Overview

Also Known As:NOD.Thy1^a-Clone 4 TCR

These NOD.clone-4 TCR mice express a T cell receptor specific for influenza virus A/PR/8 hemagglutinin in the context of the MHC class I molecule H2-K^d. The presence of Thy1^a serves as a marker for following donor CD8⁺ T cells in vitro.

Donating Investigator

Linda Sherman, The Scripps Research Institute

Genetic overview

Genetic Background	Generation

Thy1^a

Allele Type	Gene Symbol	Gene Name
Not Applicable	Thy1	thymus cell antigen 1, theta

Tg(TcraCl4,TcrbCl4)1Shrm

Allele Type
Transgenic (Inserted expressed sequence)

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Research Applications

Research Tools
Immunology, Inflammation and Autoimmunity Research
Hematological Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Transgenic mice, commonly referred to as NOD.clone-4 TCR, are viable, fertile, normal in size, normoglycemic and do not display any gross physical or behavioral abnormalities. The TCR expressed from this transgene is specific for influenza virus A/PR/8 hemagglutinin (HA) in the context of the MHC class I molecule H2-K^d. FACS analysis of pancreatic lymph node indicates CFSE treated NOD clone-4 T cells adoptively transferred into NOD.InsHA (Stock No. 005685) proliferate vigorously and there is a 2-3-fold increase in the percentage of dividing clone-4 TCR, CD8⁺ T cells when compared to BALB/cBy (Stock Nos. 005307 and 005533) or B10.D2 (Stock Nos. 005308 and 005534). HA specific reactivity not tolerance is observed.

This mouse is further modified with the Thy1^a allele, rather than the alternate allele Thy1^b present in C57BL/10, DBA/2, BALB/c and NOD/Lt mice. Thus, cell populations derived from these transgenic mice can be distinguished from syngeneic host and other mice with the alternate allele via flow cytometry. The presence of Thy1^a serves as a marker for following donor CD8⁺ T cells in vitro.

This transgenic model is useful in the study of T-cell recognition of defined antigen, specifically HA, in the islets of NOD-InsHA mice.

Development

The Tcra and Tcrb transgenes encode a Tcr cDNA from Clone 4 derived from a B10.D2-Hc¹ H2^d H2-T18^c/nSn mouse previously immunized with Influenza virus A/PR/8. The clone 4 transgenic constructs were co-injected and co-inserted in (C57BL/6 x BALB/c)F1 oocytes. Transgene positive mice were backcrossed to BALB/cBy-Thy1^a congenic prior to crossing to NOD/Lt for 19 generations. In 2005, The Jackson Laboratory imported this transgenic strain at generation N19F4. Upon arrival the strain was mated to NOD/LtJ (stock No. 001976) prior to mating Tg/? x Tg/?. FACS analysis performed at The Jackson Laboratory confirms Thy1^a allele is homozygous.

Expression Data

Expressed Gene	Tcrb, T cell receptor beta chain, mouse, laboratory
Expressed Gene	Tcra, T cell receptor alpha chain, mouse, laboratory
Site of Expression	T-cells.

Control Suggestions

001976 NOD/ShiLtJ

Additional Information

Considerations for Choosing Controls

Selected References

Martinez X; Kreuwel HT; Redmond WL; Trenney R; Hunter K; Rosen H; Sarvetnick N; Wicker LS; Sherman LA. 2005. CD8+ T Cell Tolerance in Nonobese Diabetic Mice Is Restored by Insulin-Dependent Diabetes Resistance Alleles. J Immunol 175(3):1677-85PubMed: 16034108 MGI: J:100008

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Genetics

Thy1^a

Allele Symbol: Thy1^a

Allele Name	a variant
Allele Type	Not Applicable
Allele Synonym(s)	thetaAKR; theta-AKR; Thy1.1; Thy-1.1; Thy1a
Gene Symbol and Name	Thy1, thymus cell antigen 1, theta
Gene Synonym(s)
Site of Expression	The Thy1 locus determines a surface antigen present on cells of the thymus, a number of mouse leukemias, brain, and in lesser amounts on lymph node and spleen cells.
Strain of Origin	Not Applicable
Chromosome	9
General Note	The Thy1 locus determines a surface antigen present on cells of the thymus, a number of mouse leukemias, brain, and in lesser amounts on lymph node and spleen cells. The allele Thy1^a determines an antigenic specificity, Thy-1.1, found in the AKR and RF strains; the allele Thy1^b determines an antigenic specificity, Thy-1.2, found in the C3HeB/Fe and many other strains (J:5243, J:5012, J:4469). The Thy1 antigen is probably present on all T lymphocytes and absent from all B lymphocytes, and it thus serves as a valuable T-cell marker (J:5243). It is very widely used in experiments designed to determine the distribution and function of T-cells. Thy1 specifies a T-cell surface glycoprotein, T25, with a molecular weight of 25 kDa (J:5707). The protein appears to be anchored in the cell membrane by a lipid that is either phosphotidylinositol or closely related to it (J:12016). Thy1 may function in the cell membrane as a signal transduction molecule (J:8333). The Thy1 locus, or possibly a gene closely linked to it, controls quantitative expression of a protein that is the same size as Thy1 and is expressed on thymus and brain but not on lymph node and spleen cells (J:7900).
Molecular Note	The allele Thy1^a determines an antigenic specificity, Thy-1.1, found in the AKR and RF strains.

Tg(TcraCl4,TcrbCl4)1Shrm

Allele Symbol: Tg(TcraCl4,TcrbCl4)1Shrm

Allele Name	transgene insertion 1, Linda Sherman
Allele Type	Transgenic (Inserted expressed sequence)
Allele Synonym(s)	(HA)-TCR; C4; CL4; CL-4; CL-4 TCR; CL4-TCR; Clone 4; clone 4 TCR; Clone-4 TCR
Gene Symbol and Name	Tg(TcraCl4,TcrbCl4)1Shrm, transgene insertion 1, Linda Sherman
Gene Synonym(s)
Promoter	Tcrb, T cell receptor beta chain, mouse, laboratory
Promoter	Tcra, T cell receptor alpha chain, mouse, laboratory
Expressed Gene	Tcrb, T cell receptor beta chain, mouse, laboratory
Expressed Gene	Tcra, T cell receptor alpha chain, mouse, laboratory
Site of Expression	T-cells.
Strain of Origin	(C57BL/6 x BALB/c)F1
Chromosome	UN
Molecular Note	Tcra and Tcrb cDNAs were isolated from Clone-4, a CTL line derived from a B10.D2-Hc¹ H2^d H2-t18^c/nSnJ mouse immunized with influenza virus A/PR/8. A Tcra cDNA fragment representing the V-J exon, containing Valpha10, was cloned into a Tcra shuttle vector that includes a mouse Tcra-V promoter, the immunoglobulin heavy-chain enhancer E_H, and the Tcra-C genes with some flanking DNA. Likewise, a Tcrb cDNA fragment representing the VDJ exon, containing Vbeta8.2, was cloned into a Tcrb shuttle vector including a mouse Tcrb-V promoter, E_H, and the Tcrb-C genes with some flanking DNA. The plasmid constructs were coinjected. This transgenic TCR specifically recognizes influenza virus A/PR/8 hemagglutinin restricted by MHC class I H2k^d.
Mutations Made By	Linda Sherman, The Scripps Research Institute

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - T Cell Receptor Transgenics
- Cancer Research
  - specific T cell deficiency
- Diabetes and Obesity Research
Immunology, Inflammation and Autoimmunity Research
- T Cell Receptor Signaling Defects
- Inflammation
- Rearranged Antigen-Specific T Cell Receptor Transgenes
- CD Antigens, Antigen Receptors, and Histocompatibility Markers
- Immunodeficiency
Hematological Research
- Immunological Defects

Genotype: Thy1^a related

Immunology, Inflammation and Autoimmunity Research
- CD Antigens, Antigen Receptors, and Histocompatibility Markers
Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - T cell specific surface marker
- Genetics Research
  - Tissue/Cell Markers: T cell specific surface marker
  - Tissue/Cell Markers

Mammalian Phenotype Terms by Genotype

References

Martinez X; Kreuwel HT; Redmond WL; Trenney R; Hunter K; Rosen H; Sarvetnick N; Wicker LS; Sherman LA. 2005. CD8+ T Cell Tolerance in Nonobese Diabetic Mice Is Restored by Insulin-Dependent Diabetes Resistance Alleles. J Immunol 175(3):1677-85PubMed: 16034108 MGI: J:100008

Additional - Thy1^a related

Additional - Tg(TcraCl4,TcrbCl4)1Shrm related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Separated PCR:Tg(TcraCl4,TcrbCl4)1Shrm
- Genotyping resources and troubleshooting
Appearance
- pink-eyed, albino
  Related Genotype: A/A Tyr^c/Tyr^c
Citation
When using the NOD.Thy1^a-Clone 4 TCR mouse strain in a publication, please cite the originating article(s) and include JAX stock #005686 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Hemizygous or Non carrierfor Tg(TcraCl4,TcrbCl4)1Shrm

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:NOD.Thy1a-Clone 4 TCR

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Thy1a

Allele Symbol: Thy1a

Tg(TcraCl4,TcrbCl4)1Shrm

Allele Symbol: Tg(TcraCl4,TcrbCl4)1Shrm

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Thy1a related

Mammalian Phenotype Terms by Genotype

References

Additional - Thy1a related

Additional - Tg(TcraCl4,TcrbCl4)1Shrm related

Genotyping Protocols

Appearance

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

Also Known As:NOD.Thy1^a-Clone 4 TCR

Thy1^a

Allele Symbol: Thy1^a

Genotype: Thy1^a related

Additional - Thy1^a related