These TEa Tg mice express a transgene containing a rearranged T cell receptor that recognizes an H2-Ea/H2-Ab complex.
Alexander Rudensky, Memorial Sloan Kettering Cancer Center
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
This transgenic strain carries a rearranged T cell receptor that recognizes a complex consisting of a short peptide derived from the H2-Ea gene (Ealpha52-68) and the H2-Ab gene product (IAb). This mouse lacks naturally occurring Ealpha52-68 peptide as the H2-Ea gene in C57BL/6J mice is incapable of expressing a protein product. Flow cytometric analysis shows 90% and 95% of CD4+ splenocytes expressing Tcra-V2 and Tcrb-V6, respectively. Mice that are hemizygous for these transgenes are viable, fertile and normal in size. This mutant mouse may be useful in studies of positive/negative selection, T cell receptor interactions, or graft-versus-host disease.
Transgenic constructs containing the mouse alpha and beta chain of the T cell receptor specific for Ealpha52-68 peptides bound to IAb were cloned from the Tcra-V2 and Tcrb-V6 expressing T cell hybridoma p8.3. The constructs were co-injected into fertilized (C57BL/6J x DBA/2)F2 eggs. Germ line transmission of the transgene was confirmed in founder line 3 and subsequently backcrossed to C57BL/6J for 23 generations.
Expressed Gene | Tcrb, T cell receptor beta chain, mouse, laboratory |
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Expressed Gene | Tcra, T cell receptor alpha chain, mouse, laboratory |
Site of Expression |
Allele Name | transgene insertion 3, Alexander Y Rudensky |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | TEa; TEa TCR; Tg(Tcra/Tcrb)1Ayr |
Gene Symbol and Name | Tg(Tcra,Tcrb)3Ayr, transgene insertion 3, Alexander Y Rudensky |
Gene Synonym(s) | |
Promoter | Tcrb, T cell receptor beta chain, mouse, laboratory |
Promoter | Tcra, T cell receptor alpha chain, mouse, laboratory |
Expressed Gene | Tcrb, T cell receptor beta chain, mouse, laboratory |
Expressed Gene | Tcra, T cell receptor alpha chain, mouse, laboratory |
Strain of Origin | (C57BL/6 x DBA/2)F2 |
Chromosome | UN |
Molecular Note | Rearranged Tcra and Tcrb cDNA sequences derived from a Valpha2Vbeta6-expressing T cell hybridoma, p8.3, were used for the transgene constructs. |
Mutations Made By | Alexander Rudensky, Memorial Sloan Kettering Cancer Center |
When maintaining a live colony, these mice are maintained as hemizygotes.
When using the B6.Cg-Tg(Tcra,Tcrb)3Ayr/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #005655 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or non-carrier for Tg(Tcra,Tcrb)3Ayr |
Frozen Mouse Embryo | B6.Cg-Tg(Tcra Tcrb)3Ayr/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Tcra Tcrb)3Ayr/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Tcra Tcrb)3Ayr/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Tcra Tcrb)3Ayr/J Frozen Embryos | $3373.50 |
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