Overview

Also Known As: Bonzo KO

In this targeted reporter strain, the Cxcr6 coding region was replaced by EGFP cDNA. EGFP is expressed on liver leukocytes, but is not seen on other leukocytes like macrophages and neutrophils. EGFP expression is seen on natural killer T (NKT) cells of the liver. This mutant may be useful in studies of hepatitis, human and simian immunodeficiency viruses (HIV and SIV), fluorescent T cell tracking, and various immune responses.

Donating Investigator

Dr. Dan R. Littman, New York University Medical Center

Genetic overview

Genetic Background	Generation
	N13+N1F14 (2019-08-26 00:00:00)

Cxcr6^tm1Litt

Allele Type	Gene Symbol	Gene Name
Targeted (Reporter, Null/Knockout)	Cxcr6	chemokine (C-X-C motif) receptor 6

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Research Applications

Research Tools
Immunology, Inflammation and Autoimmunity Research

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Base Price

Starting at:

$278.00 Domestic price for female 4-week

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Details

Detailed Description

Mice homozygous for this EGFP "knock-in" are viable, fertile, normal in size, and do not display any behavioral abnormalities when maintained under barrier conditions. Lymph nodes and spleen show no endogenous gene expression. Lymphocytes from heterozygotes, but not homozygotes, show endogenous ligand binding. EGFP is expressed in cell subsets similar to human Bonzo/STRL33, namely in T cells, particularly those displaying markers of effector/memory cells and those stimulated by IL-2.

CXCR6-deficient mice exhibit a reduction of CD1d-reactive NKT cells in the liver and decreased susceptibility to T-cell-dependent hepatitis. They are protected from liver fibrosis; macrophage infiltration and protein levels of inflammatory cytokines IFN-gamma, TNF-alpha, and IL-4 are reduced in fibrotic livers of Cxcr6^-/- mice. Liver and lung NKT cells are depleted in homozygous and heterozygous Cxcr6^tm1Litt mice.

Metastasis of LLC and B16 tumor cells to the liver is enhanced in Cxcr6^-/- mice. This mutant may be useful in studies of hepatitis, HIV, SIV, fluorescent T cell tracking, and various immune responses.

Development

A targeting vector containing an enhanced green fluorescent protein (EGFP, Clonetech)/SV40 poly(A)n cassette with a downstream loxP-flanked neomycin resistance gene and herpes simplex virus thymidine kinase was used to replace the endogenous coding sequence. The construct was electroporated into the 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a Cre recombinase-expressing vector before being injected into C57BL/6 blastocysts. The resulting chimeric mice were bred to C57BL/6 mice. The donating investigator stated that heterozygotes were backcrossed to C57BL/6 mice (see SNP note below) for 13 generations before being made homozygous.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Expression Data

Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	EGFP expression mimics endogenous gene expression and is detected in the spleen and lymph nodes, specifically activated/memory T cells.

Control Suggestions

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Unutmaz D; Xiang W; Sunshine MJ; Campbell J; Butcher E; Littman DR. 2000. The primate lentiviral receptor Bonzo/STRL33 is coordinately regulated with CCR5 and its expression pattern is conserved between human and mouse. J Immunol 165(6):3284-92PubMed: 10975845 MGI: J:104242

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Genetics

Cxcr6^tm1Litt

Allele Symbol: Cxcr6^tm1Litt

Allele Name	targeted mutation 1, Daniel Littman
Allele Type	Targeted (Reporter, Null/Knockout)
Allele Synonym(s)	targeted mutation 1, Daniel Littman; Cxcr6^tm1Litt
Gene Symbol and Name	Cxcr6, chemokine (C-X-C motif) receptor 6
Gene Synonym(s)	STRL33; BB217514; expressed sequence BB217514; TYMSTR; CD186; BONZO
Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	EGFP expression mimics endogenous gene expression and is detected in the spleen and lymph nodes, specifically activated/memory T cells.
Strain of Origin	129P2/OlaHsd
Chromosome	9
Molecular Note	The coding sequence was replaced with an EGFP. RT-PCR failed to detect transcript in mutants. Strong GFP expression was detected in spleen and lymphoid nodes.
Mutations Made By	Dr. Dan Littman, New York University Medical Center

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Research Tools
- Genetics Research
  - Tissue/Cell Markers: T cell specific surface marker
  - Tissue/Cell Markers
- Immunology, Inflammation and Autoimmunity Research
  - T cell specific surface marker
  - AIDS research tool
- Fluorescent Proteins
Immunology, Inflammation and Autoimmunity Research
- Mechanisms of HIV Infection

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Cxcr6^tm1Litt/Cxcr6^tm1Litt
involves: 129P2/OlaHsd * C57BL/6

normal phenotype

no abnormal phenotype detected
- Normal - in a specific pathogen-free environment mice are viable with normal immune cell populations, T cell proliferation, and IgM and IgG1 levels

References

Unutmaz D; Xiang W; Sunshine MJ; Campbell J; Butcher E; Littman DR. 2000. The primate lentiviral receptor Bonzo/STRL33 is coordinately regulated with CCR5 and its expression pattern is conserved between human and mouse. J Immunol 165(6):3284-92PubMed: 10975845 MGI: J:104242

Additional References

Geissmann F; Cameron TO; Sidobre S; Manlongat N; Kronenberg M; Briskin MJ; Dustin ML; Littman DR. 2005. Intravascular immune surveillance by CXCR6+ NKT cells patrolling liver sinusoids. PLoS Biol 3(4):e113PubMed: 15799695 MGI: J:117341

Additional - Cxcr6^tm1Litt related

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR:Cxcr6^tm1Litt
- Standard PCR:Cxcr6^tm1Litt
- Genotyping resources and troubleshooting
Dietary Information
- LabDiet® 5K52 formulation (6% fat)
Breeding Considerations

When maintaining a live colony, these mice are maintained as homozygotes.
- Additional Breeding and Husbandry Support
Mating System
- Homozygote x Homozygote
Citation
When using the Bonzo KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #005693 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX10 (Standard)

Pricing & Availability

Available

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous for Cxcr6<tm1Litt>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Related Products and Services

Frozen Mouse Embryo	B6.129P2-Cxcr6<tm1Litt>/J Frozen Embryos	$2595.00
Frozen Mouse Embryo	B6.129P2-Cxcr6<tm1Litt>/J Frozen Embryos	$2595.00
Frozen Mouse Embryo	B6.129P2-Cxcr6<tm1Litt>/J Frozen Embryos	$3373.50
Frozen Mouse Embryo	B6.129P2-Cxcr6<tm1Litt>/J Frozen Embryos	$3373.50

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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General Terms and Conditions

Questions about Terms of Use

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Licensing Information

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The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

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Also Known As: Bonzo KO

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Cxcr6tm1Litt

Allele Symbol: Cxcr6tm1Litt

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Cxcr6tm1Litt/Cxcr6tm1Littinvolves: 129P2/OlaHsd * C57BL/6

normal phenotype

References

Additional References

Additional - Cxcr6tm1Litt related

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Cxcr6^tm1Litt

Allele Symbol: Cxcr6^tm1Litt

Genotype: Cxcr6^tm1Litt/Cxcr6^tm1Litt
involves: 129P2/OlaHsd * C57BL/6

Additional - Cxcr6^tm1Litt related