Overview

Also Known As:NOD.Thy1^a-Clone 4 TCR

These NOD.clone-4 TCR mice express a T cell receptor specific for influenza virus A/PR/8 hemagglutinin in the context of the MHC class I molecule H2-K^d. The presence of Thy1^a serves as a marker for following donor CD8⁺ T cells in vitro.

Donating Investigator

Linda Sherman, The Scripps Research Institute

Genetic overview

Genetic Background	Generation

Tg(TcraCl4,TcrbCl4)1Shrm

Allele Type
Transgenic (Inserted expressed sequence)

Thy1^a

Allele Type	Gene Symbol	Gene Name
Not Applicable	Thy1	thymus cell antigen 1, theta

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Research Applications

Research Tools
Immunology, Inflammation and Autoimmunity Research
Hematological Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Transgenic mice, commonly referred to as NOD.clone-4 TCR, are viable, fertile, normal in size, normoglycemic and do not display any gross physical or behavioral abnormalities. The TCR expressed from this transgene is specific for influenza virus A/PR/8 hemagglutinin (HA) in the context of the MHC class I molecule H2-K^d. FACS analysis of pancreatic lymph node indicates CFSE treated NOD clone-4 T cells adoptively transferred into NOD.InsHA (Stock No. 005685) proliferate vigorously and there is a 2-3-fold increase in the percentage of dividing clone-4 TCR, CD8⁺ T cells when compared to BALB/cBy (Stock Nos. 005307 and 005533) or B10.D2 (Stock Nos. 005308 and 005534). HA specific reactivity not tolerance is observed.

This mouse is further modified with the Thy1^a allele, rather than the alternate allele Thy1^b present in C57BL/10, DBA/2, BALB/c and NOD/Lt mice. Thus, cell populations derived from these transgenic mice can be distinguished from syngeneic host and other mice with the alternate allele via flow cytometry. The presence of Thy1^a serves as a marker for following donor CD8⁺ T cells in vitro.

This transgenic model is useful in the study of T-cell recognition of defined antigen, specifically HA, in the islets of NOD-InsHA mice.

Development

The Tcra and Tcrb transgenes encode a Tcr cDNA from Clone 4 derived from a B10.D2-Hc¹ H2^d H2-T18^c/nSn mouse previously immunized with Influenza virus A/PR/8. The clone 4 transgenic constructs were co-injected and co-inserted in (C57BL/6 x BALB/c)F1 oocytes. Transgene positive mice were backcrossed to BALB/cBy-Thy1^a congenic prior to crossing to NOD/Lt for 19 generations. In 2005, The Jackson Laboratory imported this transgenic strain at generation N19F4. Upon arrival the strain was mated to NOD/LtJ (stock No. 001976) prior to mating Tg/? x Tg/?. FACS analysis performed at The Jackson Laboratory confirms Thy1^a allele is homozygous.

Expression Data

Expressed Gene	Tcrb, T cell receptor beta chain, mouse, laboratory
Expressed Gene	Tcra, T cell receptor alpha chain, mouse, laboratory
Site of Expression	T-cells.
Site of Expression	The Thy1 locus determines a surface antigen present on cells of the thymus, a number of mouse leukemias, brain, and in lesser amounts on lymph node and spleen cells.

Control Suggestions

001976 NOD/ShiLtJ

Additional Information

Considerations for Choosing Controls

Selected References

Martinez X; Kreuwel HT; Redmond WL; Trenney R; Hunter K; Rosen H; Sarvetnick N; Wicker LS; Sherman LA. 2005. CD8+ T Cell Tolerance in Nonobese Diabetic Mice Is Restored by Insulin-Dependent Diabetes Resistance Alleles. J Immunol 175(3):1677-85PubMed: 16034108 MGI: J:100008

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Genetics

Tg(TcraCl4,TcrbCl4)1Shrm

Allele Symbol: Tg(TcraCl4,TcrbCl4)1Shrm

Allele Name	transgene insertion 1, Linda Sherman
Allele Type	Transgenic (Inserted expressed sequence)
Allele Synonym(s)	(HA)-TCR; C4; CL4; CL-4; CL-4 TCR; CL4-TCR; Clone 4; clone 4 TCR; Clone-4 TCR
Gene Symbol and Name	Tg(TcraCl4,TcrbCl4)1Shrm, transgene insertion 1, Linda Sherman
Gene Synonym(s)
Promoter	Tcrb, T cell receptor beta chain, mouse, laboratory
Promoter	Tcra, T cell receptor alpha chain, mouse, laboratory
Expressed Gene	Tcrb, T cell receptor beta chain, mouse, laboratory
Expressed Gene	Tcra, T cell receptor alpha chain, mouse, laboratory
Site of Expression	T-cells.
Strain of Origin	(C57BL/6 x BALB/c)F1
Chromosome	UN
Molecular Note	Tcra and Tcrb cDNAs were isolated from Clone-4, a CTL line derived from a B10.D2-Hc¹ H2^d H2-t18^c/nSnJ mouse immunized with influenza virus A/PR/8. A Tcra cDNA fragment representing the V-J exon, containing Valpha10, was cloned into a Tcra shuttle vector that includes a mouse Tcra-V promoter, the immunoglobulin heavy-chain enhancer E_H, and the Tcra-C genes with some flanking DNA. Likewise, a Tcrb cDNA fragment representing the VDJ exon, containing Vbeta8.2, was cloned into a Tcrb shuttle vector including a mouse Tcrb-V promoter, E_H, and the Tcrb-C genes with some flanking DNA. The plasmid constructs were coinjected. This transgenic TCR specifically recognizes influenza virus A/PR/8 hemagglutinin restricted by MHC class I H2k^d.
Mutations Made By	Linda Sherman, The Scripps Research Institute

Thy1^a

Allele Symbol: Thy1^a

Allele Name	a variant
Allele Type	Not Applicable
Allele Synonym(s)	thetaAKR; theta-AKR; Thy1.1; Thy-1.1; Thy1a
Gene Symbol and Name	Thy1, thymus cell antigen 1, theta
Gene Synonym(s)
Site of Expression	The Thy1 locus determines a surface antigen present on cells of the thymus, a number of mouse leukemias, brain, and in lesser amounts on lymph node and spleen cells.
Strain of Origin	Not Applicable
Chromosome	9
General Note	The Thy1 locus determines a surface antigen present on cells of the thymus, a number of mouse leukemias, brain, and in lesser amounts on lymph node and spleen cells. The allele Thy1^a determines an antigenic specificity, Thy-1.1, found in the AKR and RF strains; the allele Thy1^b determines an antigenic specificity, Thy-1.2, found in the C3HeB/Fe and many other strains (J:5243, J:5012, J:4469). The Thy1 antigen is probably present on all T lymphocytes and absent from all B lymphocytes, and it thus serves as a valuable T-cell marker (J:5243). It is very widely used in experiments designed to determine the distribution and function of T-cells. Thy1 specifies a T-cell surface glycoprotein, T25, with a molecular weight of 25 kDa (J:5707). The protein appears to be anchored in the cell membrane by a lipid that is either phosphotidylinositol or closely related to it (J:12016). Thy1 may function in the cell membrane as a signal transduction molecule (J:8333). The Thy1 locus, or possibly a gene closely linked to it, controls quantitative expression of a protein that is the same size as Thy1 and is expressed on thymus and brain but not on lymph node and spleen cells (J:7900).
Molecular Note	The allele Thy1^a determines an antigenic specificity, Thy-1.1, found in the AKR and RF strains.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - T Cell Receptor Transgenics
- Cancer Research
  - specific T cell deficiency
- Diabetes and Obesity Research
Immunology, Inflammation and Autoimmunity Research
- T Cell Receptor Signaling Defects
- Inflammation
- Rearranged Antigen-Specific T Cell Receptor Transgenes
- CD Antigens, Antigen Receptors, and Histocompatibility Markers
- Immunodeficiency
Hematological Research
- Immunological Defects

Genotype: Thy1^a related

Immunology, Inflammation and Autoimmunity Research
- CD Antigens, Antigen Receptors, and Histocompatibility Markers
Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - T cell specific surface marker
- Genetics Research
  - Tissue/Cell Markers: T cell specific surface marker
  - Tissue/Cell Markers

Mammalian Phenotype Terms by Genotype

References

Martinez X; Kreuwel HT; Redmond WL; Trenney R; Hunter K; Rosen H; Sarvetnick N; Wicker LS; Sherman LA. 2005. CD8+ T Cell Tolerance in Nonobese Diabetic Mice Is Restored by Insulin-Dependent Diabetes Resistance Alleles. J Immunol 175(3):1677-85PubMed: 16034108 MGI: J:100008

Additional - Thy1^a related

Additional - Tg(TcraCl4,TcrbCl4)1Shrm related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Separated PCR:Tg(TcraCl4,TcrbCl4)1Shrm
- Genotyping resources and troubleshooting
Appearance
- pink-eyed, albino
  Related Genotype: A/A Tyr^c/Tyr^c
Citation
When using the NOD.Thy1^a-Clone 4 TCR mouse strain in a publication, please cite the originating article(s) and include JAX stock #005686 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Hemizygous or Non carrierfor Tg(TcraCl4,TcrbCl4)1Shrm

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Also Known As:NOD.Thy1a-Clone 4 TCR

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Tg(TcraCl4,TcrbCl4)1Shrm

Allele Symbol: Tg(TcraCl4,TcrbCl4)1Shrm

Thy1a

Allele Symbol: Thy1a

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Thy1a related

Mammalian Phenotype Terms by Genotype

References

Additional - Thy1a related

Additional - Tg(TcraCl4,TcrbCl4)1Shrm related

Genotyping Protocols

Appearance

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Also Known As:NOD.Thy1^a-Clone 4 TCR

Thy1^a

Allele Symbol: Thy1^a

Genotype: Thy1^a related

Additional - Thy1^a related