These Ap3b2 knock-out mice exhibit complex neurological and behavioral impairments and are suitable for use in applications related to the study of neuron biology.
Margit Burmeister, University of Michigan
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Ap3b2 | adaptor-related protein complex 3, beta 2 subunit |
Homozygotes are viable with diminished fertility. A truncated transcript encoding of the first 120 amino acids followed by 63 extra residues is expressed in brain tissue. Antibodies against C terminal epitopes show no endogenous protein. Homozygous mice have complex neurological and behavioral impairments including tonic-clonic seizures and increased locomotor activity after handling. Compared to wildtype, mutant mice have significantly diminished adaptor complex 3 (AP-3) in dendritic processes and 62% less presynaptic zinc storage in the CA1 stratum oriens (total 25% decrease throughout the brain). The synaptic vesicle targeting of membrane proteins involved in zinc uptake (ZnT3 and ClC-3) is compromised. This mutant may be useful in studies of neuron biology, specifically membrane protein assembly and storage in synaptic vesicles.
A targeting vector containing a neomycin resistance gene (neo) driven by the mouse phosphoglycerate kinase promoter replaced exons 5 through 12 thus removing one-third of the coding region near the N terminus of the endogenous gene. The construct was electroporated into C57BL/6-derived Bruce4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric mice were bred to C57BL/6J. Heterozygous littermates are bred to generate homozygotes, heterozygotes, and wildtype mice.
Allele Name | targeted mutation 1, Margit Burmeister |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Ap3b2- |
Gene Symbol and Name | Ap3b2, adaptor-related protein complex 3, beta 2 subunit |
Gene Synonym(s) | |
Strain of Origin | B6.Cg-Thy1a |
Chromosome | 7 |
Molecular Note | Exons 5 through 12 were targeted and one-third of the coding region near the N terminus was removed. |
Mutations Made By | Margit Burmeister, University of Michigan |
When maintaining a live colony, these mice are bred as heterozygotes, as homozygous mice have reduced fertility.
When using the Ap3b2- mouse strain in a publication, please cite the originating article(s) and include JAX stock #005684 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Ap3b2<tm1Bur> |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.