B6C3-Del(16Cbr1-Fam3b)1Rhr/J

Stock number: 005654
Product name: Ms1Rhr
Research areas: Developmental Biology Research, Mouse/Human Gene Homologs, Neurobiology Research, Research Tools

Description

This strain is currently unavailable due to replenishing of cryopreserved stocks. Interested customers who register interest will be contacted when the strain is again available.

These mice contain a deleted segment of Chromosome 21 and are useful in studies of Down syndrome.

Detailed description

These mice are monosomic for the mouse chromosome segment orthologous to the human chromosome 21 Down syndrome critical region (DSCR). The deleted segment contains mouse orthologs of 33 conserved and minimally conserved genes in the human DSCR. The borders of the deletion are defined by the carbonyl reductase 1 (Cbr1) gene and a site adjacent to the myxovirus (influenza virus) resistance 2 (Mx2) locus. Monosomic mice are viable, fertile and are significantly smaller than wildtype (euploid) littermates from birth to adulthood. This mouse may be useful in studies of Down syndrome and further exploring the ploidy of the DSCR segment of mouse Chr 16.

Development

A targeting vector containing a 3' portion of a neomycin resistance gene, a loxP site, and a hygromycin resistance gene at the (Cbr1) locus at the proximal end of the DSCR on MMU16 was electroporated into 129S6/SvEv-derived MC1 embryonic stem (ES) cells. Correctly targeted ES cells were then electroporated with a second targeting vector designed to orient a puromycin resistance gene, a loxP site, and a 5' portion of a neomycin resistance gene adjacent to the (Mx2) locus at the distal end of MMU16. Next, a CAGGS-cre expression vector was electroporated into ES cells harboring both targeted mutations. In ES cells where each construct inserted in trans on homologous chromosomes, recombination between the loxP sites resulted in mice with a duplication of the MMU16 region between the loxP sites on one homologous chromosome and interstitial deletion of the MMU16 region between the loxP sites on the other homologous chromosome. Heterozygous ES cells were injected into blastocysts and then transferred to surrogate mothers. F1 progeny of chimeric mice were bred to (C57BL/6J x C3H/HeJ)Fn to produce mice with segmental monosomy of MMU16 (Stock# 5654 -Del(16Cbr1-ORF9)1Rhr) or segmental trisomy (see Stock# 5383 -Dp(16Cbr1-ORF9)1Rhr). Monosomic mice were crossed with (C57BL/6J x C3H/HeJ)F1 for 4 generationsbefore arriving at The Jackson Laboratory. Upon arrival, this mutant strain has been maintained by breeding with B6C3F1/J.

Allele

Symbol: Del(16Cbr1-Fam3b)1Rhr
Name: deletion, chr 16, R H Reeves 1
MGI accession ID: MGI:3487281
Generation method: Targeted
Attributes: Null/Knockout
Synonyms: Del1Rhr; Ms1Rhr
Marker symbol: Del(16Cbr1-Fam3b)1Rhr
Marker name: deletion, Chr16, R H Reeves 1
Marker synonyms: Del(16Cbr1-ORF9)1Rhr; Del(16Mx2-Cbr1)1Rhr; Del1Rhr
Chromosome: 16
Strain of origin: 129S6/SvEvTac
Molecular note: Deletion of a 3.9 Mb segment of Chr 16 was engineered by cre-mediated recombination between single loxP sites inserted into homologous chromosomes. This deleted segment contains mouse orthologs of 33 conserved and minimally conserved genes in the human Down syndrome critical region.
General note: A line of mice with segmental monosomy for Chr 16 was derived from descendants of the chimeric founder.