B6.Cg-Tg(Tcra,Tcrb)3Ayr/J

Stock number: 005655
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These TEa Tg mice express a transgene containing a rearranged T cell receptor that recognizes an H2-Ea/H2-Ab complex.

Detailed description

This transgenic strain carries a rearranged T cell receptor that recognizes a complex consisting of a short peptide derived from the H2-Ea gene (Ealpha52-68) and the H2-Ab gene product (IA^b). This mouse lacks naturally occurring Ealpha52-68 peptide as the H2-Ea gene in C57BL/6J mice is incapable of expressing a protein product. Flow cytometric analysis shows 90% and 95% of CD4+ splenocytes expressing Tcra-V2 and Tcrb-V6, respectively. Mice that are hemizygous for these transgenes are viable, fertile and normal in size. This mutant mouse may be useful in studies of positive/negative selection, T cell receptor interactions, or graft-versus-host disease.

Development

Transgenic constructs containing the mouse alpha and beta chain of the T cell receptor specific for Ealpha52-68 peptides bound to IA^b were cloned from the Tcra-V2 and Tcrb-V6 expressing T cell hybridoma p8.3. The constructs were co-injected into fertilized (C57BL/6J x DBA/2)F2 eggs. Germ line transmission of the transgene was confirmed in founder line 3 and subsequently backcrossed to C57BL/6J for 23 generations.

Allele

Symbol: Tg(Tcra,Tcrb)3Ayr
Name: transgene insertion 3, Alexander Y Rudensky
MGI accession ID: MGI:2665469
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Tcra,Tcrb)3Ayr
Marker name: transgene insertion 3, Alexander Y Rudensky
Chromosome: UN
Strain of origin: (C57BL/6 x DBA/2)F2
Expressed genes: Tcra,T cell receptor alpha chain,mouse, laboratory; Tcrb,T cell receptor beta chain,mouse, laboratory
Molecular note: Rearranged Tcra and Tcrb cDNA sequences derived from a Valpha2Vbeta6-expressing T cell hybridoma, p8.3, were used for the transgene constructs.