C57BL/6-Tg(tetO-CDK5R1/GFP)337Lht/J

Stock number: 005706
Product name: p25 Tg
Research areas: Developmental Biology Research, Mouse/Human Gene Homologs, Neurobiology Research, Research Tools

Description

These p25 Tg mice express a transgene containing the CDK5R1/GFP fusion protein under the regulation of tetO.

Detailed description

Hemizygous transgenic mice are viable, fertile, normal in size and do not display any behavioral abnormalities. Mice homozygous for this transgene may not be viable. When these transgenic mice are bred with mice expressing the tetracycline-controlled transactivator protein (tTA) under the regulation of a tissue-specific promoter, expression of the CDK5R1/GFP fusion protein in the appropriate tissue of the bitransgenic offspring can be regulated by doxycycline administration. These mice may be useful in studies of Alzheimer's disease and other neurodegenerative tauopathies, amyotrophic lateral sclerosis (ALS), Niemann Pick Type C (NPC) disease, and Parkinson's disease.

Note: this transgenic strain was designed to breed with Tg(Camk2a-tTA) transgenic mice, (Stock No. 003010), a transgenic strain that expresses tTA in forebrain neurons. The resulting bitransgenic offspring exhibit the hallmark phenotype of Alzheimer's disease; elevated p25/Cdk5 related hyperphosporylation of tau leading to compromised tau function and progressive tau aggregation.

Development

A transgenic vector was generated encoding the carboxy terminal proteolytic fragment (p25) of human cyclin-dependent kinase 5, regulatory subunit 1 sequence (CDK5R1) bearing a carboxy-terminal green fluorescent protein (GFP) tag under control of a tetracycline-responsive promoter element (TRE; tetO). The construct was microinjected into C57BL/6J fertilized embryos which were implanted into FVB foster mothers. Male offspring were bred to C57BL/6J females, and transgene-positive mice (line 337) were backcrossed for more than 20 generations before arrival at The Jackson Laboratory.

Allele

Symbol: Tg(tetO-CDK5R1/GFP)337Lht
Name: transgene insertion 337, Li-Huei Tsai
MGI accession ID: MGI:3653679
Generation method: Transgenic
Attributes: Reporter; Inducible; Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(tetO-CDK5R1/GFP)337Lht
Marker name: transgene insertion 337, Li-Huei Tsai
Chromosome: UN
Strain of origin: C57BL/6J
Expressed genes: CDK5R1,cyclin dependent kinase 5 regulatory subunit 1,human; GFP,Green Fluorescent Protein,
Site of expression: When bred with mice having the tetracycline-controlled transactivator protein (tTA), this transgene can be inducibly expressed in the offspring. GFP acts as a fusion protein tag with CDK5R1 expression products.
Molecular note: The transgene expresses the carboxy-terminal proteolytic fragment (p25) of human cyclin-dependent kinase 5, regulatory subunit 1, bearing a carboxy-terminal green fluorescent protein (GFP) tag, under control of the tetracycline operator. The purpose of the GFP tag is to differentiate immunologically the transgene-encoded p25 from the endogenous p35 protein.