These Galr1 knock-out mice do not exhibit seizure behavior seen earlier in the development of this strain.
John Shine, Garvan Institute of Medical Research
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical abnormalities. No gene product (mRNA) is detected by RT-PCR analysis of mRNA from brain, spinal cord and uterus tissue. Although galanin receptor 1 targeted mutant mice on an incipient congenic B6.129S(N6) genetic background have been described to sometimes exhibit spontaneous seizures triggered by handling or bright light conditions, the donating investigator indicates that this mouse strain does not exhibit seizure behavior.
A targeting vector containing neomycin resistance gene was used to disrupt part of exon 1. The construct was electroporated into 129S1/SvImJ derived W9.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to the same for 13 generations.
|Allele Name||targeted mutation 1, Tiina Pauline Iismaa|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||GAL-R1-; Galr1-|
|Gene Symbol and Name||Galr1, galanin receptor 1|
|Strain of Origin||129S1/Sv-Oca2+ Tyr+ Kitl+|
|Molecular Note||A neomycin selection cassette was inserted 780 bp into the endogenous locus via homologous recombination. Normal transcript was undetected in homozygous mutant mice by RT-PCR analysis.|
|Mutations Made By|| |
Arie Jacoby, Victor Chang Cardiac Research Institute
When maintaining a live colony, these mice are bred as homozygotes.
When using the Galr1- mouse strain in a publication, please cite the originating article(s) and include JAX stock #005695 in your Materials and Methods section.