These NG-BAC transgenic mice express enhanced green fluorescent protein (EGFP) directed by the Rag2 promoter, and may be used in studies of B and T cell development as EGFP fluorescence reflects Rag2 protein expression.
Michel C Nussenzweig, The Rockefeller University
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
Mice hemizygous for the NG-BAC transgene are viable and fertile, with expression of enhanced green fluorescent protein (EGFP) directed by the Rag2 promoter. EGFP expression is high in pro-B cells and decreases during B cell maturation. Similarly, EGFP induction in T cells occurs in the late CD4/CD8 double negative stage and expression decreases during maturation. While mature cells do not express EGFP, lingering protein can be detected. The transgene does not hinder B cell function or T cell proliferation. These NG-BAC transgenic mice can be used in studies of B and T cell development as EGFP fluorescence reflects Rag2 protein expression.
A bacterial artificial chromosome (BAC) encoding the mouse Rag1 and Rag2 sequence was modified by the insertion of an enhanced green fluorescent protein gene into exon 1 of Rag2 at the initiator methionine. The BAC was injected into FVB/N embryos and then implanted into pseudopregnant ICR females. Founder line 1 was obtained and backcrossed to FVB/N mice for more than 10 generations before being maintained by transgenic positive sibling intercross.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | EGFP expression is high in pro-B cells and decreases during B cell maturation. Similarly, EGFP induction in T cells occurs in the late CD4/CD8 double negative stage and expression decreases during maturation. |
Allele Name | transgene insertion 1, Michel C Nussenzweig |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | NG-BAC; RAG2/GFP; Rag2pGFP; RAG2p-GFP |
Gene Symbol and Name | Tg(Rag2-EGFP)1Mnz, transgene insertion 1, Michel C Nussenzweig |
Gene Synonym(s) | |
Promoter | Rag2, recombination activating 2, rat |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | EGFP expression is high in pro-B cells and decreases during B cell maturation. Similarly, EGFP induction in T cells occurs in the late CD4/CD8 double negative stage and expression decreases during maturation. |
Strain of Origin | Not Specified |
Chromosome | UN |
Molecular Note | A mouse BAC clone Containing Rag1 and Rag2 was obtained from Research Genetics. An EGFP cDNA was inserted in this clone at the start codon of Rag2 by homologous recombination. A termination codon is present at the end of the EGFP sequence. 3 lines were generated and found to have identical GFP expression. This is a representative transgene for all 3 lines. |
Mutations Made By | Michel Nussenzweig, The Rockefeller University |
When maintaining a live colony, hemizygous mice may be bred to wildtype siblings or to FVB/NJ inbred mice.
When using the FVB-Tg(Rag2-EGFP)1Mnz/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #005688 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Rag2-EGFP)1Mnz/ |
Frozen Mouse Embryo | FVB-Tg(Rag2-EGFP)1Mnz/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(Rag2-EGFP)1Mnz/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(Rag2-EGFP)1Mnz/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | FVB-Tg(Rag2-EGFP)1Mnz/J Frozen Embryo | $3373.50 |
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