In this targeted reporter strain, the Cxcr6 coding region was replaced by EGFP cDNA. EGFP is expressed on liver leukocytes, but is not seen on other leukocytes like macrophages and neutrophils. EGFP expression is seen on natural killer T (NKT) cells of the liver. This mutant may be useful in studies of hepatitis, human and simian immunodeficiency viruses (HIV and SIV), fluorescent T cell tracking, and various immune responses.
Dr. Dan R. Littman, New York University Medical Center
Genetic Background | Generation |
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N13+N2F4
|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Cxcr6 | chemokine (C-X-C motif) receptor 6 |
Mice homozygous for this EGFP "knock-in" are viable, fertile, normal in size, and do not display any behavioral abnormalities when maintained under barrier conditions. Lymph nodes and spleen show no endogenous gene expression. Lymphocytes from heterozygotes, but not homozygotes, show endogenous ligand binding. EGFP is expressed in cell subsets similar to human Bonzo/STRL33, namely in T cells, particularly those displaying markers of effector/memory cells and those stimulated by IL-2.
CXCR6-deficient mice exhibit a reduction of CD1d-reactive NKT cells in the liver and decreased susceptibility to T-cell-dependent hepatitis. They are protected from liver fibrosis; macrophage infiltration and protein levels of inflammatory cytokines IFN-gamma, TNF-alpha, and IL-4 are reduced in fibrotic livers of Cxcr6-/- mice. Liver and lung NKT cells are depleted in homozygous and heterozygous Cxcr6tm1Litt mice.
Metastasis of LLC and B16 tumor cells to the liver is enhanced in Cxcr6-/- mice. This mutant may be useful in studies of hepatitis, HIV, SIV, fluorescent T cell tracking, and various immune responses.
A targeting vector containing an enhanced green fluorescent protein (EGFP, Clonetech)/SV40 poly(A)n cassette with a downstream loxP-flanked neomycin resistance gene and herpes simplex virus thymidine kinase was used to replace the endogenous coding sequence. The construct was electroporated into the 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a Cre recombinase-expressing vector before being injected into C57BL/6 blastocysts. The resulting chimeric mice were bred to C57BL/6 mice. The donating investigator stated that heterozygotes were backcrossed to C57BL/6 mice (see SNP note below) for 13 generations before being made homozygous.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | EGFP expression mimics endogenous gene expression and is detected in the spleen and lymph nodes, specifically activated/memory T cells. |
Allele Name | targeted mutation 1, Daniel Littman |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | Bonzo-; Bz-; cxcr6gfp; Cxcr6tm1Unu |
Gene Symbol and Name | Cxcr6, chemokine (C-X-C motif) receptor 6 |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | EGFP expression mimics endogenous gene expression and is detected in the spleen and lymph nodes, specifically activated/memory T cells. |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 9 |
Molecular Note | The coding sequence was replaced with an EGFP. RT-PCR failed to detect transcript in mutants. Strong GFP expression was detected in spleen and lymphoid nodes. |
Mutations Made By | Dr. Dan Littman, New York University Medical Center |
When maintaining a live colony, these mice are maintained as homozygotes.
When using the Bonzo KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #005693 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous for Cxcr6<tm1Litt> |
Frozen Mouse Embryo | B6.129P2-Cxcr6<tm1Litt>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Cxcr6<tm1Litt>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Cxcr6<tm1Litt>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.129P2-Cxcr6<tm1Litt>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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