These p25 Tg mice express a transgene containing the CDK5R1/GFP fusion protein under the regulation of tetO.
Li-Huei Tsai, Massachusetts Institue of Technology
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter, Inducible, Inserted expressed sequence, Humanized sequence) |
Hemizygous transgenic mice are viable, fertile, normal in size and do not display any behavioral abnormalities. Mice homozygous for this transgene may not be viable. When these transgenic mice are bred with mice expressing the tetracycline-controlled transactivator protein (tTA) under the regulation of a tissue-specific promoter, expression of the CDK5R1/GFP fusion protein in the appropriate tissue of the bitransgenic offspring can be regulated by doxycycline administration. These mice may be useful in studies of Alzheimer's disease and other neurodegenerative tauopathies, amyotrophic lateral sclerosis (ALS), Niemann Pick Type C (NPC) disease, and Parkinson's disease.
Note: this transgenic strain was designed to breed with Tg(Camk2a-tTA) transgenic mice, (Stock No. 003010), a transgenic strain that expresses tTA in forebrain neurons. The resulting bitransgenic offspring exhibit the hallmark phenotype of Alzheimer's disease; elevated p25/Cdk5 related hyperphosporylation of tau leading to compromised tau function and progressive tau aggregation.
A transgenic vector was generated encoding the carboxy terminal proteolytic fragment (p25) of human cyclin-dependent kinase 5, regulatory subunit 1 sequence (CDK5R1) bearing a carboxy-terminal green fluorescent protein (GFP) tag under control of a tetracycline-responsive promoter element (TRE; tetO). The construct was microinjected into C57BL/6J fertilized embryos which were implanted into FVB foster mothers. Male offspring were bred to C57BL/6J females, and transgene-positive mice (line 337) were backcrossed for more than 20 generations before arrival at The Jackson Laboratory.
Expressed Gene | CDK5R1, cyclin dependent kinase 5 regulatory subunit 1, human |
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Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | When bred with mice having the tetracycline-controlled transactivator protein (tTA), this transgene can be inducibly expressed in the offspring. GFP acts as a fusion protein tag with CDK5R1 expression products. |
Allele Name | transgene insertion 337, Li-Huei Tsai |
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Allele Type | Transgenic (Reporter, Inducible, Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | CK-p25 Tg; p25(337); p25-CK; p25-GFP; tetO-p25-GFP; Tg(tetO-p25/GFP)33Lth |
Gene Symbol and Name | Tg(tetO-CDK5R1/GFP)337Lht, transgene insertion 337, Li-Huei Tsai |
Gene Synonym(s) | |
Promoter | tetO, tet operator, |
Expressed Gene | CDK5R1, cyclin dependent kinase 5 regulatory subunit 1, human |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | When bred with mice having the tetracycline-controlled transactivator protein (tTA), this transgene can be inducibly expressed in the offspring. GFP acts as a fusion protein tag with CDK5R1 expression products. |
Strain of Origin | C57BL/6J |
Chromosome | UN |
Molecular Note | The transgene expresses the carboxy-terminal proteolytic fragment (p25) of human cyclin-dependent kinase 5, regulatory subunit 1, bearing a carboxy-terminal green fluorescent protein (GFP) tag, under control of the tetracycline operator. The purpose of the GFP tag is to differentiate immunologically the transgene-encoded p25 from the endogenous p35 protein. |
Mutations Made By | Li-Huei Tsai, Massachusetts Institue of Technology |
When maintaining a live colony, transgenic positive siblings are bred. Alternatively, transgenic mice can be bred with C57BL/6J (Stock No. 000664). Transgenic homozygosity may be lethal.
When using the p25 Tg mouse strain in a publication, please cite the originating article(s) and include JAX stock #005706 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(tetO-CDK5R1/GFP)337Lht |
Frozen Mouse Embryo | C57BL/6-Tg(tetO-CDK5R1/GFP)337Lht/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(tetO-CDK5R1/GFP)337Lht/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(tetO-CDK5R1/GFP)337Lht/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Tg(tetO-CDK5R1/GFP)337Lht/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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