These Tie2lacZ+/RAG1- mice express a transgene containing lacZ and Tek (formerly Tie2). They are also immunodeficient as a result of the Rag1 KO.
Brenda Coomber, University of Guelph
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Rag1 | recombination activating gene 1 |
Allele Type |
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Transgenic (Reporter) |
Mice homozygous for both the targeted mutation and the transgene are viable and fertile. The transgene beta-galactosidase is well expressed, reflects endogenous Tek/Tie2 activity, and is specific to the vascular endothelium. Mice homozygous for the Rag1 mutation are immunodeficient as they lack mature B and T lymphocytes. This double mutant mouse may be useful in studies of cancer, tumor-related angiogenesis, and as a recipient of xenografted tumors.
The transgene consists of a beta-galactosidase reporter gene under the control of a modified murine endothelial-specific receptor tyrosine kinase (Tek/Tie2) promoter. Targeted mutation of recombination activating gene-1 (Rag1) resulted in a 1356 base pair deletion in the 5' end of the endogenous coding sequence and PGK-Neo cassette insertion. Double mutant mice were generated by crossing homozygous female B6.129S7-Rag1tm1Mom/J (see Stock No. 002216) with hemizygous male FVB/N-Tg(TIE2-lacZ)182Sato/J (see Stock No. 002856) mice. Female progeny were then mated to males homozygous for the Rag1 mutation to fix Rag1-deficiency. Subsequent sibling intercross resulted in presumed homozygosity of the transgene on chromosome X (via test crosses to wildtype). Other transgene insertion points on autosomes are likely.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | lacZ expression is detected in endothelial cells. |
Allele Name | targeted mutation 1, Peter Mombaerts |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Rag-; Rag1tm1Mom; RAG-1-; Rag1-; RAG1null; Rag-1KO |
Gene Symbol and Name | Rag1, recombination activating gene 1 |
Gene Synonym(s) | |
Site of Expression | expression is seen in bone marrow derived cell lines. |
Strain of Origin | 129S7/SvEvBrd-Hprt+ |
Chromosome | 2 |
Molecular Note | A 1356 bp genomic fragment of the Rag1 gene, encoding the nuclear localization signal and the zinc-finger motif, was replaced by a neomycin cassette. A mutant transcript expressed from this allele was detected by Northern blot in bone marrow derived cell lines from homozygous mice. |
Mutations Made By | Peter Mombaerts, Max Planck Research Unit for Neurogenetics |
Allele Name | transgene insertion 182, Thomas N Sato |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | Tg(TIE2LacZ)182Sato; Tg182Sato; Tie2/LacZ; Tie2-lacZ; TIE2p/e-LacZ |
Gene Symbol and Name | Tg(TIE2-lacZ)182Sato, transgene insertion 182, Thomas N Sato |
Gene Synonym(s) | |
Promoter | Tek, TEK receptor tyrosine kinase, mouse, laboratory |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | lacZ expression is detected in endothelial cells. |
Strain of Origin | Not Specified |
Chromosome | UN |
General Note | In embryonic and adult transgenic mice on an FVB/N background, LacZ is expressed specifically in vascular endothelial cells. |
Molecular Note | The transgene consists of a mouse Tek promoter and enhancer element, a lacZ reporter gene, and an SV40 polyadenylation sequence. The enhancer element is a genomic fragment extending from 3' of exon 1 to 10 kb into intron 1. Expression was driven in endothelial cells. |
Mutations Made By | Thomas Sato, Nara Institute of Science and Technology |
When maintaining a live colony, these mice are bred as homozygous for both the targeted mutation and the transgene. Specific pathogen free conditions are recommended as these mice are immunodeficient (lack mature B and T lymphocytes). Donating investigator reports occasional abnormalities at their facility; mice can agressively barber their cagemates, have tooth malocclusion at weaning, and a few develop 'crystal pneumonitis'.
When using the Tie2lacZ+/RAG1- mouse strain in a publication, please cite the originating article(s) and include JAX stock #005707 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Homozygous for Rag1<tm1Mom>, Homozygous for Tg(TIE2-lacZ)182Sato |
Frozen Mouse Embryo | STOCK Rag1<tm1Mom> Tg(TIE2-lacZ)182Sato/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | STOCK Rag1<tm1Mom> Tg(TIE2-lacZ)182Sato/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | STOCK Rag1<tm1Mom> Tg(TIE2-lacZ)182Sato/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | STOCK Rag1<tm1Mom> Tg(TIE2-lacZ)182Sato/J Frozen Embryos | $3373.50 |
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