FVB-Tg(Rag2-EGFP)1Mnz/J

Stock number: 005688
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These NG-BAC transgenic mice express enhanced green fluorescent protein (EGFP) directed by the Rag2 promoter, and may be used in studies of B and T cell development as EGFP fluorescence reflects Rag2 protein expression.

Detailed description

Mice hemizygous for the NG-BAC transgene are viable and fertile, with expression of enhanced green fluorescent protein (EGFP) directed by the Rag2 promoter. EGFP expression is high in pro-B cells and decreases during B cell maturation. Similarly, EGFP induction in T cells occurs in the late CD4/CD8 double negative stage and expression decreases during maturation. While mature cells do not express EGFP, lingering protein can be detected. The transgene does not hinder B cell function or T cell proliferation. These NG-BAC transgenic mice can be used in studies of B and T cell development as EGFP fluorescence reflects Rag2 protein expression.

Development

A bacterial artificial chromosome (BAC) encoding the mouse Rag1 and Rag2 sequence was modified by the insertion of an enhanced green fluorescent protein gene into exon 1 of Rag2 at the initiator methionine. The BAC was injected into FVB/N embryos and then implanted into pseudopregnant ICR females. Founder line 1 was obtained and backcrossed to FVB/N mice for more than 10 generations before being maintained by transgenic positive sibling intercross.

Allele

Symbol: Tg(Rag2-EGFP)1Mnz
Name: transgene insertion 1, Michel C Nussenzweig
MGI accession ID: MGI:3784416
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Rag2-EGFP)1Mnz
Marker name: transgene insertion 1, Michel C Nussenzweig
Chromosome: UN
Strain of origin: Not Specified
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: EGFP expression is high in pro-B cells and decreases during B cell maturation. Similarly, EGFP induction in T cells occurs in the late CD4/CD8 double negative stage and expression decreases during maturation.
Molecular note: A mouse BAC clone Containing Rag1 and Rag2 was obtained from Research Genetics. An EGFP cDNA was inserted in this clone at the start codon of Rag2 by homologous recombination. A termination codon is present at the end of the EGFP sequence. 3 lines were generated and found to have identical GFP expression. This is a representative transgene for all 3 lines.