These Runx1 knock-out mice exhibit an embryonic lethal phenotype with extensive hemorrhagic lesions in the central nervous system. They are suitable for use in applications related to the study of leukemia..
Nancy Speck, University of Pennsylvania
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Runx1 | runt related transcription factor 1 |
Heterozygous mice are viable, fertile, devoid of hemorrhagic lesions, and exhibit mild hematopoietic impairment. Mice that are homozygous for the targeted mutation have an embryonic lethal phenotype; animals are alive at embryonic day 11.5, but start dying at embryonic day 12.5. Embryos exhibit extensive hemorrhagic lesions in the central nervous system including the intraventricular regions and metencephalon as well as segmental bleeds at the VII-VIII cranial nerve complex, cervical, thoracic, and caudal regions. At embryonic day 10.5, symmetrical and bilateral necrosis preceding hemorrhage is observed in homozygotes. Homozygous disruption of the endogenous gene completely blocks definitive erythropoiesis, myelopoiesis, and lymphopoiesis. This mutant may be useful in leukemia studies as disruption of the human homolog of this gene is associated with many different leukemias.
A targeting vector containing a phosphoglycerol kinase-driven neomycin resistance gene disrupted the 3.2 kb region coding for amino acids 143-178 (the final 35 amino acids in the DNA binding domain) of the endogenous gene. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and the resulting chimeric males were backcrossed to C57BL/6J females. Heterozygotes were backcrossed to (129S1/SvImJ x C57BL/6J)F1 mice for more than 10 generations and then backcrossed to C57BL/6J for at least five more generations.
Allele Name | targeted mutation 1, Nancy A Speck |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Cbfa2-; Cbfa2rd; Runx1-; Runx1deltaE4; Runx1rd |
Gene Symbol and Name | Runx1, runt related transcription factor 1 |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae |
Chromosome | 16 |
Molecular Note | Exon 4, which encodes a portion of the DNA-binding domain, was replaced with a PGK-neo cassette. RT-PCR analysis showed a lack of normal transcript in homozygous mutant mice. |
Mutations Made By | Nancy Speck, University of Pennsylvania |
When maintaining a live colony, these mice are maintained as heterozygotes. Homozygotes are embryonic lethal.
When using the Cbfa2 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #005669 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Runx1<tm1Spe> |
Frozen Mouse Embryo | B6.129S-Runx1<tm1Spe>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129S-Runx1<tm1Spe>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129S-Runx1<tm1Spe>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.129S-Runx1<tm1Spe>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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