STOCK Rag1^tm1Mom Tg(TIE2-lacZ)182Sato/J

Stock number: 005707
Product name: Tie2lacZ+/RAG1-
Research areas: Cancer Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Research Tools

Description

These Tie2lacZ+/RAG1- mice express a transgene containing lacZ and Tek (formerly Tie2). They are also immunodeficient as a result of the Rag1 KO.

Detailed description

Mice homozygous for both the targeted mutation and the transgene are viable and fertile. The transgene beta-galactosidase is well expressed, reflects endogenous Tek/Tie2 activity, and is specific to the vascular endothelium. Mice homozygous for the Rag1 mutation are immunodeficient as they lack mature B and T lymphocytes. This double mutant mouse may be useful in studies of cancer, tumor-related angiogenesis, and as a recipient of xenografted tumors.

Development

The transgene consists of a beta-galactosidase reporter gene under the control of a modified murine endothelial-specific receptor tyrosine kinase (Tek/Tie2) promoter. Targeted mutation of recombination activating gene-1 (Rag1) resulted in a 1356 base pair deletion in the 5' end of the endogenous coding sequence and PGK-Neo cassette insertion. Double mutant mice were generated by crossing homozygous female B6.129S7-Rag1^tm1Mom/J (see Stock No. 002216) with hemizygous male FVB/N-Tg(TIE2-lacZ)182Sato/J (see Stock No. 002856) mice. Female progeny were then mated to males homozygous for the Rag1 mutation to fix Rag1-deficiency. Subsequent sibling intercross resulted in presumed homozygosity of the transgene on chromosome X (via test crosses to wildtype). Other transgene insertion points on autosomes are likely.

Allele

Symbol: Tg(TIE2-lacZ)182Sato
Name: transgene insertion 182, Thomas N Sato
MGI accession ID: MGI:2449204
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(TIE2-lacZ)182Sato
Marker name: transgene insertion 182, Thomas N Sato
Chromosome: UN
Strain of origin: Not Specified
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: lacZ expression is detected in endothelial cells.
Molecular note: The transgene consists of a mouse Tek promoter (2.1 kb HindIII segment including most of exon1), a lacZ reporter gene, a Tek1 enhancer element, and an SV40 polyadenylation sequence. The enhancer element is 1.7 kb genomic sequence (XhoI-KpnI restriction fragment) from inside the 5' half of intron 1. Expression was driven in endothelial cells.
General note: In embryonic and adult transgenic mice on an FVB/N background, LacZ is expressed specifically in vascular endothelial cells.

Allele

Symbol: Rag1^tm1Mom
Name: targeted mutation 1, Peter Mombaerts
MGI accession ID: MGI:1857241
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Rag1
Marker name: recombination activating gene 1
Chromosome: 2
Strain of origin: 129S7/SvEvBrd-Hprt⁺
Site of expression: expression is seen in bone marrow derived cell lines.
Molecular note: A 1356 bp genomic fragment of the Rag1 gene, encoding the nuclear localization signal and the zinc-finger motif, was replaced by a neomycin cassette. A mutant transcript expressed from this allele was detected by Northern blot in bone marrow derived cell lines from homozygous mice.