, beta receptor II cDNA with a c-Myc epitope tag and a beta-globin gene-derived poly[A] signal. The encoded TGFBR2 protein, which lacks the intracellular kinase domain, functions as a dominant negative receptor, disrupting TGFbeta signaling. Tissues of transgenic mice do not fluoresce. Coordinate expression of EGFP and dominant-negative TGFBR2 can be regulated in a tissue-specific manner in bitransgenic mice with this and a tetracycline transactivator (tTA; "tet-off") or reverse transactivator (rtTA; "tet-on") transgene by administration/withdrawal of the tetracycline analog doxycycline. |
Mutations Made By | Ian McLennan, University of Otago |
When maintaining a live colony, these mice are bred as homozygotes.
When using the PTR mouse strain in a publication, please cite the originating article(s) and include JAX stock #005738 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(tetO-EGFP,-Tgfbr2)8Mcle |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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