NOD/ShiLt-Tg(Ins2-Fas*I246N)1Ach/AchJ

Stock number: 005733
Product name: NOD.RIP-Fas^cg
Research areas: Diabetes and Obesity Research

Description

These NOD.RIP-Fas^cg mice have a beta cell-specific expression of a dominant-negative point mutation of Fas.

Detailed description

Diabetes onset is significantly delayed in Rip-Fas^cg transgenic mice (45% diabetic by 30 weeks of age) compared to wildtype cohorts (90% diabetic by 30 weeks of age). Adoptive transfer studies using splenocytes from diabetic NOD/Lt mice into Rip-Fas^cg transgenic mice indicate the transgenic mice become diabetic later than wildtype controls. This transgenic strain is useful in studies looking at the effects of Fas on pancreatic islets.

Development

Fas cDNA with a single nucleotide change leading to a I246N change in protein sequence, which codes for Fas^cg was subcloned into the rat insulin 2 promoter and injected into NOD/Lt oocytes. In 2006, the T1DR received mice at generation N?F8.

Allele

Symbol: Tg(Ins2-Fas*I246N)1Ach
Name: transgene insertion 1, Alexander V Chervonsky
MGI accession ID: MGI:3662920
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Ins2-Fas*I246N)1Ach
Marker name: transgene insertion 1, Alexander V Chervonsky
Chromosome: UN
Strain of origin: NOD/ShiLt
Expressed genes: Fas,Fas cell surface death receptor,rat
Molecular note: Fas cDNA with a single nucleotide change leading to a I246N change in protein sequence, which codes for Fas^cg was subcloned into the rat insulin 2 promoter and injected into NOD/Lt oocytes to produce transgenic mice.